Nanoscience & Nanotechnology-Asia,
Journal Year:
2024,
Volume and Issue:
14(4)
Published: June 19, 2024
Introduction:
Nanoparticles
are
used
in
industrial
products,
such
as
textiles,
to
induce
novel
properties,
antibacterial,
antistatic,
UV
blocking,
self-cleaning
wrinkle
resistance,
and
water
oil
repellent.
Moreover,
using
enzymes
(protease,
lipase,
amylase,
cellulase)
is
widespread
detergent
industries
for
washing
conditions.
Methods:
This
research
examines
the
interactions
between
metal
(Ag)
oxide
nanoparticles
(TiO2
ZnO
NPs)
cellulase,
protease,
lipase
their
impacts
on
enzyme
activity.
Using
a
central
composite
design,
total
of
320
experiments
under
different
conditions
were
conducted
determine
extent
change
Results
indicated
that
had
lowest
activity
interaction
with
silver
nanoparticles,
while
cellulase
protease
most
affected
by
Ag
NPs
a-TiO2.
Results:
The
surface
response
examined
parameters
showed
effect
from
time
temperature
enzyme/nanoparticle
ratio
parameters.
result
demonstrated
physical,
chemical,
biological
differences
existed
nanoparticle
interface.
Conclusion:
findings
can
be
improve
conditions,
aiming
retain
traits.
ACS Omega,
Journal Year:
2024,
Volume and Issue:
9(47), P. 46698 - 46732
Published: Nov. 14, 2024
Immobilization
of
lipases
by
physical
adsorption
improves
their
stability,
recovery,
and
reusability
in
biotechnological
processes.
The
present
review
provides
an
advanced
bibliometric
analysis
a
comprehensive
overview
research
progress
this
field.
By
searching
Web
Science,
39,575
publications
were
analyzed,
325
relevant
articles
selected.
Key
journals,
countries,
institutions,
authors
identified.
most
cited
focus
on
biofuel
production
industrial
applications.
revealed
four
themes
with
the
biofuel.
method
is
effective
when
appropriate
support
used.
Despite
decrease
patent
applications,
interest
remains
high.
Future
studies
should
optimizing
materials
exploring
new
applications
technique.
detailed
understanding
immobilization
adsorption.
Nanomaterials,
Journal Year:
2024,
Volume and Issue:
14(8), P. 700 - 700
Published: April 17, 2024
Here,
we
developed
magnetically
recoverable
biocatalysts
based
on
magnetite
nanoparticles
coated
with
an
ultra-thin
layer
(about
0.9
nm)
of
chitosan
(CS)
ionically
cross-linked
by
sodium
tripolyphosphate
(TPP).
Excessive
CS
amounts
were
removed
multiple
washings
combined
magnetic
separation.
Glucose
oxidase
(GOx)
was
attached
to
the
support
via
interaction
N-hydroxysuccinimide
(NHS)
in
presence
carbodiimide
(EDC)
leading
a
covalent
amide
bond.
These
steps
result
formation
biocatalyst
for
D-glucose
oxidation
D-gluconic
acid
be
used
preparation
pharmaceuticals
due
benign
character
components.
To
choose
catalyst
best
catalytic
performance,
CS,
TPP,
NHS,
EDC,
and
GOx
varied.
The
optimal
allowed
100%
relative
activity.
immobilization
prevents
loss
allows
repeated
use.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(5), P. 2746 - 2746
Published: Feb. 27, 2024
Multi-enzymatic
strategies
have
shown
improvement
in
bioconversion
during
cofactor
regeneration.
In
this
study,
purified
l-arabinitol
4-dehydrogenase
(LAD)
and
nicotinamide
adenine
dinucleotide
oxidase
(Nox)
were
immobilized
via
individual,
mixed,
sequential
co-immobilization
approaches
on
magnetic
nanoparticles,
evaluated
to
enhance
the
conversion
of
l-xylulose.
Initially,
immobilization
LAD
or
Nox
nanoparticles
resulted
a
maximum
yield
relative
activity
91.4%
98.8%,
respectively.
The
enzymes
showed
better
pH
temperature
profiles
than
corresponding
free
enzymes.
Furthermore,
these
mixed
methods
high
loadings
114
122
mg/g
support,
Sequential
proved
more
beneficial
for
higher
because
retaining
residual
activity.
Sequentially
co-immobilized
with
broader
pH,
temperature,
storage
stability
controls,
along
reusability.
To
best
our
knowledge,
is
first
report
l-xylulose
production.
This
finding
suggests
that
selecting
strategy
using
individual
enhancing
applications.
ACS Omega,
Journal Year:
2024,
Volume and Issue:
9(23), P. 24558 - 24573
Published: May 29, 2024
Recently,
hybrid
nanoflowers
(hNFs),
which
are
accepted
as
popular
carrier
supports
in
the
development
of
enzyme
immobilization
strategies,
have
attracted
much
attention.
In
this
study,
horseradish
peroxidase
(HRP)
was
immobilized
to
mesoporous
magnetic
Fe3O4–NH2
by
forming
Schiff
base
compounds
and
HRP@Fe3O4–NH2/hNFs
were
then
synthesized.
Under
optimal
conditions,
95.0%
available
HRP
on
Fe3O4–NH2/hNFs.
Structural
morphology
characterization
synthesized
investigated.
The
results
demonstrated
that
average
size
determined
be
around
220
nm.
ζ-potential
saturation
values
−33.58
mV
∼30
emu/g,
respectively.
Additionally,
optimum
pH,
temperature,
thermal
stability,
kinetic
parameters,
reusability,
storage
stability
examined.
It
observed
pH
value
shifted
from
5.0
8.0
after
immobilization,
while
temperature
30
80
°C.
Km
calculated
15.5502
7.6707
mM
for
free
HRP@Fe3O4–NH2/hNFs,
respectively,
Vmax
0.0701
0.0038
min–1.
low
indicated
affinity
its
substrate
increased.
showed
higher
than
HRP,
residual
activity
six
usage
cycles
approximately
45%.
While
lost
all
within
120
min
at
65
°C,
retained
almost
during
6
h
incubation
period
Most
importantly,
good
potential
efficiency
biodegradation
methyl
orange,
phenol
red,
methylene
blue
dyes.
used
a
total
8
degrade
blue,
degradation
81,
96,
56%
obtained
h,
Overall,
we
believe
reported
work
can
potentially
various
industrial
environmental
applications,
particularly
recalcitrant
compounds,
such
textile
EPL (Europhysics Letters),
Journal Year:
2024,
Volume and Issue:
147(2), P. 21002 - 21002
Published: July 1, 2024
Abstract
Chemical
affinities
are
responsible
for
driving
active
matter
systems
out
of
equilibrium.
At
the
nano-scale,
molecular
machines
interact
with
surrounding
environment
and
subjected
to
external
forces.
The
mechano-chemical
coupling
which
arises
naturally
in
these
reveals
a
complex
interplay
between
chemical
mechanical
degrees
freedom
strong
impact
on
their
mechanism.
By
considering
various
models
far
from
equilibrium,
we
show
that
tuning
applied
forces
gives
rise
nonlinear
response
causes
non-monotonic
behaviour
machines’
activity.
Our
findings
have
implications
understanding,
designing,
triggering
such
processes
by
controlled
application
fields,
including
collective
dynamics
larger
non-equilibrium
where
total
dissipation
performance
might
be
affected
internal
inter-particle
interactions.
