The impact of trained immunity in respiratory viral infections DOI
Jocelyne Piret, Guy Boivin

Reviews in Medical Virology, Journal Year: 2024, Volume and Issue: 34(1)

Published: Jan. 1, 2024

Abstract Epidemic peaks of respiratory viruses that co‐circulate during the winter‐spring seasons can be synchronous or asynchronous. The occurrence temporal patterns in epidemics caused by some suggests they could negatively interact with each other. These negative interactions may result from a programme innate immune memory, known as trained immunity, which confer broad protective effects against viruses. It is suggested stimulation cells vaccine pathogen induce their long‐term functional reprogramming through an interplay between metabolic and epigenetic changes, influence transcriptional response to secondary challenge. During coronavirus disease 2019 pandemic, circulation most was prevented non‐pharmacological interventions then resumed at unusual periods once sanitary measures were lifted. With time, should find again own ecological niches. This transition period provides opportunity study population level.

Language: Английский

Comparative analyses of SARS-CoV-2 RNA concentrations in Detroit wastewater quantified with CDC N1, N2, and SC2 assays reveal optimal target for predicting COVID-19 cases DOI

Liang Zhao,

Heidy Peidro Guzman,

Irene Xagoraraki

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 945, P. 174140 - 174140

Published: June 19, 2024

Language: Английский

Citations

5
Detection of hemagglutinin H5 influenza A virus sequence in municipal wastewater solids at wastewater treatment plants with increases in influenza A in spring, 2024 DOI
Marlene K. Wolfe,

Dorothea Duong,

Bridgette Shelden

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 29, 2024

Abstract Prospective influenza A (IAV) RNA monitoring at 190 wastewater treatment plants across the US identified increases in IAV concentrations 59 spring 2024, after typical seasonal period, coincident with identification of highly pathogenic avian (subtype H5N1) circulating dairy cattle US. We developed and validated a hydrolysis-probe RT-PCR assay for quantification H5 hemagglutinin gene. applied it retrospectively to samples from three where springtime were identified. The marker was detected all coinciding increases. Plants located state confirmed outbreaks influenza, H5N1 clade 2.3.4.4b, cattle. Concentrations gene approached overall virus concentrations, suggesting large fraction inputs subtypes. At two plants, industrial discharges containing animal waste, including milk byproducts, permitted discharge into sewers. Our findings demonstrate can detect animal-associated contributions, highlight need consider agricultural wastewater. This work illustrates monitoring’s value comprehensive surveillance diseases zoonotic potential human populations.

Language: Английский

Citations

4
Changes in seasonal respiratory viral infections among pediatric population around the COVID-19 pandemic; 2019–2023 DOI

Mashael Alzaydi,

Aeshah Alosaimi,

Atheer A. Alghamdi

et al.

European Journal of Clinical Microbiology & Infectious Diseases, Journal Year: 2024, Volume and Issue: 43(8), P. 1589 - 1596

Published: May 30, 2024

Language: Английский

Citations

4
Detection and quantification of human immunodeficiency virus-1 (HIV-1) total nucleic acids in wastewater settled solids from two California communities DOI Open Access
Marlene K. Wolfe,

Bridgette Shelden,

Dorothea Duong

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 13, 2024

Abstract Wastewater surveillance for infectious agents has proved useful in identifying circulation of viruses within populations. We investigated the presence and concentration human immunodeficiency virus (HIV)-1 total nucleic acids (including both viral RNA proviral DNA) wastewater solids. retrospectively measured HIV-1 nucleic-acids two samples per week 26 months at treatment plants serving populations with different prevalences HIV infections San Francisco Santa Clara County, California, USA. detected a majority concentrations ranging from non-detect to 3.9×10 5 cp/g (N=459 total). Concentrations were significantly higher plant population prevalence people living than lower prevalence. The amplified primarily DNA thus represented shedding into wastewater. Additionally, we found that nucleic-acid solids orders magnitude those liquid indicating target preferentially sorbs Whether can be used identify numbers incident cases remains unknown. Additional work on individuals viremia is needed translate measurements quantitative information infections.

Language: Английский

Citations

3
Simultaneous detection of influenza A, B and respiratory syncytial virus in wastewater samples by one-step multiplex RT-ddPCR assay DOI Creative Commons

Anastasia Zafeiriadou,

Lazaros Kaltsis,

Νikolaos S. Τhomaidis

et al.

Human Genomics, Journal Year: 2024, Volume and Issue: 18(1)

Published: May 20, 2024

Abstract Background After the occurrence of COVID-19 pandemic, detection other disseminated respiratory viruses using highly sensitive molecular methods was declared essential for monitoring spread health-threatening in communities. The development multiplex assays are simultaneous such even at low concentrations. In present study, a and specific one-step droplet digital PCR (RT-ddPCR) assay developed absolute quantification influenza A (IAV), B (IBV), syncytial virus (RSV), beta-2-microglobulin transcript as an endogenous internal control (IC B2M). Results first evaluated analytical sensitivity specificity, linearity, reproducibility, recovery rates with excellent performance characteristics then applied to 37 wastewater samples previously commercially available in-house quantitative real-time reverse transcription (RT-qPCR) assays. IAV detected 16/37 (43%), IBV 19/37 (51%), RSV 10/37 (27%) samples. Direct comparison RT-qPCR showed statistically significant high agreement (kappa Cohen’s correlation coefficient: 0.834, p = 0.001) (kappa: 0.773, between two assays, while results 0.355, 0.27) good without statistical significance. Conclusions Overall, ddPCR is cost-effective, specific, can simultaneously detect three common complex matrix Due its resistance inhibitors, could be further used early warning system monitoring.

