Environmental Pollution, Journal Year: 2024, Volume and Issue: 363, P. 125096 - 125096
Published: Oct. 9, 2024
Language: Английский
Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 481, P. 148634 - 148634
Published: Jan. 8, 2024
Language: Английский
Citations
12
Environmental Science & Technology, Journal Year: 2024, Volume and Issue: unknown
Published: Feb. 19, 2024
Anthropogenic organohalide pollutants pose a severe threat to public health and ecosystems. In situ bioremediation using respiring bacteria (OHRB) offers an environmentally friendly cost-efficient strategy for decontaminating organohalide-polluted sites. The genomic structures of many OHRB suggest that dehalogenation traits can be horizontally transferred among microbial populations, but their occurrence anaerobic has not yet been demonstrated experimentally. This study isolates characterizes novel tetrachloroethene (PCE)-dechlorinating Sulfurospirillum sp. strain SP, distinguishing itself by showcasing mechanism essential horizontal dissemination reductive capabilities within populations. Its genetic characterization identifies unique plasmid (pSULSP), harboring dehalogenase de novo corrinoid biosynthesis operons, functions critical respiration, flanked mobile elements. active mobility these elements was through analyses spontaneously emerging nondehalogenating variants SP. More importantly, bioaugmentation microcosms with pSULSP DNA triggered PCE dechlorination in taxonomically diverse bacterial Our results directly support the hypothesis exposure anthropogenic drive emergence dehalogenating populations via gene transfer demonstrate which remediation could achieved environments.
Language: Английский
Citations
8
Microbiome, Journal Year: 2024, Volume and Issue: 12(1)
Published: March 16, 2024
Abstract Background Massive amounts of sewage sludge are generated during biological treatment and commonly subjected to anaerobic digestion, land application, landfill disposal. Concurrently, persistent organic pollutants (POPs) frequently found in disposal systems, posing significant risks both human health wildlife. Metabolically versatile microorganisms originating from inevitably introduced potentially affecting the fate POPs. However, there is currently a dearth comprehensive assessments regarding capability microbiota geographically disparate regions attenuate POPs underpinning microbiomes. Results Here we report global prevalence organohalide-respiring bacteria (OHRB) known for their capacity sludge, with an occurrence frequency ~50% investigated samples (605 1186). Subsequent laboratory tests revealed microbial reductive dechlorination polychlorinated biphenyls (PCBs), one most notorious categories POPs, 80 out 84 microcosms via various pathways. Most chlorines were removed para - meta -positions PCBs; nevertheless, ortho -dechlorination PCBs also occurred widely, although lower extents. Abundances several well-characterized OHRB genera ( Dehalococcoides , Dehalogenimonas Dehalobacter ) uncultivated Dehalococcoidia lineages increased incubation positively correlated PCB dechlorination, suggesting involvement dechlorinating PCBs. The previously identified dehalogenase (RDase) genes pcbA4 pcbA5 tended coexist microcosms, but low ratios these RDase abundance indicated existence undescribed RDases sludge. Microbial community analyses positive correlation between biodiversity activity was apparent threshold co-occurrence network complexity beyond which decreased. Conclusions Our findings that exhibited nearly ubiquitous indicate widespread nonnegligible impacts on systems. diverse suggests as alternative source obtain POP-attenuating consortia calls further exploration populations
Language: Английский
Citations
8
BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)
Published: March 31, 2025
Abstract Background Industrial waste, agricultural runoff and untreated sewage contaminate the Nile, leaving a toxic legacy in its sediments. Organohalides-polluted sediment particular poses serious public health risks detrimental effects on aquatic life. Sediment microbiomes may harbor bacterial strains that could be utilized bioremediation of such pollutants. Material methods Two from polluted River Nile sediments were analyzed by using 16S rRNA gene amplicon sequencing. In addition, PICRUSt analysis based data was used to explore organohalide respiring bacteria (OHRB) genera their corresponding respiration (OHR) activity. Microcosm studies performed validate potential for dechlorination activity sediment. Dechlorination parent chloroethenes into daughter end product detected gas chromatography coupled with flame ionization detection analysis. Results Analysis sequences EZ-biocloud server identified Proteobacteria as dominant phylum both microbiomes, Bacteroidetes Chloroflexi prevalent RNS1 Chlorobi RNS2 PCR analyses several OHRB genera, including Dehalococcoides , Dehalogenimonas Desulfomonile Desulfovibrio Geobacter suggesting OHR Further evidence provided functional prediction analysis, which suggested presence reductive dehalogenases biomarkers associated samples. Specifically, predicted genes tetrachloroethene dehalogenase 3-chloro-4-hydroxyphenylacetate dehalogenase, previously linked OHR. confirmed dichloroethene. Conclusion This study demonstrates industrialized area harbors distinct enclosing various providing substantial It also provides
