Integrative Analysis of Flavonoid Pathways in Rice: Enhancing Heat Tolerance DOI Creative Commons
Xiaojie Wu,

Lingfang Yang,

Jun Han

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 27, 2024

Abstract Background Plants tend to produce special metabolites resist biotic or abiotic invasions, in which flavonoid-mediated defense responses play an important role. Result In our previous work, the rel1-D mutant was obtained by T-DNA insertion. Nearly all ZH11 died after 42 ℃ treatment, while nearly half of mutants survived. By transcriptomic and metabolomic analysis leaves, 1184 differentially expressed genes (DEGs) 126 accumulated (DAMs) were identified, most these DEGs DAMs enriched biosynthesis-related pathways such as L-Phenylalanine pathway, flavonoid biosynthesis pathway phenol pathway. Furthermore, a correlation network involved phenotypic traits constructed based on metabolites. Conclusion Potential regulated REL1 identified. may affect accumulation regulating expression key influence heat tolerance rice.

Language: Английский

Structural and Interactional Analysis of the Flavonoid Pathway Proteins: Chalcone Synthase, Chalcone Isomerase and Chalcone Isomerase-like Protein DOI Open Access
Jacob A. Lewis, Eric P. Jacobo, Nathan A. Palmer

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 5651 - 5651

Published: May 22, 2024

Chalcone synthase (CHS) and chalcone isomerase (CHI) catalyze the first two committed steps of flavonoid pathway that plays a pivotal role in growth reproduction land plants, including UV protection, pigmentation, symbiotic nitrogen fixation, pathogen resistance. Based on obtained X-ray crystal structures CHS, CHI, isomerase-like protein (CHIL) from same monocotyledon,

Language: Английский

Citations

6
Harnessing Aromatic Properties for Sustainable Bio-valorization of Lignin Derivatives into Flavonoids DOI Creative Commons
Siyu Zhu, Na Li, Zhihua Liu

et al.

Green Carbon, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Metabolic Engineering of Escherichia coli BL21(DE3) for 2′-Fucosyllactose Synthesis in a Higher Productivity DOI
Na Li, Yan Song,

Hongzhi Xia

et al.

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

2′-Fucosyllactose (2′-FL) is the most abundant human milk oligosaccharides (HMOs). 2′-FL exhibits great benefits for infant health, such as preventing infantile diarrhea and promoting growth of intestinal probiotics. The microbial cell factory technique has shown promise massive production 2′-FL. Here, we aimed to construct a recombinant E. coli BL21(DE3) strain hyperproduction Initially, multicopy genomic integration expression lactose permease gene lacY reduced formation byproducts. Furthermore, more efficient Shine–Dalgarno sequence was used replace wild-type in manC-manB gmd-wcaG clusters, which significantly increased titer. Based on these results, overexpressed sugar efflux transporter SetA knocked out pgi gene. This further improved synthesis when glycerol sole carbon source. Finally, new α-1,2-fucosyltransferase identified Neisseria sp., exhibited higher capacity production. Fed-batch fermentation produced 141.27 g/L 45 h with productivity 3.14 × h. rate achieved highest recorded levels, indicating potential engineered BL21 (DE3) strains use industrial

Language: Английский

Citations

0
Identification of two new flavone 4'-O-methyltransferases and their application in de novo biosynthesis of (2S)-hesperetin in Yarrowia lipolytica DOI Creative Commons
Yiyun Wang, Rong Huang, Song Gao

et al.

