The Hunt for Chemical Dark Matter across a River-to-Ocean Continuum

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(27), P. 11988 - 11997

Published: June 14, 2024

Thousands of mass peaks emerge during molecular characterization natural dissolved organic matter (DOM) using ultrahigh-resolution spectrometry. While assigned to certain formulas have been extensively studied, the uncharacterized that represent a significant fraction and convey biogenic elements energy previously ignored. In this study, we introduce term dark DOM (DDOM) for unassigned explored its characteristics environmental behaviors data set 38 extracts covering Yangtze River-to-ocean continuum. We identified total 9141 DDOM molecules, which exhibited higher weight greater diversity than subset with formulas. Although contributed smaller relative abundance, it significantly impacted composition bulk DOM. A portion was found increase abundance across river-to-ocean These compounds could contain halogenated molecules might high potential contribute refractory carbon pool. With underline contribution pool emphasize more research is needed understand global biogeochemical cycles sequestration.

Language: Английский

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Molecular dynamics and factors governing recalcitrance of dissolved organic matter: Insights from laboratory incubation and ultra-high resolution mass spectrometry

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 964, P. 178580 - 178580

Published: Jan. 24, 2025

Language: Английский

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Describing the complex chemistry of benthic seawater: from exometabolite sampling strategies to MS-based metabolomics

Natural Product Reports, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This review highlights advances in characterizing exometabolites (EMs) from benthic organisms, starting with situ sampling methods, then discussing how marine MS-based (exo)metabolomics benefits various fields while addressing ongoing challenges.

Language: Английский

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Molecular Composition of Hydroxyl Radical-Resistant Organics in Municipal Solid Waste Leachate

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: unknown, P. 137014 - 137014

Published: Dec. 1, 2024

Language: Английский

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Metabolomic signatures of pathogen suppression effect of Baltic eelgrass meadows in surrounding seawater

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 979, P. 179518 - 179518

Published: April 27, 2025

Organic molecules exuded into water column by marine organisms represent a significant portion of dissolved organic matter (DOM) that modulates biochemical interactions. Secreted allelochemicals have been suggested to be involved in regulation pathogen abundance seagrass meadows, however, exometabolome has remained unstudied. We aimed identify exometabolites, within and outside explore their potential involvement suppression under varying environmental conditions. collected seawater (SW) samples from eelgrass (Zostera marina)-vegetated (V) non-vegetated (NV) areas across 5 locations spanning 270 km coastline along the German Baltic Sea. Comparative LC-MS/MS-based untargeted computational metabolomics combined with statistical analyses machine learning tools were employed pinpoint (exo)metabolomic signatures leaves. Simultaneously, we measured abiotic parameters three common pathogenic taxa seawater, investigated spatiotemporal variations. Here show correlation biomass reduction effect increasing temperature, eutrophication anthropogenic influences. Exometabolomics studies revealed exudates contributed significantly overall DOM at molecular level, while SW overlying meadows contained many chemical features unique leaf metabolome. identified four flavone aglycones as key biomarkers distinguishing SW-V SW-NV samples. Their drastically increased concentrations correlated lowest biomass, suggesting role regulation. These analytical microbiological approaches indicate flavones are defensive released upon stress serve bioindicators eelgrass' sanitation effect.

Language: Английский

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Analysis of dissolved organic matter characteristics in pharmaceutical wastewater via spectroscopy combined with Fourier-transform ion cyclotron resonance mass spectrometry

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 479, P. 135706 - 135706

Published: Sept. 3, 2024

Language: Английский

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Online LC‐Orbitrap MS method for the rapid molecular characterization of dissolved organic matter

Rapid Communications in Mass Spectrometry, Journal Year: 2024, Volume and Issue: 38(20)

