Development of a triplex RT-qPCR assay for simultaneous quantification of Japanese encephalitis, Murray Valley encephalitis, and West Nile viruses for environmental surveillance DOI Creative Commons
Yawen Liu, Wendy Smith,

Metasebia Gebrewold

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(10)

Published: Aug. 20, 2024

ABSTRACT The co-circulation of mosquito-borne Japanese encephalitis virus (JEV), Murray Valley (MVEV), and West Nile (WNV) has impacted human animal health in multiple countries worldwide. To facilitate early warnings surveillance the presence these viral infectious agents environment, a triplex reverse transcription-quantitative PCR (RT-qPCR) was developed for simultaneous quantification JEV, MVEV, WNV potential hotspots such as piggery urban wastewater environmental water samples. performance RT-qPCR assay compared with that simplex counterparts, all using same primer probe sequences. quantifiable results showed concordance rate 93.9%–100% (Cohen’s kappa) between assays. mean concentrations exogenous assays were remarkably similar piggery/urban However, impacts matrix effects (i.e., sample composition inhibition) samples on accurate viruses need to be considered. Taken together, this newly will allow more rapid cost-efficient analysis data interpretation. application may valuable tool complement existing disease mosquito approaches used safeguard both humans animals. IMPORTANCE poses significant threats globally. In study, Results demonstrated high sensitivity assays, indicating its efficacy surveillance. This cost-effective offers vital timely monitoring samples, enhancing our ability mitigate outbreaks public health.

Language: Английский

Key considerations for pathogen surveillance in wastewater DOI Creative Commons
Ananda Tiwari, Elena Radu, Norbert Kreuzinger

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 945, P. 173862 - 173862

Published: June 12, 2024

Wastewater surveillance (WWS) has received significant attention as a rapid, sensitive, and cost-effective tool for monitoring various pathogens in community. WWS is employed to assess the spatial temporal trends of diseases identify their early appearances reappearances, well detect novel mutated variants. However, shedding rates vary significantly depending on factors such disease severity, physiology affected individuals, characteristics pathogen. Furthermore, may exhibit differential fate decay kinetics sewerage system. Variable affect detection wastewater. This influence interpretation results conclusions studies. When selecting pathogen WWS, it essential consider it's specific characteristics. If data are not readily available, fate, decay, should be assessed before conducting surveillance. Alternatively, these can compared those similar which available.

Language: Английский

Citations

19
Solid–liquid partitioning of dengue, West Nile, Zika, hepatitis A, influenza A, and SARS-CoV-2 viruses in wastewater from across the USA DOI Creative Commons
Laura Roldan-Hernandez, Camila Van Oost, Alexandria B. Boehm

et al.

Environmental Science Water Research & Technology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Limited information is available on the fate of respiratory and arthropod-borne viruses in wastewater.

Language: Английский

Citations

15
Observations of Respiratory Syncytial Virus (RSV) Nucleic Acids in Wastewater Solids Across the United States in the 2022–2023 Season: Relationships with RSV Infection Positivity and Hospitalization Rates DOI Creative Commons
Alessandro Zulli, Meri R.J. Varkila, Julie Parsonnet

et al.

ACS ES&T Water, Journal Year: 2024, Volume and Issue: 4(4), P. 1657 - 1667

Published: Feb. 29, 2024

Respiratory syncytial virus (RSV) is a leading cause of respiratory illness and hospitalization, but clinical surveillance detects only minority cases. Wastewater could determine the onset extent RSV circulation in absence sensitive case detection, to date, studies wastewater are few. We measured RNA concentrations solids from 176 sites during 2022–2023 season compared those publicly available infection positivity hospitalization rates. Concentrations ranged undetectable 107 copies per gram. concentration aggregated at state national levels correlated with was determined using both rates independent algorithms for 14 states where data were start season. In 4 states, identified same week; 3 preceded onset, 7 occurred after onset. generally peaked week as peaked. Differences peaks versus may reflect inherent differences approaches.

Language: Английский

Citations

13
Detection of dengue virus and chikungunya virus in wastewater in Portugal: an exploratory surveillance study DOI Creative Commons
Sílvia Monteiro,

Raquel Pimenta,

Filipa Nunes

et al.

The Lancet Microbe, Journal Year: 2024, Volume and Issue: unknown, P. 100911 - 100911

Published: Sept. 1, 2024

Language: Английский

Citations

10
A Perspective on Wastewater and Environmental Surveillance as a Public Health Tool for Low- and Middle-Income Countries DOI Creative Commons
Mohammad S. Khan, Christian Wurzbacher, Anna Uchaikina

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 238 - 238

Published: Jan. 22, 2025

Geographical variations in infectious diseases create differences public health priorities between high- and low-income countries. Low- middle-income countries (LMICs) face resource constraints that limit adherence to international monitoring standards for wastewater-based epidemiology (WBE). The development of low-cost WBE programs, such as those detect SARS-CoV-2, offers LMICs a promising tool pathogens local concern. In this work, we summarize important wastewater biomarkers their associated challenges, ranging from causing gastroenteritis putative markers plant linked food safety, well antimicrobial resistance. We raise awareness the great potential highlight critical markers, research needs, strategies necessary establish tailored surveillance programs.

