Journal of Economic Entomology,
Journal Year:
2023,
Volume and Issue:
116(3), P. 983 - 992
Published: April 29, 2023
Abstract
The
fall
armyworm
(FAW)
Spodoptera
frugiperda
was
first
found
in
China
2018.
In
other
countries,
FAW
has
evolved
corn
and
rice
strain
biotypes.
It
is
not
possible
to
identify
these
strains
based
on
morphology.
addition,
very
similar
appearance
several
common
pests.
These
situations
bring
great
challenges
the
population
management
of
FAW.
this
study,
we
developed
a
rapid
identification
method
PCR-RFLP
distinguish
two
from
lepidopteran
A
697
bp
mitochondrial
cytochrome
c
oxidase
I
(COI)
cloned
sequenced
FAW,
litura,
exigua,
Mythimna
separata.
COI
fragments
species
revealed
unique
digestion
patterns
created
by
three
enzymes
(Tail,
AlWN
I,
BstY
II).
Thus,
four
can
be
distinguished
each
other.
enzyme
Ban
recognized
SNP
site
638
triosephosphate
isomerase
(Tpi)
fragment
Tpi
cut
into
bands.
However,
could
digested.
Using
method,
all
28
samples
collected
different
host
plants
locations
were
identified
as
strain.
This
suggests
that
yet
invaded
China.
allows
discrimination
Lepidopteran
pests
distinguishes
strains.
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
72(33), P. 18365 - 18377
Published: Aug. 6, 2024
Host-symbiont
interaction
plays
a
crucial
role
in
determining
the
host's
fitness
under
toxic
stress,
as
observed
numerous
insect
species.
However,
mechanism
of
symbionts
involved
detoxification
insecticides
remains
poorly
known.
In
this
study,
through
microbiome,
proteomic,
and
genomic
analysis,
we
identified
prevalent
symbiont,
Journal of Agricultural and Food Chemistry,
Journal Year:
2023,
Volume and Issue:
71(2), P. 1091 - 1099
Published: Jan. 4, 2023
Fall
armyworm
(Spodoptera
frugiperda)
is
a
major
migratory
pest
around
the
entire
world
that
causes
severe
damage
to
agriculture.
We
designed
and
synthesized
series
of
novel
isoxazoline
derivatives
based
on
previously
discovered
active
compound
H13
find
new
effective
candidates
against
S.
frugiperda.
Most
them
showed
excellent
insecticidal
activity.
In
addition,
three-dimensional
quantitative
structure-activity
relationship
model
was
established,
F32
results.
The
bioassay
result
exhibited
activity
frugiperda
(LC50
=
3.46
mg/L),
which
substantially
better
than
positive
control
fipronil
78.8
mg/L).
Furthermore,
an
insect
γ-aminobutyric
acid
(GABA)
enzyme-linked
immunosorbent
assay
indicated
can
upregulate
content
GABA
in
insects
manner
similar
fipronil.
Molecular
docking
hydrophobic
effect
hydrogen-bond
interactions
are
vital
factors
between
binding
receptors.
All
these
results
suggest
could
be
employed
as
lead
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
72(13), P. 6889 - 6899
Published: March 21, 2024
Spodoptera
frugiperda
is
primarily
controlled
through
chemical
insecticides.
Our
RNA-seq
data
highlight
the
overexpression
of
GSTs4
in
indoxacarb-resistant
S.
frugiperda.
However,
exact
role
indoxacarb
resistance
and
its
regulatory
mechanisms
remains
elusive.
Therefore,
we
investigated
functional
explored
underlying
post-transcriptional
mechanisms.
was
highly
overexpressed
(27.6-fold)
strain,
silencing
significantly
increases
susceptibility
to
indoxacarb,
increasing
mortality
by
27.3%.
miR-317-3p
miR-283-5p
can
bind
3′UTR
GSTs4,
targeting
relationship
confirmed
dual-luciferase
reporter
assays.
Injecting
agomirs
reduces
levels
64.8
42.3%,
respectively,
resulting
an
increased
indoxacarb.
Conversely,
administration
miR-283-5pantagomirs
expression
larval
These
findings
demonstrate
that
contribute
regulating
GSTs4.
