Anti-Inflammatory and Wound Healing Properties of Leaf and Rhizome Extracts from the Medicinal Plant Peucedanum ostruthium (L.) W. D. J. Koch

Molecules, Journal Year: 2022, Volume and Issue: 27(13), P. 4271 - 4271

Published: July 2, 2022

Peucedanum ostruthium (L.) W. D. J. Koch (Apiaceae) is a worldwide perennial herb native to the mountains of central Southern Europe. The rhizome has long tradition in popular medicine, while ethnobotanical surveys have revealed local uses leaves for superficial injuries. To experimentally validate these uses, plant material was collected Gran Paradiso National Park, Aosta Valley, Italy, and were micromorphologically phytochemically characterized. Polyphenol-enriched hydroalcoholic leaf extracts, used cell-free assays, showed strong concentration-dependent antioxidant anti-inflammatory activities. In vitro tests cyclooxygenase lipoxygenase inhibition by extract, extract induced only inhibition. MTT assays on HaCaT keratinocytes L929 fibroblasts low cytotoxicity extracts. scratch wound test resulted induction closure with effect lower. same cells similar both results confirmed traditional medicinal as an healing remedy injuries but also highlighted that can be exploited purposes equal or superior effectiveness.

Language: Английский

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Exploring the Antioxidant Properties of Caffeoylquinic and Feruloylquinic Acids: A Computational Study on Hydroperoxyl Radical Scavenging and Xanthine Oxidase Inhibition

Antioxidants, Journal Year: 2023, Volume and Issue: 12(9), P. 1669 - 1669

Published: Aug. 25, 2023

Caffeoylquinic (5-CQA) and feruloylquinic (5-FQA) acids, found in coffee other plant sources, are known to exhibit diverse biological activities, including potential antioxidant effects. However, the underlying mechanisms of these phenolic compounds remain elusive. This paper investigates capacity mode action 5-CQA 5-FQA as natural antioxidants acting hydroperoxyl radical scavengers xanthine oxidase (XO) inhibitors. The scavenging was investigated using thermodynamic kinetic calculations based on DFT method, taking into account influence physiological conditions. Blind docking molecular dynamics simulations were used investigate inhibition toward XO enzyme. results showed that potent both polar lipidic media, with rate constants higher than those common antioxidants, such Trolox BHT. carrying catechol moiety be more environments. Furthermore, show good affinity active site enzyme form stable complexes. hydrogen atom transfer (HAT) mechanism exclusive lipid while HAT SET (single electron transfer) possible water. may, therefore, considered health benefits.

Language: Английский

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Exploring the Phytochemical Composition and the Bioactive Properties of Malbec and Torrontés Wine Pomace from the Calchaquíes Valleys (Argentina) for their Sustainable Exploitation

Published: May 29, 2024

Hydroalcoholic extracts from Malbec and Torrontés wine pomace (Vitis vinifera L.) originating the high-altitude vineyards of Argentina's Calchaquí Valleys were characterized. Total phenolics, hydroxycinnamic acids, orthodiphenols, anthocyanins, non-flavonoid total flavonoids, flavones/flavonols, flavanones/dihydroflavonols, tannins quanti-fied through spectrophotometric methods, with extract exhibiting higher concentra-tions in most phytochemical groups when compared to Torrontés. HPLC-DAD identified more than 30 phenolic compounds both extracts. displayed superior antiradical ac-tivity (ABTS cation, nitric oxide, superoxide anion radicals), reduction power (iron, copper phosphomolybdenum), hypochlorite scavenging, iron chelating ability, compara-tively The cytotoxicity assessments revealed that affected viability HT29-MTX Caco-2 colon cancer cells by 70% 50%, respectively, at highest tested concentration (1 mg/mL). At same time, did not demonstrate acute toxicity Artemia salina or red blood cell assays 500 µg/mL. Both inhibited lipoxygen-ase enzyme, also reducing tyrosinase activity neither xan-thine oxidase. substantial content diverse biological activities Valleys' pomaces underline their potentialities be valorized for pharmaceutical, cosmetic, food industries.

Language: Английский

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Green Biosynthesis of Silver Nanoparticles Using Aqueous Extracts of Ageratum Conyzoides and Their Anti-Inflammatory Effects

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: unknown

Published: March 7, 2023

The NLRP3 inflammasome, which plays a central role in innate immunity, is linked to variety of inflammatory diseases, and thus it may provide new target for the treatment those diseases. Biosynthesized silver nanoparticles (AgNPs), particularly synthesized using medicinal plant extracts, have recently been shown be promising therapeutic option. Herein, aqueous extract Ageratum conyzoids was used prepare series sized AgNPs (AC-AgNPs), smallest mean particle size 30 ± 1.3 nm with polydispersity 0.328 0.009. ζ potential value −28.77 mobility −1.95 0.24 cm2/(v·s). Its main ingredient, elemental silver, accounted about 32.71 4.87% its mass, other ingredients included amentoflavone-7,7⁗-dimethyl ether, 1,3,5-tricaffeoylquinic acid, kaempferol 3,7,4′-triglucoside, 5,6,7,3′,4′,5′-hexamethoxyflavone, kaempferol, ageconyflavone B. In LPS+ATP-stimulated RAW 264.7 THP-1 cells, AC-AgNPs significantly inhibited release IL-1β, IL-18, TNF-α, caspase-1, indicating that can inhibit activation inflammasome. mechanistic study revealed could decrease phosphorylation levels IκB-α p65, resulting decreased expression inflammasome-related proteins, including pro-IL-1β, procaspase 1, caspase 1P20, NLRP3, ASC, also scavenge level intracellular ROS prevent inflammasome assembly. Furthermore, attenuated vivo cytokines by suppressing peritonitis mouse model. Our provides evidence as-prepared process might treat inflammasome-driven