Language: Английский

Citations

1
Environmental Surveillance of Vector-Borne Diseases in a Non-Sewered System: A Case Study in Mozambique DOI
Sílvia Monteiro, Filipa Nunes, Michaque Dosse

et al.

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 14, 2025

Arboviral diseases pose major economic and social threats in less economically developed countries (LEDCs), where monitoring is challenging, especially rapidly growing cities with informal settlements. In this study, we aimed to explore environmental surveillance (ES) a non-sewered setting as complement syndromic Maputo, Mozambique. Water samples were collected from nine points along the Infulene River (n = 66) Mozambique February September 2023. The presence of arboviruses (Dengue (DENV), Chikungunya (CHIKV), West Nile (WNV), Usutu (USUV) virus) was determined by RT-qPCR. For specific detection CHIKV, two RT-qPCR assays used: Nsp1, targeting non-structural protein 1 gene (nsP1) E1, E1 envelope (E1). DENV detected 82% (54/66) samples, median viral RNA load 2.7 × 10-2 (2.2 105 copies/L (cp/L)), while CHIKV detectable 98% (65/66) 4.8 cp/L) for nsP1 8.0 (4.8 cp/L), USUV 6% (4/66) at 4.1 10-7 (0 cP/L), positive varying between 1.8 10-3 (7.1 102 4.95 (2.1 103 cp/L). WNV not throughout study. prevalence concentration varied across sampling dates. Our study demonstrated potential ES tool assessing circulation Mozambique, sewered system unavailable. Consequently, could be expanded polio include other targets LEDCs.

Language: Английский

Citations

1
Prospective study of Candida auris nucleic acids in wastewater solids in 190 wastewater treatment plants in the United States suggests widespread occurrence DOI Creative Commons
Alessandro Zulli, Elana M. G. Chan,

Bridgette Shelden

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(8)

Published: July 23, 2024

is an emerging, multidrug-resistant fungal pathogen that poses a significant public health threat in healthcare settings. Despite yearly clinical cases rapidly increasing from 77 to 8,131 the last decade, surveillance data on its distribution and prevalence remain limited. We implemented novel assay for

Language: Английский

Citations

6
Strengthening arbovirus surveillance: bridging gaps for global health preparedness DOI

Eleonora Cella,

Mónica V. Cunha, Luíz Carlos Júnior Alcântara

et al.

Trends in Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Wastewater Metavirome Diversity: Exploring Replicate Inconsistencies and Bioinformatic Tool Disparities DOI Open Access
Anderson Fragoso dos Santos, Mónica Nunes, Andreia Filipa-Silva

et al.

International Journal of Environmental Research and Public Health, Journal Year: 2025, Volume and Issue: 22(5), P. 707 - 707

Published: April 30, 2025

This study investigates viral composition in wastewater through metagenomic analysis, evaluating the performance of four bioinformatic tools—Genome Detective, CZ.ID, INSaFLU-TELEVIR and Trimmomatic + Kraken2—on samples collected from sites each two treatment plants (WWTPs) Lisbon, Portugal April 2019. From site, we processed separately three replicates one pool nucleic acids extracted replicates. A total 32 were using sequence-independent single-primer amplification (SISPA) sequenced on an Illumina MiSeq platform. Across 128 sample–tool combinations, read counts varied widely, 3 to 288,464. There was a lack consistency between their pools terms abundance diversity, revealing heterogeneity matrix variability sequencing effort. also difference software tools highlighting impact tool selection community profiling. positive correlation crAssphage human pathogens found, supporting as proxy for public health surveillance. custom Python pipeline automated identification report processing, taxonomic assignments diversity calculations, streamlining analysis ensuring reproducibility. These findings emphasize importance depth, standardized pipelines advancing wastewater-based epidemiology.

Language: Английский

Citations

0
Tiled amplicon sequencing of monkeypox virus from wastewater: a novel approach for clade and subclade level differentiation DOI

Mathew Fisher,

Jennifer Ali,

Shayna Giesbrecht

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: May 5, 2025

Abstract Wastewater-based surveillance (WBS) has modernized in recent years and emerged as an important tool for the monitoring of viral pathogens, including monkeypox virus (MPXV). Here we describe a novel targeted amplicon sequencing method developed clade subclade characterization MPXV from municipal wastewater. This new addresses limitations PCR-based methods challenges pathogen displaying low load wastewater samples. A tiled scheme composed 11 primer pairs targeting 4.2 kb portion inverted terminal repeat (ITR) region genome was designed tested. In silico analysis demonstrated high accuracy calls using full target region, with specific amplicons also exhibiting strong performance individually. An consensus sequence representing entire successfully sequenced sample differentiated positive controls by distinct deletion within short homopolymeric region. Notably, clade-informing data achieved partial sequences recovered lower abundance study presents enhanced genomic resolution compared to existing approaches, providing critical genomic-level information informing public health interventions.

Language: Английский

Citations

0