Published: Aug. 24, 2024

Rationale High‐resolution mass spectrometry (HRMS) combined with electrospray ionization (ESI) has been the most useful technique for molecular characterization of dissolved organic matter (DOM) derived from diverse sources. However, comprehensive detection DOM composition was hindered by suppression observed in ESI HRMS coupled liquid chromatography (LC) is a potential tool DOM. Methods The Suwannee River fulvic acids (SRFAs) and two samples seawater refinery wastewater extracted solid phase extraction (SPE) were analyzed LC‐Orbitrap MS ESI. mobile phases, solvent composition, gradient analysis optimized. Results number detected formulae SRFAs online significantly increased approximately 40% compared to direct injection. These additional compounds are mainly protein lignin‐like compounds, low O/C ratio high H/C ratio. For SRFAs, relative standard deviations (%) reproducibility 5.51, 2.33, 7.97, 1.80 average O/C, H/C, double bond equivalent, modified aromaticity index, respectively. Conclusions This study proposed simple rapid method based on an in‐depth DOM, achieving remarkable time only 5 min per sample. provides dependable efficient approach thereby advancing our comprehension investigation across ecosystems.

Language: Английский

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Effects of chloride ions on the transformation of dissolved organic matter in biologically treated waste leachate by a Fenton process

Separation and Purification Technology, Journal Year: 2024, Volume and Issue: unknown, P. 131321 - 131321

Published: Dec. 1, 2024

Language: Английский

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Deciphering the Energy Use Channels in Soil Organic Matter: Impacts of Long-term Farmyard Manure Addition and Microbial Necromass Revealed by LC-FT-ICR-MS

Published: June 24, 2024

Soil organic matter (SOM) plays a central role in the global carbon cycle and contributes to storage of C energy soils. Farmyard manure (FYM) addition arable soils is measure increase SOM content, microbial activity abundance metabolites (e.g., necromass (NM) markers). However, understanding mechanistic links between soil dynamics hampered due chemical complexity SOM. Non-targeted molecular-level methods like liquid chromatography coupled Fourier transform ion cyclotron resonance mass spectrometry (LC-FT-ICR-MS) can be used explore complex SOM, revealing energetic fingerprints long-term changes FYM addition. We compared water-extractable (WEOM) from four experiments with representative WEOM signatures maize, bacterial fungal NM. Long-term increased WEOM, most pronounced polar, unsaturated, oxidised energy-poor compounds. These were linked 2-3-fold bacterial, plant NM signatures. Especially FYM-amended indicated shift dominant use channels. Control showed much lower overlap all NMs, but higher dominance energy-use channels, especially for N-containing A large fraction signals (79% FYM-amended, 94% control soils) was unrelated any three signatures, also mainly responsible nominal oxidation state (NOSC) fertilisation treatments. LC-FT-ICR-MS provided access ~600 novel markers which are readily soluble compositionally distinct classical (ergosterol, aminosugars, etc.). Overall, we highlight insights into contribution by LC-FT-ICR-MS, how it assist constrain compositional impacts on

Language: Английский

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Combined Quantification and Characterization of Dissolved Organic Matter by Liquid Chromatography–Mass Spectrometry Using Charged Aerosol Detection

Journal of the American Society for Mass Spectrometry, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 5, 2024

Dissolved organic matter (DOM) is a complex mixture of thousands molecular formulas comprised an unknown number chemical compounds, the concentration and composition which are critical to ecosystem function biogeochemical cycling. Despite its importance, our understanding DOM lacking. This principally due complexity, means that no single method capable describing in entirety. Quantification typically done by proxy (e.g., relative carbon content) does not necessarily match well compositional data, incomplete analytical windows selectivity different methods. We present integrated liquid chromatography (LC)–diode array detector (DAD)–charged aerosol (CAD)–mass spectrometry (MS) pipeline designed both characterize quantify solid-phase extractable (SPE-DOM) analysis. applied this set eight Swedish water bodies sampled summer winter. Chromophoric SPE-DOM was proportionally higher samples with concentrations but remained relatively consistent between sampling occasions. Ionizable across sites summer. Overall, content very consistently ∼40% These findings suggest at these driven (presumably allochthonous) chromophoric inputs, increased contribution material more ionizable less may be either autochthonous or selectively enriched from allochthonous sources. Thus, minimal additional effort, provided further insights attained any analysis isolation.

Language: Английский

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