The role of arabinoxylan purified from wheat bran in bread and biscuits: Impacts on digestibility and colonic fermentability in vitro

LWT, Journal Year: 2024, Volume and Issue: 203, P. 116314 - 116314

Published: June 14, 2024

In this study, arabinoxylan (AX) isolated from wheat bran was used to prepare AX-fortified bread and biscuits, the effects of amount method AX addition on nutritional properties baked products were investigated through simulated digestibility colonic fermentability analysis in vitro. The purified demonstrated a high total sugar content (92.75%), molecular weight (Mw) (669.8 kDa), low polymer dispersity index (PDI) arabinose xylose (A/X) ratio (1.21). vitro test indicated that could dose-dependently inhibit starch both biscuits. Most importantly, it also transfer more protein into colon gastrointestinal tract, leading changes chemical composition food residues. Results showed alterations residue affected its fermentability: relative carbohydrate enhance prebiotic effect. This study provides valuable insights role dietary fibres foods, aiming achieve precise nutrition for high-fibre products.

Language: Английский

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Probiotic treatment induces sex-dependent neuroprotection and gut microbiome shifts after traumatic brain injury

Journal of Neuroinflammation, Journal Year: 2025, Volume and Issue: 22(1)

Published: April 20, 2025

Abstract Background Recent studies have highlighted the potential influence of gut dysbiosis on traumatic brain injury (TBI) outcomes. Alterations in abundance and diversity Lactobacillus species may affect immune dysregulation, neuroinflammatory responses, anxiety- depressive-like behaviors, neuroprotective mechanisms activated response to TBI. Objective This study aims evaluate protective preventive effects Pan-probiotic (PP) treatment inflammatory during both acute chronic phases Methods Males female mice underwent controlled cortical impact (CCI) or sham. They received a PP mixture drinking water containing strains plantarum , L. reuteri helveticas fermentum rhamnosus gasseri casei. In group, vehicle (VH) for 7 weeks before TBI, continuing until 3 days post-injury (dpi). began 2 TBI was extended through 35 dpi. The taxonomic microbiome profiles fecal samples were evaluated using 16S rRNA V1-V3 sequencing analysis, Short-chain fatty acids (SCFAs) measured. Immunohistochemical, situ hybridization, histological analyses performed assess neuroinflammation post-TBI, while behavioral assessments conducted sensorimotor cognitive functions. Results Our findings suggest that 7-week administration induces specific microbial changes, including increased beneficial bacteria such as Lactobacillaceae Limosilactobacillus Lactiplantibacillus . reduces lesion volume cell death at dpi, elevates SCFA levels decreases microglial activation time points, particularly males. Additionally, improved motor recovery males alleviated behaviors females. Conclusion indicate modulates composition, neuroinflammation, improves deficits following with these being pronounced male mice.

Language: Английский

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Physicochemical properties and bioactivity of polysaccharides from Isaria cicadae Miquel with different extraction processes: effects on gut microbiota and immune response in mice

Food & Function, Journal Year: 2022, Volume and Issue: 13(18), P. 9268 - 9284

Published: Jan. 1, 2022

The effect of different extraction processes on the physicochemical characterization, digestibility, antioxidant activity and prebiotic Isaria cicadae Miquel (ICM) fruiting body polysaccharides was studied. Furthermore, ultrasound-assisted ICM (U-ICM) gut microbiota, intestinal barrier immune response deeply explored. This study found that ICMs showed high indigestibility in both α-amylase artificial gastric juice, indicating have potential as dietary fiber. In contrast, U-ICM had best potential. Meanwhile, there a structure-activity relationship between content uronic acid, arabinose galactose. When healthy mice were fed for 42 days, relative abundances Lactobacillus, Akkermansia, Bacteroides to increase significantly, while Clostridium decreased significantly. significantly promotes expression tight junction protein production cytokines, function enhancing regulating host response. conclusion, fiber has be developed health-promoting component or functional food. research provided valuable resource further exploring ICMs.

Language: Английский

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Lactiplantibacillus plantarum CCFM8661 alleviates d-galactose-induced brain aging in mice by the regulation of the gut microbiota

Food & Function, Journal Year: 2023, Volume and Issue: 14(22), P. 10135 - 10150

Published: Jan. 1, 2023

Lactiplantibacillus plantarum CCFM8661 alleviates D-galactose induced aging in mice.

Language: Английский

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Pectin as a biofunctional food: comprehensive overview of its therapeutic effects and antidiabetic-associated mechanisms

Deleted Journal, Journal Year: 2024, Volume and Issue: 6(6)

Published: May 29, 2024

Abstract Pectin is a complex polysaccharide found in variety of fruits and vegetables. It has been shown to have potential antidiabetic activity along with other biological activities, including cholesterol-lowering properties, antioxidant activity, anti-inflammatory immune-modulatory effects, augmented healing diabetic foot ulcers health benefits. There are several pectin-associated mechanisms, such as the regulation glucose metabolism, reduction oxidative stress, increased insulin sensitivity, appetite suppression modulation gut microbiome. Studies that pectin supplementation effects different animal models vitro. In human studies, positive effect on blood control, particularly individuals type 2 diabetes. also shows synergistic by enhancing potency efficacy drugs when taken together. conclusion, be an effective agent. However, further research needed fully understand its detailed molecular mechanisms various models, functional food formulations safety profiles for treatment management diabetes associated complications humans. The current study was carried out provide critical approach towards therapeutical potential, anti-diabetic underlying basis existing knowledge.

