Journal of Agricultural and Food Chemistry,
Journal Year:
2023,
Volume and Issue:
71(40), P. 14517 - 14526
Published: Sept. 29, 2023
Cytochrome
P450
plays
vital
roles
in
detoxifying
xenobiotics.
In
this
study,
SlCYP340A
and
SlCYP340L
expression
the
Spodoptera
litura
fat
body
SlCYP332A1,
SlCYP6AB12,
SlCYP6AB58,
SlCYP6AB59,
SlCYP6AN4
Malpighian
tubules
were
significantly
upregulated
after
cyantraniliprole
exposure,
SlCYP6AB58
SlCYP6AB59
levels
simultaneously
increased
gossypol
treatment.
Drosophila
ectopically
expressing
candidate
genes
showed
that
SlCYP6AN4,
conferred
tolerance.
The
overexpression
of
number
eggs
laid
under
Moreover,
knockdown
S.
mortality
Homology
modeling
molecular
docking
results
suggested
has
potential
to
bind
with
cyantraniliprole.
These
indicate
CYP3
CYP4
participate
detoxification
may
be
involved
tolerance
litura.
Bulletin of Entomological Research,
Journal Year:
2024,
Volume and Issue:
114(4), P. 482 - 490
Published: May 6, 2024
Abstract
Carboxylesterases
(CarEs)
is
an
important
detoxification
enzyme
system
in
phase
Ⅰ
participating
insecticides
resistance.
In
our
previous
study,
SlCarE054,
a
CarEs
gene
from
lepidoptera
class,
was
screened
out
to
be
upregulated
pyrethroids
and
organophosphates
resistant
population.
Its
overexpression
verified
two
field-collected
populations
of
Spodoptera
litura
(Lepidoptera:
Noctuidae)
by
qRT-PCR.
Spatiotemporal
expression
results
showed
that
SlCarE054
highly
expressed
the
pupae
stage
digestive
tissue
midgut.
To
further
explore
its
role
resistance,
metabolism
activity
determined
UPLC.
recombinant
protein
significant
cyhalothrin
fenvalerate,
but
not
phoxim
or
chlorpyrifos.
The
metabolic
β
-cypermethrin
stereoselectivity,
with
higher
θ
than
enantiomer
α
-cypermethrin.
metabolite
identified
as
3-phenoxybenzaldehyde.
Further
modelling
docking
analysis
indicated
-cypermethrin,
fenvalerate
could
bind
catalytic
triad
3D
structure
SlCarE054.
interaction
also
lowest
binding
energy.
Our
work
provides
evidence
play
roles
resistance
S.
.
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
72(19), P. 10958 - 10969
Published: May 4, 2024
Demand
for
the
exploration
of
botanical
pesticides
continues
to
increase
due
detrimental
effects
synthetic
chemicals
on
human
health
and
environment
development
resistance
by
pests.
Under
guidance
a
bioactivity-guided
approach
HSQC-based
DeepSAT,
16
coumarin
derivatives
were
discovered
from
leaves
