For
a
significant
period,
conventional
breeding
and
genetic
modification
were
the
key
techniques
that
effective
in
managing
biotic
abiotic
stresses
crops
adding
desirable
traits.
However,
recent
appearance
of
novel
diseases
unexpected
climatic
changes
have
substantial
implications
for
agriculture
worldwide
urged
scientists
to
look
alternative
methods
quickly
manage
seasonal
crises.
The
primary
emphasis
this
chapter
is
on
obstacles
diverse
biotechnological
employed
enhance
crop
resilience
against
range
stressors
plants.
Furthermore,
we
consider
gene
transformation,
omics
techniques,
genome
editing,
other
sophisticated
tools
utilize
transcriptomic,
proteomic,
metabolomic,
phenomics,
RNA
interference,
epigenome
modifications
enhancing
plant
resilience.
Lastly,
examine
potential
merging
these
eco-friendly
innovative
with
promote
modern
aid
tolerance
different
biotic,
growth
promotion.
Cells,
Journal Year:
2022,
Volume and Issue:
11(17), P. 2665 - 2665
Published: Aug. 27, 2022
The
Clustered
Regularly
Interspaced
Short
Palindromic
Repeats
(CRISPR)/CRISPR-associated
(Cas)
system
is
widely
used
as
a
genome-editing
tool
in
various
organisms,
including
plants,
to
elucidate
the
fundamental
understanding
of
gene
function,
disease
diagnostics,
and
crop
improvement.
Among
CRISPR/Cas
systems,
Cas9
one
nucleases
for
DNA
modifications,
but
manipulation
RNA
at
post-transcriptional
level
limited.
recently
identified
type
VI
systems
provide
platform
precise
without
permanent
changes
genome.
Several
studies
reported
efficient
application
Cas13
studies,
such
viral
interference,
knockdown,
detection
organisms.
was
also
produce
virus
resistance
most
plant
viruses
are
viruses.
However,
CRISPR/Cas13
biology
still
its
infancy.
This
review
discusses
current
prospective
applications
CRISPR/Cas13-based
editing
technologies
plants.
Journal of genetics and genomics/Journal of Genetics and Genomics,
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 1, 2024
Sorghum,
renowned
for
its
substantial
biomass
production
and
remarkable
tolerance
to
various
stresses,
possesses
extensive
gene
resources
phenotypic
variations.
A
comprehensive
understanding
of
the
genetic
basis
underlying
complex
agronomic
traits
is
essential
unlocking
potential
sorghum
in
addressing
food
feed
security
utilizing
marginal
lands.
In
this
context,
we
provide
an
overview
major
trends
genomic
resource
studies
focusing
on
key
over
past
decade,
accompanied
by
a
summary
functional
platforms.
We
also
delve
into
molecular
functions
regulatory
networks
impactful
genes
important
agricultural
traits.
Lastly,
discuss
synthesize
current
challenges
prospects
advancing
design
breeding
gene-editing
polymerization
excellent
alleles,
with
aim
accelerating
development
desired
varieties.
Chemical and Biological Technologies in Agriculture,
Journal Year:
2025,
Volume and Issue:
12(1)
Published: Feb. 10, 2025
Abstract
Histone
acetylation
is
a
key
epigenetic
modification
involved
in
plant
development.
Although
histone
deacetylase
inhibitors
(HDACi)
are
commonly
studied
human
diseases,
their
role
regulating
deacetylation
plants
remains
unclear.
This
study
explores
the
function
of
Citronellol,
volatile
small
molecule,
as
plant-derived
HDACi
using
Arabidopsis
thaliana
(L.)
Heynh
(
A.
thaliana)
model.
Citronellol
at
concentrations
3
and
6
mM
enhanced
both
root
development
aboveground
growth.
Enzyme
activity
assays,
molecular
docking,
dynamics
simulations
showed
that
binds
to
specific
residues
(PHE:64,
ARG:65,
MET:1,
ILE:214)
AtSRT1
Arabidopsis,
inhibiting
its
elevating
H3K9ac
levels.
Integrated
RNA-seq
ChIP-seq
analyses
revealed
increased
expression
genes
linked
growth
development,
including
ATCTH
,
CPL3
IBR5
TCP4
KUA1
through
activation
hormone
signaling
pathways.
These
findings
provide
new
insights
into
regulation
by
identifying
it
novel
HDACi.
could
serve
an
effective
regulator,
offering
valuable
applications
for
agricultural
Graphical
Abstract
Evolutionarily,
polyploidy
represents
a
smart
method
for
adjusting
agronomically
important
in
crops
through
impacts
on
genomic
abundance
and
chromatin
condensation.
Autopolyploids
have
relatively
concise
genetic
background
with
great
diversity
provide
an
ideal
system
to
understand
epigenetic
mechanisms
attributed
the
genome-dosage
effect.
However,
whether
how
genome
duplication
events
during
autopolyploidization
impact
signatures
are
less
understood
crops.
To
address
it,
we
generated
autotetraploid
rice
line
from
diploid
progenitor,
Oryza
sativa
ssp.
indica
93-11.
Using
transposase-accessible
sequencing,
found
that
autopolyploids
lead
higher
number
of
accessible
regions
(ACRs)
euchromatin,
most
which
encode
protein-coding
genes.
As
expected,
profiling
ACR
densities
supported
effect
ACRs
transcriptional
gene
activities
relies
their
positions
genome,
regardless
doubling.
noticed
favors
genic
as
main
drivers
changes.
In
addition,
probed
intricate
crosstalk
among
various
kinds
marks
expression
patterns
ACR-associated
both
plants
by
integrating
multiple-omics
analyses,
including
immunoprecipitation
sequencing
RNA-seq.
Our
data
suggested
combination
H3K36me2
H3K36me3
may
be
associated
dynamic
perturbation
introduced
autopolyploidization.
consequence,
numerous
metabolites
were
stimulated
Collectively,
our
findings
suggest
autotetraploids
reshape
morphology
products
modulating
profiling,
resulting
pragmatic
means
crop
improvement.
Journal of Agricultural and Food Chemistry,
Journal Year:
2023,
Volume and Issue:
71(14), P. 5836 - 5850
Published: March 30, 2023
Sorghum
seed
germination
is
accompanied
by
increases
in
nutrient
contents
and
reduced
levels
of
antinutrients
therefore
being
applied
to
food
processing.
However,
the
characterization
acetylated
histone
H3
at
lysine
residue
9
(H3K9ac)
sorghum
postgermination
has
lagged.
In
this
study,
we
performed
chromatin
immunoprecipitation
sequencing
(ChIP-seq)
identify
H3K9ac
enrichment
obtained
transcriptome
stages.
More
than
10,000
hypoacetylated
genes
gained
marks
addition,
observed
that
expression
main
deacetylase
(HDAC)
was
elevated.
The
application
HDAC
inhibitor
trichostatin
A
(TSA)
resulted
growth
arrest,
suggesting
repression
modification
critical
for
postgermination.
Additionally,
a
comprehensive
view
abundant
genomic
changes
H3K9ac-marked
regions
transcription
between
mock
TSA
treatment
groups,
which
suggested
required
late
stage
autotrophic
seedling
establishment.
Metabolic
profiling,
analyses,
ChIP-seq
revealed
enriched
involved
phenylpropanoid,
including
lignin
flavonoid,
biosynthesis.
Our
results
suggest
important
roles
