Pathogenic Bacteria Are the Primary Determinants Shaping PM2.5-Borne Resistomes in the Municipal Food Waste Treatment System DOI
Liangmao Zhang, Binghan Wang, Yinglong Su

et al.

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(48), P. 19965 - 19978

Published: Nov. 16, 2023

Bioaerosol pollution poses a substantial threat to human health during municipal food waste (FW) recycling. However, bioaerosol-borne antibiotic-resistant genes (ARGs) have received little attention. Herein, 48 metagenomic data were applied study the prevalence of PM2.5-borne ARGs in and around full-scale treatment plants (FWTPs). Overall, FWTP PM2.5 (2.82 ± 1.47 copies/16S rRNA gene) harbored comparable total abundance that wastewater plant (WWTP), but was significantly enriched with multidrug type (e.g., AdeC/I/J; p < 0.05), especially abundant could serve as effective indicators define resistome profiles FWTPs (Random Forest accuracy >92%). exhibited decreasing enrichment along FWTP–downwind–boundary gradient, eventually reaching levels urban (1.46 0.21 gene, N = 12). The combined analysis source-tracking, metagenome-assembled genomes (MAGs), culture-based testing provides strong evidence Acinetobacter johnsonii-dominated pathogens contributed shaping disseminating ARGs, while abiotic factors (i.e., SO42–) indirectly participated these processes, which deserves more attention developing strategies mitigate airborne ARGs. In addition, exposure level resistant about 5–11 times higher than those PM2.5, be severe hospital certain scenarios (<41.53%). This work highlights importance need for re-evaluating air induced by public terms.

Language: Английский

PCycDB: a comprehensive and accurate database for fast analysis of phosphorus cycling genes DOI Creative Commons
Jiaxiong Zeng, Qichao Tu, Xiaoli Yu

et al.

Microbiome, Journal Year: 2022, Volume and Issue: 10(1)

Published: July 4, 2022

Abstract Background Phosphorus (P) is one of the most essential macronutrients on planet, and microorganisms (including bacteria archaea) play a key role in P cycling all living things ecosystems. However, our comprehensive understanding genes (PCGs) (PCMs) as well their ecological functions remains elusive even with rapid advancement metagenome sequencing technologies. One major challenges lack accurately annotated functional gene database. Results In this study, we constructed well-curated database (PCycDB) covering 139 families 10 metabolic processes, including several previously ignored PCGs such pafA encoding phosphate-insensitive phosphatase, ptxABCD (phosphite-related genes), novel aepXVWPS for 2-aminoethylphosphonate transporters. We achieved an annotation accuracy, positive predictive value (PPV), sensitivity, specificity, negative (NPV) 99.8%, 96.1%, 99.9%, respectively, simulated datasets. Compared to other orthology databases, PCycDB more accurate, comprehensive, faster profile PCGs. used analyze microbial communities from representative natural engineered environments showed that could apply different environments. Conclusions demonstrate powerful tool advancing microbially driven environment high coverage, analysis data. The available at https://github.com/ZengJiaxiong/Phosphorus-cycling-database .

Language: Английский

Citations

89
Active predation, phylogenetic diversity, and global prevalence of myxobacteria in wastewater treatment plants DOI Creative Commons
Lu Zhang, Xinyu Huang, Jizhong Zhou

et al.

The ISME Journal, Journal Year: 2023, Volume and Issue: 17(5), P. 671 - 681

Published: Feb. 11, 2023

Abstract The operation of modern wastewater treatment plants (WWTPs) is driven by activated sludge microbiota, a complex assemblage trophically interacting microorganisms. Microbial predation crucial to fundamental understanding how biological interactions drive microbiome structuring and functioning WWTPs. However, predatory bacteria have received little attention regarding their diversity, activity, ecological function in sludge, limiting the exploitation food web for engineering. Here, using rRNA-stable isotope probing microbiota with 13C-labeled prey bacteria, we uncovered diverse as-yet-uncultivated putative that actively incorporated 13C-biomass. Myxobacteria, especially Haliangium mle1-27 clade, were found as dominant active predators, refreshing conventional views based on few isolates Bdellovibrionota from identified showed more selective compared protists dominated ciliates, providing situ evidence inter-domain behavior divergence sludge. Putative tracked over two-year monitoring effort at local WWTP, revealing predominance Myxococcota (6.5 ± 1.3%) (1.0 0.2%) lineages. Phylogenetic analysis unveiled highly myxobacteria inhabiting suggested habitat filtering effect global Further mining dataset revealed prevalence (5.4 0.1%) species potential impacts myxobacterial process performance. Collectively, our findings provided unique insights into predating highlighting links processes via trophic regulation enteric functional bacteria.

Language: Английский

Citations

60
Deciphering basic and key traits of antibiotic resistome in influent and effluent of hospital wastewater treatment systems DOI
Lin Zhu, Ling Yuan,

Xinyi Shuai

et al.

Water Research, Journal Year: 2023, Volume and Issue: 231, P. 119614 - 119614

Published: Jan. 14, 2023

Language: Английский

Citations

54
Nitric Oxide: A Neglected Driver for the Conjugative Transfer of Antibiotic Resistance Genes among Wastewater Microbiota DOI
Haining Huang,

Guanqun Feng,

Meng Wang

et al.

