Ecotoxicology and Environmental Safety,
Journal Year:
2023,
Volume and Issue:
263, P. 115348 - 115348
Published: Aug. 17, 2023
Organophosphate
flame
retardants
(OPFRs)
are
environmental
pollutants
of
increasing
interest,
widely
distributed
in
the
environment
and
exerting
possible
deleterious
effects
towards
human
health.
The
present
study
investigates
vitro
their
interactions
with
drug
transporters,
which
targets
for
chemicals
actors
toxicokinetics.
Some
OPFRs,
i.e.,
tris(2-butoxyethyl)
phosphate
(TBOEP),
tris(1,3-dichloroisopropyl)
(TDCPP),
tri-o-cresyl
(TOCP)
triphenyl
(TPHP),
were
found
to
inhibit
activities
some
such
as
organic
anion
transporter
3
(OAT3),
transporting
polypeptide
(OATP)
1B1,
OATP1B3,
cation
2
(OCT2)
or
breast
cancer
resistance
protein
(BCRP).
These
concentration-dependent,
IC50
values
ranging
from
6.1
µM
(for
TDCPP-mediated
inhibition
OCT2)
51.4
TOCP-mediated
BCRP).
OPFRs
also
blocked
transporter-dependent
membrane
passage
endogenous
substrates,
notably
that
hormones.
OAT3
however
failed
transport
TBOEP
TPHP.
additionally
repressed
mRNA
expressions
transporters
cultured
hepatic
HepaRG
cells,
especially
those
OAT2
OCT1
response
TOCP,
2.3
2.5
µM,
respectively.
data
therefore
add
expanding
list
interacting
even
if
OPFR
concentrations
required
impact
2–50
range,
rather
higher
than
observed
humans
environmentally
dietarily
exposed
these
chemicals.
Environmental Science & Technology,
Journal Year:
2024,
Volume and Issue:
58(31), P. 13648 - 13657
Published: July 29, 2024
Organophosphorus
flame
retardants
(OPFRs)
such
as
triphenyl
phosphate
(TPHP)
and
tris(1,3-dichloro-2-propyl)
(TDCIPP)
were
reported
to
impair
cardiac
function
in
fish.
However,
limited
information
is
available
regarding
their
cardiotoxic
mechanisms.
Using
rare
minnow
(Gobiocypris
rarus)
a
model,
we
found
that
both
TPHP
TDCIPP
exposures
decreased
heart
rate
at
96
h
postfertilization
(hpf)
embryos.
Atropine
(an
mAChR
antagonist)
can
significantly
attenuate
the
bradycardia
caused
by
TPHP,
but
only
marginally
attenuated
treatment,
suggesting
TDCIPP-induced
independent
of
mAChR.
Unlike
TDCIPP,
although
TPHP-induced
could
be
reversed
transferring
larvae
clean
medium,
inhibitory
effect
AChE
activity
persisted
compared
hpf,
indicating
existence
other
regulatory
Transcriptome
profiling
revealed
cardiotoxicity-related
pathways
treatments
24
72
hpf
embryos/larvae.
Similar
transcriptional
alterations
also
confirmed
hearts
adult
Further
studies
verified
interfere
with
Na+/Ca2+
transport
lead
disorders
excitation–contraction
coupling
larvae.
Our
findings
provide
useful
clues
for
unveiling
differential
mechanisms
OPFRs
identifying
abnormal
one
select
few
known
factors
sufficient
fish
function.
Ecotoxicology and Environmental Safety,
Journal Year:
2025,
Volume and Issue:
291, P. 117877 - 117877
Published: Feb. 1, 2025
Diabetic
kidney
disease
is
a
significant
complication
of
diabetes
mellitus,
and
exposure
to
certain
chemicals
may
play
role
in
its
development.
Triphenyl
phosphate
(TPHP)
commonly
used
plastics
flame
retardants.
This
study
aims
investigate
the
potential
impact
TPHP
metabolite
diphenyl
(DPHP)
on
diabetic
using
various
methods,
including
network
toxicology,
molecular
docking,
cell
experiments
like
CCK8
assay
real-time-PCR.
The
research
examined
relationship
between
urinary
DPHP
levels
function
American
adults
data
from
National
Health
Nutrition
Examination
Survey
(NHANES)
2017
March
2020.
Additionally,
explored
targets
action
for
toxicity
analysis,
conducted
protein
interaction
functional
aspects
through
Gene
Ontology
Kyoto
Encyclopedia
Genes
Genomes
enrichment
analysis.
Furthermore,
identified
key
proteins
involved
experimental
verification
by
treating
cells
with
DPHP.
Toxicity
analysis
showed
that
could
cause
dose-dependent
mouse
podocyte
clone
5
(MPC5)
mesangial
(MES13).
also
detected
mRNA
expression
core
molecularly
docked
results
indicated
statistically
regulation
most
MPC5,
MES13,
human
kidney-2
cells.
Ecotoxicology and Environmental Safety,
Journal Year:
2025,
Volume and Issue:
291, P. 117913 - 117913
Published: Feb. 1, 2025
Intestinal
fibrosis
is
often
observed
in
inflammatory
bowel
disease
(IBD)
and
seriously
affects
intestinal
health.
Our
previous
study
identified
that
triphenyl
phosphate
(TPhP),
one
kind
of
frequently
used
organophosphate
flame
retardants
(OPFRs),
induced
IBD-like
features
colon.
Herein,
we
firstly
extracellular
matrix
deposition
colon
tissues,
indicative
appearance
colonic
fibrosis.
Further
studies
showed
TPhP
downregulated
epithelial
marker
E-cadherin
levels
but
upregulated
alpha
smooth
muscle
actin
(α-SMA)
mouse
similar
results
were
cultured
cells,
indicating
fibrogenesis
might
be
attributed
to
epithelial-mesenchymal
transition
(EMT).
transcriptome
experimental
data
demonstrated
TPhP-induced
EMT
was
closely
associated
with
activated
Wnt/β-catenin
pathway.
Moreover,
FOXM1
facilitated
the
entrance
β-catenin
into
nucleus
regulate
expression
Wnt
target
genes,
promoting
initiation.
Collectively,
these
findings
mice
by
activating
EMT,
this
work
may
provide
new
perspectives
exploring
etiology
developing
relevant
treatment
strategies.
Environmental Science & Technology,
Journal Year:
2024,
Volume and Issue:
58(35), P. 15486 - 15496
Published: Aug. 21, 2024
Aryl
phosphorus
flame
retardants
(aryl-PFRs),
such
as
triphenyl
phosphate
(TPHP)
and
diphenyl
(DPHP),
are
widely
used
worldwide.
Understanding
the
fates
of
aryl-PFRs