Language: Английский

Citations

3
Estimating epidemic trajectories of SARS-CoV-2 and influenza A virus based on wastewater monitoring and a novel machine learning algorithm DOI
Songzhe Fu, Yixiang Zhang,

Yinghui Li

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 951, P. 175830 - 175830

Published: Aug. 27, 2024

Language: Английский

Citations

3
Respiratory Virus Season Surveillance in the United States Using Wastewater Metrics, 2023–2024 DOI Creative Commons
Elana M. G. Chan, Alexandria B. Boehm

ACS ES&T Water, Journal Year: 2025, Volume and Issue: 5(2), P. 985 - 992

Published: Jan. 22, 2025

Wastewater measurements represent an entire contributing population and can be available within 24 h. Enhanced information about disease occurrence improve health through better timing of policies interventions. We aimed to infer seasonal patterns for common respiratory viruses alongside transmission dynamics SARS-CoV-2 across the USA using wastewater samples. used RNA concentrations influenza A B (IAV/IBV), syncytial virus (RSV), human metapneumovirus (HMPV), from 175 treatment plants (July 2023–June 2024). For IAV, IBV, RSV, HMPV, we determined epidemic onset, offset, peak, duration at national subnational scales. SARS-CoV-2, categorized based on recent levels trends. Epidemic onset occurred in November IAV RSV which aligned with prepandemic norms. Onset January IBV April HMPV were later than expected according historical data. Duration was longer shorter peak dates consistent norms all viruses. Peak coincided high, upward trending concentrations, suggesting potential co-occurrence these

Language: Английский

Citations

0
Wastewater-Based Surveillance of Respiratory Syncytial Virus Reveals a Temporal Disconnect in Disease Trajectory across an Active International Land Border DOI Creative Commons
Mackenzie Beach, Ryland Corchis-Scott, Qiudi Geng

et al.

Environment & Health, Journal Year: 2025, Volume and Issue: 3(4), P. 425 - 435

Published: Jan. 29, 2025

Conventional metrics for tracking infectious diseases, including case and outbreak data syndromic surveillance, can be resource-intensive, misleading, comparatively slow with prolonged collection, analysis authentication. This study examined the 2022–2023 Respiratory Syncytial Virus (RSV) season in a contiguous metropolitan area connected by an active international land border, affording opportunity comparison of respiratory virus spanning two independent public health jurisdictions. Time-lagged cross correlation qualitative examination wastewater signals showed that peak Detroit (MI, USA) RSV predated Windsor (ON, Canada) approximately 5 weeks. A strong positive relationship was observed between N-gene concentrations hospitalization rates Windsor-Essex (Kendall's τ = 0.539, p ≤ 0.001, Spearman's ρ 0.713, 0.001) as well 0.739, 0.888, 0.001). demonstrated surveillance reveal regional differences infection dynamics communities provide measure prevalence RSV, underreported disease. These findings support use cost-effective tool monitoring to enhance existing systems better inform disease mitigation strategies.

Language: Английский

Citations

0
Surveillance via wastewater monitoring and nasal self-collection of specimens (The SWANSS Study): a CFIR-informed qualitative study with key carceral and healthcare stakeholders DOI Creative Commons

Kyler N. Moore,

Patrick M. Bircher,

Emily Awino Ogutu

et al.

BMC Public Health, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 7, 2025

Implementing public health interventions can be challenging in carceral settings. Jails are institutions with the shortest lengths of stay, resulting frequent turnover and constantly shifting populations. had particularly acute challenges during early stages COVID-19 pandemic when incarcerated persons were highly susceptible to infection severe disease. The purpose this study was identify barriers facilitators wastewater-based surveillance (WBS) nasal self-testing (NST) as a combined strategy Fulton County Jail (FCJ), Atlanta, Georgia. Implications for efficient effective infectious disease testing among population relevant ongoing current endemic pathogens future epidemics. We utilized multilevel, theory-informed qualitative approach conduct semi-structured one-on-one small group interviews nine distinct jail stakeholder groups: custody leadership, administrators, officers, maintenance workers, Georgia Department Health's contractor, care leaders, nursing staff, laboratory staff. Interview guides analyses informed by Consolidated Framework Implementation Research (CFIR). used Applied Thematic Analysis domains within CFIR reflecting WBS NST large urban Twelve conducted administrative, healthcare, maintenance, custody, personnel from July 2022 November 2022. Seven constructs identified barriers, sixteen facilitators, one neutral factor implementation NST. stakeholders underscored relative advantage self-testing, highlighted limited resources, expressed concerns sustainability due competing priorities. Many setting that interviewed hierarchical organizational structures, which made decision-making processes regarding complex implement. Given political, structural, factors setting, innovations such require rigorous supported engagement collaboration multitude stakeholders.

Language: Английский

Citations

0
Presence of Potentially Infectious Human Enteric Viruses and Antibiotic Resistance Genes in Mussels from the Campania Region, Italy: Implications for Consumer’s Safety DOI Creative Commons
Iolanda Venuti, Enric Cuevas‐Ferrando, Irene Falcó

et al.

Food and Environmental Virology, Journal Year: 2025, Volume and Issue: 17(2)

Published: May 15, 2025

Language: Английский

Citations

0