Peptide Science,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 15, 2024
ABSTRACT
Bioactive
peptides
(BAPs)
have
attracted
considerable
interest
in
scientific
research
due
to
their
heterogeneity
sequence
and
structure,
which
underpins
various
biological
functionalities.
In
this
context,
goat
casein,
an
abundant
by‐product
of
the
dairy
industry,
emerges
as
a
valuable
source
BAPs.
The
present
study
undertook
meticulous
evaluation
bioactive
potentials
casein‐derived
through
integrated
approach
combining
computational
simulations,
high‐throughput
screening,
molecular
docking
techniques.
initial
phase
involved
enzymatic
digestion
milk
casein
using
trypsin,
followed
by
identification
via
liquid
chromatography–tandem
mass
spectrometry
(LC–MS/MS),
uncovering
total
597
peptides.
Subsequent
prioritization
PeptideRanker
algorithm
identified
70
exhibiting
potential
bioactivity,
denoted
scores
above
0.8.
Advanced
screening
employing
BIOPEP
database
AutoDock
CAMPR4
tools
facilitated
elucidation
16
antioxidant,
59
hypotensive,
63
hypoglycemic,
hypolipidemic,
25
antimicrobial
Molecular
studies
further
elucidated
spontaneous
nature
interactions
between
respective
receptors,
predominantly
mediated
hydrogen
bonding
hydrophobic
interactions.
Four
specifying
all
activities
simultaneously
were
synthesized,
verified
vitro
experiments.
These
results
not
only
highlight
effective
strategy
for
but
also
underscore
utilizing
viable
functional
food
ingredients.
This
thereby
contributes
significantly
expanding
field
research,
suggesting
sustainable
explore
by‐products.
Life,
Journal Year:
2025,
Volume and Issue:
15(2), P. 225 - 225
Published: Feb. 4, 2025
Hempseeds,
from
the
Cannabis
sativa
plant,
and
its
derivates
are
a
versatile
food
option
for
various
dietary
preferences.
Due
to
their
aminoacidic
profile,
researchers
have
studied
presence
of
bioactive
peptides
in
hempseed
proteins.
In
this
study,
water-soluble
fraction
protein
was
extracted,
derived
were
analyzed.
The
investigation
focused
on
biological
function,
particularly
antioxidant
activity.
Several
functions
arisen,
such
as
angiotensin-converting
enzyme
inhibition
activity,
dipeptidyl-peptidase
IV,
III
inhibition,
ubiquitin-mediated
proteolysis
activation.
hydrolysates
show
greater
2,2-azinobis-[3-ethylbenzothiazoline-6-sulphonic
acid
(ABTS)
radical
scavenging
activity
compared
proteins
(97.95
±
4.48
versus
81.04
10.63).
Furthermore,
impact
these
U937
cell
line
evaluated
assess
viability
potential
role
modulating
inflammation
associated
with
gastrointestinal
autoimmune
diseases.
Protein
treatment
resulted
significant
reduction
viability,
opposed
hydrolysates,
which
did
not
affect
it.
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(5), P. 2222 - 2222
Published: Feb. 28, 2025
There
is
a
growing
demand
for
natural
and
potent
α-glucosidase
inhibitors
due
to
the
rising
prevalence
of
diabetes.
In
this
study,
newly
identified
inhibitory
peptides
were
from
tryptic
hydrolysate
hemp
seed
proteins
based
on
peptidomics
in
silico
analysis.
A
total
424
peptides,
primarily
derived
four
cupin-type-1
domain-containing
proteins,
identified,
13
ultimately
selected
validation
their
higher
PeptideRanker
scores,
solubility,
non-toxicity,
favorable
ADMET
properties.
Molecular
docking
revealed
that
these
interacted
with
via
hydrogen
bonding
hydrophobic
interactions.
Among
them,
three
novel
peptides-NPVSLPGR
(-8.7
kcal/mol),
LSAERGFLY
(-8.5
PDDVLANAF
(-8.4
kcal/mol)-demonstrated
activity
lower
binding
energies
than
acarbose
(-8.1
first
approved
inhibitor
type
2
diabetes
treatment.
The
molecular
mechanism
analysis
NPVSLPGR
inhibited
by
simultaneously
blocking
substrate
entry
through
occupying
entrance
active
site
gorge
preventing
catalysis
sites.
contrast,
peptide
exerted
effects
gorge.
dynamics
simulation
validated
stability
complexes
provided
additional
insights
into
determined
docking.
These
findings
contribute
essential
knowledge
advancement
offer
promising
approach
effectively
manage
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
72(11), P. 5746 - 5756
Published: March 7, 2024
Alcohol
dehydrogenase
(ADH)
is
a
crucial
rate-limiting
enzyme
in
alcohol
metabolism.
Our
previous
research
found
that
ethanol-induced
intracellular
extracts
of
Lactococcus
lactis
(L.
lactis)
could
enhance
metabolism
mice,
but
the
responsible
compounds
remain
unidentified.
The
study
aimed
to
screen
potential
ADH-activating
peptides
from
L.
using
virtual
screening
and
molecular
docking
calculation.
Among
them,
pentapeptide
FAPEG
might
bind
ADH
through
hydrophobic
interaction
hydrogen
bonds,
then
enhancing
activity.
Spectroscopy
analysis
further
investigated
peptide-enzyme
between
ADH,
including
changes
amino
acid
residue
microenvironment
secondary
structural
alterations.
Furthermore,
protect
against
alcoholic
liver
injury
(ALI)
mice
by
reducing
blood
concentration,
activity
antioxidant
enzymes,
attenuating
alcohol-induced
hepatotoxicity,
which
was
related
activation
Nrf2/keap1/HO-1
signaling
pathway.
provided
preliminary
evidence
generation
has
preventing
ALI
silico
prediction
vivo
validation
approaches.
Foods,
Journal Year:
2025,
Volume and Issue:
14(7), P. 1149 - 1149
Published: March 26, 2025
Hemp
is
a
multiuse
crop
used
for
fiber,
food,
and
medicinal
purposes.
The
seed
of
hemp
has
attracted
great
attention
as
good
plant
protein
resource
with
remarkable
nutritional
biological
properties.
However,
the
application
(HSP)
limited
due
to
its
unsatisfactory
functional
Physical,
chemical,
technologies
have
been
explored
modify
structure
HSP
improve
functionality.
investigation
activity
derived
peptide
deal
intestinal,
metabolic,
muscle
concerns
broadened
utilization
in
healthy
products.
Therefore,
current
review
performed
summarize
recent
research
progress
on
novel
extraction
modification
HSP,
well
purification
identification
active
peptide.
multi-functional
multi-bioactive
properties
adverse
effects
are
also
depicted
facilitate
their
potential
applications
food
industry.