Language: Английский

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The Action Mechanisms, Anti-Cancer and Antibiotic-Modulation Potential of Vaccinium myrtillus L. Extract

Discovery Medicine, Journal Year: 2023, Volume and Issue: 35(177), P. 590 - 590

Published: Jan. 1, 2023

Herbal medicinal products containing Vaccinium myrtillus L. (bilberry) fruits and fruit extracts are widely available in the market. Although bilberry leaves stems considered as bio-waste, they contain much higher levels of phenolic compounds than fruits. The study aimed to investigate antimicrobial anticancer potential aerial part from (V. myrtillus, VM) plants harvested at high altitudes Armenian landscape characterize bioactive phytochemicals.For evaluation antioxidant properties, chemical-based tests (total flavonoid content, antiradical activity 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) 2,2-diphenyl-1-picrylhydrazyl (DPPH) tests) cellular (CAA) assay were applied. Genotoxicity properties extract alone combination with fluorouracil explored human cancer normal cell lines. Antibacterial V.myrtillus antibiotics, well their effect on proton-flux rate through membrane bacterial strains. characterization active phytochemicals was done using Liquid Chromatography-Quadrupole-Orbitrap High-Resolution Mass Spectrometry (LC-Q-Orbitrap HRMS).The V. demonstrated promising all tests. selective cytotoxic documented against various lines (human colon adenocarcinoma (HT29), breast (MCF-7) cervical carcinoma (HeLa)), while it did not inhibit growth tested primary renal mixed epithelial cells (HREC) even 10-fold concentrations. have genotoxic comet making a source for development preparations. investigated directly Escherichia coli (E. coli) Salmonella typhimurium (S. typhimurium) strains up 1 mg/mL concentration. However, enhanced kanamycin intake increased its efficiency E. strain. phytochemical showed presence different groups phenolics.Based obtained data, we suggest parts plant an alternative natural food supplements, nutraceuticals, functional foods medicine.

Language: Английский

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Establishment and elicitation of liquid adventitious root cultures of Inula crithmoides L. for increased caffeoylquinic acids production and hepatoprotective properties

Plant Cell Tissue and Organ Culture (PCTOC), Journal Year: 2024, Volume and Issue: 156(2)

Published: Jan. 20, 2024

Language: Английский

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Antioxidant, Anti-Diabetic, and Anti-Inflammation Activity of Garcinia livingstonei Aqueous Leaf Extract: A Preliminary Study

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3184 - 3184

Published: March 10, 2024

Diabetes mellitus (DM) is the second leading cause of mortality globally. The increased concern for DM due to underlying complications accompanying hyperglycaemia, associated with oxidative stress and consequent inflammation. investigation safe effective treatments necessary. In present study, cytotoxicity, phytochemical analysis, antioxidant capacity, anti-inflammatory, antidiabetic effects in an aqueous extract Garcinia livingstonei leaves were assessed. All tested concentrations showed no toxicity against C3A hepatocytes. Several phenolic compounds identified using ultra-high performance liquid chromatography mass spectrometry (UHPLC-MS). total polyphenol content was 100.9741 mg GAE/g, 16.7712 CE/g flavanols, 2.3548 QE/g flavonols. capacity values 253.4268 AAE/g, 192.232 TE/g, 167.8724 TE/g ferric reducing power (FRAP), Trolox equivalent (TEAC), 2,2-diphenyl-1-pycrylhydrazyl (DPPH), respectively. plant significantly (p < 0.05) demonstrated anti-inflammatory hypoglycaemic a dose-dependent manner, α-glucosidase inhibition being higher than standard conventional drug (acarbose). findings this study revealed potential constituents G. leaf treatment. Further studies on preparation mechanisms action treatment are recommended.

Language: Английский

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A Comprehensive View on the Impact of Chlorogenic Acids on Colorectal Cancer

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(7), P. 6783 - 6804

Published: July 2, 2024

Chlorogenic acids are plant secondary metabolites, chemically-polyphenols with similar biological activity, formed through the esterification of quinic acid and hydrocinnamic moieties. They best known for their high concentration in coffee other dietary sources antioxidant properties that they exhibit. Both chlorogenic extracts containing significant amounts compounds show promising vitro activity against colorectal cancer. With being most popular drink world, cancer at an unfortunate peak incidence mortality, mechanisms which anti-tumorigenic effect could be functionalized CRC prevention seem appealing to study. Therefore, this review aims enable a better understanding modes action combating carcinogenesis, focus on cell cycle arrest, induction apoptosis, modulation Wnt, Pi3K/Akt, MAPK signal transduction pathways, alongside reduction number inflammatory cytokines chemokines counterintuitive beneficial elevation oxidative stress.

Language: Английский

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Engineering the Rhizosphere Microbiome with Plant Growth Promoting Bacteria for Modulation of the Plant Metabolome

Plants, Journal Year: 2024, Volume and Issue: 13(16), P. 2309 - 2309

Published: Aug. 20, 2024

Plant-growth-promoting bacteria (PGPB) have beneficial effects on plants. They can promote growth and enhance plant defense against abiotic stress disease, these are associated with changes in the metabolite profile. The research problem addressed this study was impact of inoculation PGPB profile

Language: Английский

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De Novo synthesis of selenium-doped CeO2@Fe3O4 nanoparticles for improving secondary metabolite biosynthesis in Carthamus tinctorius cell suspension culture

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 159705 - 159705

Published: Jan. 1, 2025

Language: Английский

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