Journal of Agricultural and Food Chemistry,
Journal Year:
2023,
Volume and Issue:
71(18), P. 6859 - 6870
Published: May 1, 2023
With
the
continuous
evolution
of
insect
resistance,
it
is
a
tremendous
challenge
to
control
fall
armyworm
(Spodoptera
frugiperda)
with
traditional
insecticides.
To
solve
this
pending
issue,
series
novel
isoxazoline
derivatives
containing
diaryl
ether
structures
were
designed
and
synthesized,
most
target
compounds
exhibited
excellent
insecticidal
activity.
Based
on
three-dimensional
quantitative
structure–activity
relationship
(3D-QSAR)
model
analysis,
we
further
optimized
molecular
structure
compound
L35
obtained
tested
for
its
Compound
(LC50
=
1.69
mg/L)
activity
against
S.
frugiperda,
which
was
better
than
those
commercial
fipronil
70.78
indoxacarb
5.37
mg/L).
The
enzyme-linked
immunosorbent
assay
showed
that
could
upregulate
levels
GABA
in
insects.
In
addition,
docking
transcriptomic
results
also
indicated
may
affect
nervous
system
frugiperda
by
acting
receptors.
Notably,
through
high-performance
liquid
chromatography
(HPLC),
able
obtain
two
enantiomers
L35,
test
revealed
S-(+)-L35
44
times
more
active
R-(−)-L35
frugiperda.
This
study
established
chemistry
basis
mechanistic
foundations
future
development
pesticide
candidates
armyworms.
Journal of Insect Science,
Journal Year:
2025,
Volume and Issue:
25(1)
Published: Jan. 1, 2025
The
fall
armyworm,
Spodoptera
frugiperda
J.E.
Smith
(Lepidoptera:
Noctuidae),
is
a
well-known
agricultural
pest
in
North
and
South
America
has
invaded
Africa,
the
Far
East,
Australia
past
decade.
To
investigate
integrated
management
of
S.
frugiperda,
sublethal
impacts
emamectin
benzoate
were
studied.
Leaf-dipping
bioassays
used
to
effects
(LC10
LC30)
concentrations
on
demographic
parameters,
data
interpreted
based
age-stage,
two-sex
life
table
theory.
results
showed
that
developmental
time
larvae
was
prolonged
while
fecundity
decreased
after
concentration
treatment.
Furthermore,
intrinsic
finite
rates
increase,
as
well
net
reproductive
rate,
significantly
declined
following
LC30
exposure,
whereas
average
generation
extended
comparison
control
group.
rate
increase
(rm)
dropped
0.14
(LC10)
0.13
(LC30)/day,
compared
group
(0.18/day).
(R0)
from
775.40
303.10
193.30
LC10
treatment,
respectively.
In
this
study,
adversely
affected
time,
fecundity,
parameters
frugiperda.
Insect Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 16, 2025
Abstract
Spodoptera
frugiperda
(Lepidoptera:
Noctuidae)
is
a
serious
invasive
pest,
which
has
attracted
concern
regarding
the
effectiveness
of
environmental
bioinsecticide
as
substitution
for
synthetic
insecticide
in
controlling
its
damage
to
numerous
agricultural
crops
recent
years.
Hence,
laboratory
and
field
experiments
have
investigated
insecticidal
toxicity
S.
by
5
insecticides
determined
activity
acetylcholinesterase
(AChE)
detoxifying
enzymes,
namely
mixed
function
oxidase
(MFO),
carboxylesterase
(CarE),
glutathione
S‐transferase
(GST)
on
3rd
instar
larvae
at
25%
lethal
concentration
(LC
25
)
explore
detoxification
mechanisms.
The
results
showed
that
most
effective
were
3.0%
emamectin
benzoate
(0.024
mg/L),
60
g/L
spinetoram
(0.282
1.3%
matrine
(0.380
mg/L)
48
h
depending
LC
50
values.
efficiencies
over
80%
24
90%
72
h,
indicating
both
had
acute
long‐lasting
;
extended‐release
with
efficiency
h.
Emamectin
activated
activities
AChE,
MFO
GST,
decreased
CarE
activity;
cyantraniliprole
induced
an
increase
4
enzymes;
spinosad
increased
MFO,
but
not
GST.
Furthermore,
inhibiting
effect
AChE
acceleration
Overall
obviously
depicted
semi‐synthetic
recommended
control
toxicity.
Moreover,
this
study
will
provide
basic
information
sustainable
under
conditions
China.
