MiR-146b-5p enriched bioinspired exosomes derived from fucoidan-directed induction mesenchymal stem cells protect chondrocytes in osteoarthritis by targeting TRAF6 DOI Creative Commons
Chao Lou,

Hongyi Jiang,

Zhongnan Lin

et al.

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Dec. 18, 2023

Abstract Osteoarthritis (OA) is a common degenerative joint disease characterized by progressive cartilage degradation and inflammation. In recent years, mesenchymal stem cells (MSCs) derived exosomes (MSCs-Exo) have attracted widespread attention for their potential role in modulating OA pathology. However, the unpredictable therapeutic effects of been significant barrier to extensive clinical application. this study, we investigated whether fucoidan-pretreated MSC-derived (F-MSCs-Exo) could better protect chondrocytes osteoarthritic joints elucidate its underlying mechanisms. order evaluate F-MSCs-Exo osteoarthritis, both vitro vivo studies were conducted. MiRNA sequencing was employed analyze MSCs-Exo F-MSCs-Exo, enabling identification differentially expressed genes exploration mechanisms behind protective osteoarthritis. Compared MSCs-Exo, demonstrated superior effectiveness inhibiting inflammatory responses extracellular matrix rat chondrocytes. Moreover, exhibited enhanced activation autophagy revealed that miR-146b-5p emerged as promising candidate mediator chondroprotective function with TRAF6 identified downstream target. conclusion, our research results demonstrate encapsulated effectively inhibits activation, thereby suppressing degradation, while promoting chondrocyte protection cells. Consequently, development approach combining fucoidan provides strategy treatment

Language: Английский

Phenolics from Dendrobium officinale Leaf Ameliorate Dextran Sulfate Sodium-Induced Chronic Colitis by Regulating Gut Microbiota and Intestinal Barrier DOI
Peiyi Wang, Ming Cai, Kai Yang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(44), P. 16630 - 16646

Published: Oct. 26, 2023

The increasing incidence of colitis and the side effects its therapeutic drugs have led to search for compounds natural origin, including phenolics, as new treatments colitis. In this study, potential mechanism Dendrobium officinale leaf phenolics (DOP) on relief dextran sulfate sodium (DSS)-induced was explored. results showed that DOP treatment 36 days reduced symptoms caused by DSS, reduction disease activity index alleviation colonic tissue damage. addition, downregulated expression key proteins TLR4/NF-κB signaling pathway production inflammatory cytokines. Furthermore, could enhance tight junction ZO-1, Occludin, Claudin-1 restore intestinal mucosal barrier function. also effectively regulates disordered flora enhances short-chain fatty acids, which is beneficial in modulating gut internal environmental homeostasis, inhibiting inflammation, restoring barrier. These findings indicated can ameliorate DSS-induced chronic regulating microbiota, barrier, it a promising ingredient from D. officinale.

Language: Английский

Citations

43
A High-Fat Diet Increases the Characteristics of Gut Microbial Composition and the Intestinal Damage Associated with Non-Alcoholic Fatty Liver Disease DOI Open Access

Xiaoyang Zhu,

Jiajia Cai, Yifu Wang

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(23), P. 16733 - 16733

Published: Nov. 24, 2023

The prevalence of non-alcoholic fatty liver disease (NAFLD) is increasing annually, and emerging evidence suggests that the gut microbiota plays a causative role in development NAFLD. However, NAFLD remains unclear warrants further investigation. Thus, C57BL/6J mice were fed high-fat diet (HFD), we found HFD significantly induced obesity increased accumulation intrahepatic lipids, along with alterations serum biochemical parameters. Moreover, it was observed also impaired barrier integrity. It revealed via 16S rRNA gene sequencing microbial diversity, which enriched Colidextribacter, Lachnospiraceae-NK4A136-group, Acetatifactor, Erysipelatoclostridium. Meanwhile, reduced abundance Faecalibaculum, Muribaculaceae, Coriobacteriaceae-UCG-002. predicted metabolic pathways suggest enhances chemotaxis functional activity associated flagellar assembly, while risk intestinal pathogen colonization inflammation. And phosphotransferase system, streptomycin biosynthesis, starch/sucrose metabolism exhibited decreases. These findings reveal composition predictive functions microbiome NAFLD, corroborating association between providing novel insights into its potential application research for patients.