Language: Английский
Citations
0
Bioremediation Journal, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 17
Published: June 4, 2024
Concentrations greater than 20 mg/L of chlorinated volatile organic compounds (cVOCs) including tetrachloroethene (PCE), trichloroethene (TCE), and cis-1,2-dichloroethene (cDCE) have been present in site groundwater for more four decades. To promote a faster clean-up time, an situ bioremediation approach was evaluated using In-Situ Microcosms® (ISMs) followed by full-scale approach. The ISM study slow-release versus quick-release carbon substrates with without bioaugmentation the ethene degrading culture, SDC-9™. After three-month incubation period, ISMs were retrieved. amended source or displayed 93% reduction TCE, which corresponded to increase cDCE all ISMs. Dehalococcoides population gene abundances associated biodegradation (tceA, bvcA, vcrA) increased three orders magnitude bioaugmented over natural attenuation only Additionally, SDC-9™ AquaBupH®, enhanced emulsified oil substrate (EOS®) buffer, unit showed highest level vinyl chloride similar EHC®, controlled-release, organo-iron substrate, ISM. However, AquaBupH acetate, demonstrating active fermentation processes. results indicated that combined biostimulation along effectively promoted conditions conducive reductive dechlorination. system coupled successfully reduce ethenes groundwater. EOS-100® served as sustained CoBupH (buffering agent AquaBupH), facilitating production hydrogen, through fatty acid fermentation. This led subsequent years, showcasing minimal rebound contaminant levels. Two rounds notably population, accelerating processes, is setting up monitored attenuation. shows guide design resulted effective cVOC quicker sustainable clean-up.
Language: Английский
Citations
1
Environmental Science & Technology, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 3, 2024
Chloroform and trichloroethanes are pervasive groundwater contaminants for which bioremediation has been an effective treatment strategy. Reductive dehalogenase (RDase) enzymes from organohalide-respiring bacteria essential their remediation under anaerobic conditions. RDases responsible dehalogenating these chlorinated solvents, leading to removal. This work explores the kinetic characteristics of three closely related Dehalobacter chloroalkane-reductases─TmrA, CfrA, AcdA─and identifies differences between activity on chloroform (CF), 1,1,1-trichloroethane (TCA), 1,1,2-TCA. The side-by-side comparison emphasized that TmrA AcdA specialized toward CF with both having a 4-fold higher maximum specific (Vmax) than 1,1,1-TCA, whereas CfrA very similar rates 1,1,1-TCA. is most sensitive substrate inhibition by 1,1,2-TCA common cocontaminant trichloroethene. Finally, reduction 1,1,2-TCA, can produce 1,2-dichloroethane vinyl chloride, was assessed each enzyme. Interestingly, enzyme distinct preference major product it produces, indicating favored reaction pathway. Despite over 95% sequence identity, TmrA, exhibit substantial in behavior, highlighting importance understanding such nuances informed strategies.
Language: Английский
Citations
1
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: July 11, 2024
ABSTRACT Chloroform and trichloroethanes are pervasive groundwater contaminants for which bioremediation has been an effective treatment strategy. Reductive dehalogenase (RDase) enzymes from organohalide-respiring bacteria essential their remediation under anaerobic conditions. RDases responsible dehalogenating these chlorinated solvents, leading to removal. This work explores the kinetic characteristics of three closely related Dehalobacter chloroalkane-reductases—TmrA, CfrA, AcdA—and identifies differences between activity on chloroform (CF), 1,1,1-trichloroethane (TCA), 1,1,2-TCA. The side-by-side comparison emphasized that TmrA AcdA specialized toward CF with both having 4-fold higher maximum specific ( V max ) than 1,1,1-TCA, whereas, CfrA very similar rates 1,1,1-TCA. is most sensitive substrate inhibition by 1,1,2-TCA, a common co-contaminant trichloroethene. Finally, reduction can produce 1,2-dichloroethane vinyl chloride, was assessed each enzyme. Interestingly, enzyme distinct preference major product it produces, indicating favoured reaction pathway. Despite over 95% sequence identity, TmrA, exhibit substantial in behaviour, highlighting importance understanding such nuances informed strategies. SYNOPSIS Three dechlorinating were found have levels sensitivity inhibition. These implications enzymes’ use bioremediation.
Language: Английский
Citations
1
Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 496, P. 154038 - 154038
Published: July 15, 2024
Language: Английский
Citations
0
Environmental Pollution, Journal Year: 2024, Volume and Issue: 363, P. 125096 - 125096
Published: Oct. 9, 2024
Language: Английский
Citations
0