Language: Английский

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Differences in the Ability of Lactic Acid Bacteria To Prevent Acute Alcohol-Induced Liver Injury via the Gut Microbiota–Bile Acid–Liver Axis

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(27), P. 15265 - 15275

Published: June 25, 2024

Probiotics can regulate gut microbiota and protect against acute alcohol-induced liver injury through the gut-liver axis. However, efficacy is strain-dependent, their mechanism remains unclear. This study investigated effect of lactic acid bacteria (LAB), including

Language: Английский

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The galactomannan-EGCG physical complex: Effect of branching degree and molecular weight on structural and physiological properties

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 343, P. 122447 - 122447

Published: June 28, 2024

Language: Английский

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Maternal intervention with a combination of galacto-oligosaccharides and hyocholic acids during late gestation and lactation increased the reproductive performance, colostrum composition, antioxidant and altered intestinal microflora in sows

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: June 12, 2024

Introduction This study was conducted to evaluate the effects of dietary galacto-oligosaccharides (GOS) and hyocholic acids (HCA) during late gestation lactation on reproductive performance, colostrum quality, antioxidant capacity gut microbiota in multiparous sows. Methods A total 60 healthy cross-bred sows (Landrace × Yorkshire) were randomly fed 4 groups diets as follows: basal (CTRL group), or containing only 600 mg/kg GOS (GOS + 100 HCA Low 200 High group) from d 85 weaning. Multiple parameters determined. Results There a trend shortening labor process ( p = 0.07) group Low/High group. Compared with CTRL group, increased average piglets weight at birth < 0.05), IgA concentration 0.05). In addition, serum triglyceride (TG) lower (T-AOC) higher 0.05) than farrowing. Serum catalase (CAT) activities The 16S rRNA analysis showed that combination high-dose shaped composition different stages (d 107 gestation, G107; 0 lactation, L0; 7 L7). At phylum level, relative abundance Bacteroidota Desulfobacterota G107, , Proteobacteria L0, Planctomycetota L7 Spearman correlation Streptococcus positively correlated TG but negatively Conclusion investigation demonstrated administration conjunction (HCA), nutrient restrictions further improved their milk quality. observed beneficial supplementation could potentially be linked an improvement disorders

Language: Английский

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Effect of Lactobacillus plantarum KSFY01 on the exercise capacity of D-galactose-induced oxidative stress-aged mice

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Dec. 22, 2022

Objectives Aging is a process that involves comprehensive physiological changes throughout the body, and improvements in exercise capacity of individuals may delay aging relieve fatigue. Probiotics are subject to ongoing research investigate their antioxidant properties. The purpose this study was effect probiotic Lactobacillus plantarum KSFY01 ( L. KSFY01) on tolerance mice induced into state accelerated by oxidative stress. Methods A mouse model established using D-galactose induce bacteria isolated from fermented yak yogurt. improvement aging-accelerated evaluated measuring running time until exhaustion, histopathological sections, related biochemical indicators, underlying gene expression. Results oral administration prolonged reduced creatine kinase (CK), alanine aminotransferase (ALT), aspartate aminotransferasem (AST) levels. From study, we observed significantly improved alleviated liver damage. Treatment with blood urea nitrogen (BUN), lactic acid (LD) accumulation, lactate dehydrogenase (LDH) elevations produced state, also reversed muscle glycogen (MG). Overall, could effectively improve metabolite thereby relieving fatigue exercised mice. results indices vivo showed intervention increased activity enzymes, decreased level malondialdehyde (MDA), restored balance between systems fatigued By investigating molecular mechanism, our decline expression levels nuclear factor-erythroid 2 factor (Nrf2) signaling pathway-related factors body’s capacity. We determined mechanism responsible for mainly activation Nrf2 pathway. dose-dependent, increasing dosage probiotic. Conclusion This demonstrated exerts effects athletic ability These findings significance development utilization resources.

Language: Английский

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Improvements of Age-Related Cognitive Decline in Mice by Lactobacillus helveticus WHH1889, a Novel Strain with Psychobiotic Properties

Nutrients, Journal Year: 2023, Volume and Issue: 15(17), P. 3852 - 3852

Published: Sept. 3, 2023

A gradual decline in cognitive function occurs with age. Accumulating evidence suggests that certain probiotic strains exert beneficial effects on age-related decline. Our previous study revealed Lactobacillus helveticus WHH1889 attenuated symptoms of anxiety and depression depressed mice via shaping the 5-hydroxytryptamine (5-HT) 5-hydroxytryptophan (5-HTP) metabolism gut microbial community, indicating psychobiotic potential WHH1889. In present study, were investigated. was orally administrated (1 × 109 CFU/day) for twelve weeks aged mice, their behaviors, neurochemical factors, cognitive-related gene expressions, neuroinflammation, serum tryptophan pathway-targeted metabolic profiling, as well microbiome composition assessed. demonstrated improvement behaviors novel object recognition test (NORT), active shuttle avoidance (ASAT), Y-maze test, passive (PAT). The hippocampal neuronal loss; declined concentrations BDNF, 5-HT, 5-HTP; decreased expressions neurodegeneration biomarkers; increased production inflammatory cytokines restored by addition, 5-HT/5HTP levels tryptophan-derived metabolites (e.g., kynurenine, xanthurenic acid, 3-hydroxykynurenine, 3-hydroxyanthranilic acid). Furthermore, to shape microbiota community reversing relative abundances Bacteroidota Firmicutes. findings suggest L. exerted improving which associated modulation 5-HT 5-HTP composition. supplementation may therefore be a promising therapeutic agent deficits.

Language: Английский

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