Language: Английский

Citations

35
Recent progress in plant-derived polysaccharides with prebiotic potential for intestinal health by targeting gut microbiota: a review DOI
Xiaozhen Wang, Xia Li, Luyao Zhang

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2023, Volume and Issue: 64(33), P. 12242 - 12271

Published: Aug. 31, 2023

Natural products of plant origin are high interest and widely used, especially in the food industry, due to their low toxicity wide range bioactive properties. Compared other components, safety polysaccharides has been generally recognized. As dietary fibers, plant-derived mostly degraded intestine by polysaccharide-degrading enzymes secreted gut microbiota, have potential prebiotic activity both non-disease disease states, which should not be overlooked, terms involvement treatment intestinal diseases promotion health. This review elucidates regulatory effects on microbiota summarizes mechanisms involved targeting for diseases. Further, structure-activity relationships between different structural types occurrence further explored. Finally, practical applications production packaging summarized discussed, providing important references expanding application industry or developing functional supplements.

Language: Английский

Citations

34
Effect of the β-glucan from Lentinus edodes on colitis-associated colorectal cancer and gut microbiota DOI

Ningyue Liu,

Siwei Zou,

Conghua Xie

et al.

Carbohydrate Polymers, Journal Year: 2023, Volume and Issue: 316, P. 121069 - 121069

Published: May 26, 2023

Language: Английский

Citations

33
Bergamot polysaccharides relieve DSS-induced ulcerative colitis via regulating the gut microbiota and metabolites DOI
Yiren Yang, Yuwei Zhang, Jiangping Song

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 253, P. 127335 - 127335

Published: Oct. 18, 2023

Language: Английский

Citations

33
Urolithin A Alleviates Colitis in Mice by Improving Gut Microbiota Dysbiosis, Modulating Microbial Tryptophan Metabolism, and Triggering AhR Activation DOI

Mingxia Ma,

Yanxin Wang, Siqing Fan

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(20), P. 7710 - 7722

Published: May 11, 2023

Urolithin A (UroA) is a microbial metabolite derived from ellagitannins and ellagic acid with good bioavailability. In this study, we explored the anticolitis activity of UroA clarified mechanism by 16S rDNA sequencing metabonomics. alleviated dextran sulfate sodium (DSS)-induced colitis in mice, characterized decreased disease index, increased colon length, improved colonic histopathological lesions, along inhibited phosphorylation mitogen-activated protein kinase signaling pathway. addition, gut microbiota dysbiosis modulated metabolome. Furthermore, targeted metabolomics focused on tryptophan catabolites showed that significantly production indole-3-aldehyde (IAld) subsequently led to expression aryl hydrocarbon receptor (AhR) promoted serum content IL-22 mice colitis. Collectively, our data identified novel improving dysbiosis, modulating metabolism, promoting IAld production, triggering AhR/IL-22 axis activation. However, limitation noted study these beneficial effects were found at 50 μM vitro 20 mg/kg vivo, which nonphysiological concentrations.

Language: Английский

Citations

24
Fucoidan enhances the anti-tumor effect of anti-PD-1 immunotherapy by regulating gut microbiota DOI
H.-F. Li,

Tieying Dong,

Meng Tao

et al.

Food & Function, Journal Year: 2024, Volume and Issue: 15(7), P. 3463 - 3478

Published: Jan. 1, 2024

Fucoidan potentiated the antitumour effect with anti-PD-1 monoclonal antibody via enhancing CD8 T cells function, increasing production of IFN-γ, TNF-α, and GZMB reducing suppression Treg in circulating system, which might be addressed by reshaping gut microbiota.