Environmental Science & Technology, Journal Year: 2022, Volume and Issue: 56(10), P. 6466 - 6478

Published: May 5, 2022

The dissemination of plasmid-borne antibiotic resistance genes (ARGs) in wastewater is becoming an urgent concern. Previous studies mainly focused on the effects coexisting contaminants plasmid conjugation, but ignored potential contribution some byproducts inevitably released from treatment processes. Herein, we demonstrate for first time that nitric oxide (NO), intermediate nitrogen cycle, can significantly boost conjugative transfer RP4 Escherichia coli K12 to different recipients (E. HB101, Salmonella typhimurium, and microbiota). Phenotypic genotypic tests confirmed NO-induced promotion was not attributed SOS response, a well-recognized driver horizontal gene transfer. Instead, NO exposure increased outer membrane permeability both donor recipient by inhibiting expression key involved lipopolysaccharide biosynthesis (such as waaJ), thereby lowering barrier conjugation. On other hand, only resulted accumulation intracellular tryptophan also triggered deficiency methionine, which were validated play roles regulating global regulatory (korA, korB, trbA) RP4, activating its encoding apparatus (represented trfAp trbBp). Overall, our findings highlighted risks spreading ARGs among microbiota updated regulation mechanism

Language: Английский

Citations

39
Potential Auxiliary Metabolic Capabilities and Activities Reveal Biochemical Impacts of Viruses in Municipal Wastewater Treatment Plants DOI
Ling Yuan, Feng Ju

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(13), P. 5485 - 5498

Published: March 22, 2023

Viruses influence biogeochemical cycles in oceans, freshwater, soil, and human gut through infection by modulating virocell metabolism virus-encoded auxiliary metabolic genes (vAMGs). However, the geographical distribution, potential function, engineering significance of vAMGs wastewater treatment plants (WWTPs) remain to be explored. Here, 752 single-contig viral genomes with high confidence, 510 which belonged Caudovirales, were recovered from activated sludge metagenomes 32 geographically distributed WWTPs. A total 101 involved various pathways identified, most common queuosine biosynthesis folE, queD, queE sulfur gene cysH. Phylogenetic analysis virus–host relationship prediction revealed probable evolutionary histories carbon (acpP prsA), nitrogen (amoC), (cysH), phosphate (phoH) metabolism, potentially mediate microbial nutrient cycling. Notably, 11 38 (28.3%) identified corresponding metatranscriptomes transcriptionally expressed, implying an active functional state. This meta-analysis provides first broad catalog municipal WWTPs how they may assist basic physiological reactions their hosts or cycling WWTPs, therefore, have important effects on processes.

Language: Английский

Citations

32
Metagenomic surveillance of antibiotic resistome in influent and effluent of wastewater treatment plants located on the Qinghai-Tibetan Plateau DOI
Bin Shi, Renxin Zhao,

Guijin Su

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 870, P. 162031 - 162031

Published: Feb. 4, 2023

Language: Английский

Citations

28
Size-dependent promotion of micro(nano)plastics on the horizontal gene transfer of antibiotic resistance genes in constructed wetlands DOI

Yanhui Zhao,

Zhen Hu, Huimin Xie

et al.

Water Research, Journal Year: 2023, Volume and Issue: 244, P. 120520 - 120520

Published: Aug. 24, 2023

Language: Английский

Citations

27
Differential effects of wastewater treatment plant effluents on the antibiotic resistomes of diverse river habitats DOI Creative Commons
Jangwoo Lee, Feng Ju,

Karin Beck

et al.

The ISME Journal, Journal Year: 2023, Volume and Issue: 17(11), P. 1993 - 2002

Published: Sept. 8, 2023

Abstract Wastewater treatment plants (WWTPs) are key sources of antimicrobial resistance genes (ARGs) that could influence the resistomes microbial communities in various habitats receiving river ecosystem. However, it is currently unknown which most impacted and whether ARGs, like certain chemical contaminants, be accumulated or enriched We conducted a systematic metagenomic survey on antibiotic WWTP effluent, four riverine (water, suspended particles, sediment, epilithic biofilm), freshwater amphipod gut microbiomes. The impact effluent downstream was assessed nine Swiss rivers. While there were significant differences across habitats, wastewater resistome more similar to water than other habitat strongly by WWTPs effluent. sulfonamide, beta-lactam, aminoglycoside among abundant ARGs effluents, especially aadA, sul1, class A beta-lactamase showed significantly increased abundance compared upstream locations (p &lt; 0.05). this not case for biofilm, habitats. Accordingly, evidence accumulation enrichment through food web identified. Our study suggests monitoring determinants using “co-occurrence” as an indicator wastewater-related pollution should focus affected habitat.

Language: Английский

Citations

27
Bacteriophage-based biosensors for detection of pathogenic microbes in wastewater DOI

Karthik Basthi Shivaram,

Pankaj Bhatt,

Mohit S. Verma

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 901, P. 165859 - 165859

Published: July 27, 2023

Language: Английский

Citations

25
Exploring diversity patterns and driving mechanisms of the antibiotic resistome and microbiome in saline groundwater DOI

Moran Tang,

Qian Chen, Haohui Zhong

et al.

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 446, P. 130734 - 130734

Published: Jan. 6, 2023

Language: Английский

Citations

23