Language: Английский

Citations

15
Chemical Composition and Antibacterial and Antiulcerative Colitis Activities of Essential Oil from Pruni Semen DOI

Yali Wang,

Deng-Qin Lang,

Chao Wu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(2), P. 1096 - 1113

Published: Jan. 3, 2024

This study was sought to investigate the chemical composition and antibacterial antiulcerative colitis (UC) effects of essential oil from Pruni Semen (PSEO). A GC-MS assay showed that major compounds in PSEO were products amygdalin hydrolysis, which possessed great anti-inflammatory potential. In vitro experiments demonstrated treatment inhibited activity four kinds intestinal pathogens probably by disrupting cell wall. Further vivo studies administration significantly improved physiological indexes, attenuated histopathological characteristics, proinflammatory cytokine production dextran sulfate sodium (DSS)-induced UC mice. Network pharmacology molecular docking results predicted might prevent via regulating PI3K/AKT pathway. Western blotting immunofluorescence assays further conducted for verification, evidenced intervention regulated pathway expression its downstream proteins DSS-induced provide a new dietary strategy treatment.

Language: Английский

Citations

9
Fucoidan Improves Early Stage Diabetic Nephropathy via the Gut Microbiota–Mitochondria Axis in High-Fat Diet-Induced Diabetic Mice DOI

Zhaoyi Zhong,

Yangting Zhang,

Wei Yuan

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(17), P. 9755 - 9767

Published: April 18, 2024

Diabetic nephropathy (DN) is a common microvascular complication of diabetes. Fucoidan, polysaccharide containing fucose and sulfate group, ameliorates DN. However, the underlying mechanism has not been fully understood. This study aimed to explore effects fucoidan on DN in high-fat diet-induced diabetic mice. A total 90 C57BL/6J mice were randomly assigned six groups (n = 15) as follows: normal control (NC), diabetes mellitus (DM), metformin (MTF), low-dose (LFC), medium-dose (MFC), high-dose (HFC). technique based fluorescein isothiocyanate (FITC-sinistin) elimination kinetics measured percutaneously was applied determine glomerular filtration rate (GFR). After 24 weeks, sacrificed an early stage model confirmed by GFR hyperfiltration, elevated urinary creatinine, albumin, tubulointerstitial fibrosis, hypertrophy. Fucoidan significantly improved hyperfiltration renal fibrosis. An enriched SCFAs-producing bacteria increased acetic concentration cecum contents found groups, well ATP levels mitochondrial dysfunction. The inflammation fibrosis ameliorated through inhibiting MAPKs pathway. In conclusion, targeting microbiota–mitochondria axis ameliorating oxidative stress

Language: Английский

Citations

9
Huaier Polysaccharide Alleviates Dextran Sulphate Sodium Salt-Induced Colitis by Inhibiting Inflammation and Oxidative Stress, Maintaining the Intestinal Barrier, and Modulating Gut Microbiota DOI Open Access
Yifei Tang,

Wen‐Yin Xie,

Hongyu Wu

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(9), P. 1368 - 1368

Published: April 30, 2024

The incidence of ulcerative colitis (UC) is increasing annually, and UC has a serious impact on patients’ lives. Polysaccharides have gained attention as potential drug candidates for treating in recent years. Huaier (Trametes robiniophila Murr) fungus that been used clinically more than 1000 years, its bioactive polysaccharide components reported to possess immunomodulatory effects, antitumour potential, renoprotective effects. In this study, we aimed examine the protective effects mechanisms (HP) against UC. Based H2O2-induced oxidative stress model HT-29 cells dextran sulphate sodium salt (DSS)-induced model, demonstrated polysaccharides significantly alleviated DSS-induced (weight loss, elevated disease activity index (DAI) scores, colonic shortening). addition, HP inhibited inflammation intestinal barrier damage. It also promoted expression mucin Muc2. Furthermore, reduced abundance harmful bacteria Escherichia-Shigella beneficial Muribaculaceae_unclassified, Anaerotruncus, Ruminococcaceae_unclassified regulate flora disturbance caused by DSS. Nontargeted metabolomics revealed intervention would modulate metabolism promoting levels 3-hydroxybutyric acid, phosphatidylcholine (PC), phosphatidylethanolamine (PE). These results had ability mitigate suppressing inflammation, maintaining barrier, modulating flora. findings will expand our knowledge how functions offer theoretical foundation using prebiotic prevent

Language: Английский

Citations

9