Synthetic and Systems Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Engineering a Plant Polyketide Synthase for the Biosynthesis of Methylated Flavonoids DOI Creative Commons
Bo Peng, Lili Zhang, Siqi He

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 72(1), P. 529 - 539

Published: Dec. 18, 2023

Homoeriodictyol and hesperetin are naturally occurring O-methylated flavonoids with many health-promoting properties. They produced in plants low abundance as complex mixtures of similar compounds that difficult to separate. Synthetic biology offers the opportunity produce various a targeted, bottom-up approach engineered microbes high product titers. However, production is currently still highly inefficient. In this study, we investigated combination enzymes had previously been shown support homoeriodictyol

Language: Английский

Citations

9
Novel Catechol O-methyltransferases from Lentinula edodes Catalyze the Generation of Taste-Active Flavonoids DOI
Jean-Philippe Kanter, Lars Milke,

Judith K. Metz

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(19), P. 11002 - 11012

Published: May 3, 2024

Due to the increasing demand for natural food ingredients, including taste-active compounds, enzyme-catalyzed conversions of substrates, such as flavonoids, are promising tools align with principles Green Chemistry. In this study, a novel

Language: Английский

Citations

3
Microbial production of the plant flavanone hesperetin from caffeic acid DOI Creative Commons
Erik K. R. Hanko, João Correia, Caio S. Souza

et al.

BMC Research Notes, Journal Year: 2023, Volume and Issue: 16(1)

Published: Nov. 18, 2023

Abstract Objective Hesperetin is an important O -methylated flavonoid produced by citrus fruits and of potential pharmaceutical relevance. The microbial biosynthesis hesperetin could be a viable alternative to plant extraction, as extracts often yield complex mixtures different flavonoids making it challenging isolate pure compounds. In this study, was from caffeic acid in the host Escherichia coli . We combined previously optimised pathway for intermediate flavanone eriodictyol with combinatorial library plasmids expressing three candidate -methyltransferases. Moreover, we endeavoured improve position specificity CCoAOMT7, -methyltransferase Arabidopsis thaliana that has been demonstrated -methylate both para - meta -position, thus leading mixture homoeriodictyol. Results best performing our screen found which produce up 14.6 mg/L 3.8 homoeriodictyol 3 mM E. 5-alpha. Using platform enzyme engineering scans mutational space selected key positions, predicting their structures using homology modelling inferring catalytic improvement docking simulations, were able identify CCoAOMT7 mutant two-fold higher hesperetin. mutant’s activity, however, considerably diminished. Our findings suggest can created central carbon metabolism following introduction pathway.

Language: Английский

Citations

5
Heterologous Naringenin Production in the Filamentous Fungus Penicillium rubens DOI Creative Commons
Bo Peng, Lin Dai, Riccardo Iacovelli

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(51), P. 20782 - 20792

Published: Dec. 16, 2023

Naringenin is a natural product with several reported bioactivities and the key intermediate for entire class of plant flavonoids. The translation flavonoids into modern medicine as pure compounds often hampered by their low abundance in nature difficult chemical synthesis. Here, we investigated possibility to use filamentous fungus Penicillium rubens host flavonoid production. P. well-characterized, highly engineered, traditional "workhorse" production β-lactam antibiotics. We integrated two genes encoding enzymes naringenin biosynthesis pathway genome secondary metabolite-deficient 4xKO strain. After optimization fermentation conditions, obtained an excellent molar yield from fed p-coumaric acid (88%) titer 0.88 mM. Along accumulation over 36 h, however, also observed rapid degradation naringenin. Based on high-resolution mass spectrometry analysis, propose 4xKO, which distinct other flavonoid-converting pathways fungi. Our work demonstrates that promising recombinant production, it represents interesting starting point further investigation utilization biomass

Language: Английский

Citations

5
Integrative Analysis of Flavonoid Pathways in Rice: Enhancing Heat Tolerance DOI Creative Commons
Xiaojie Wu,

Lingfang Yang,

Jun Han

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 27, 2024

Abstract Background Plants tend to produce special metabolites resist biotic or abiotic invasions, in which flavonoid-mediated defense responses play an important role. Result In our previous work, the rel1-D mutant was obtained by T-DNA insertion. Nearly all ZH11 died after 42 ℃ treatment, while nearly half of mutants survived. By transcriptomic and metabolomic analysis leaves, 1184 differentially expressed genes (DEGs) 126 accumulated (DAMs) were identified, most these DEGs DAMs enriched biosynthesis-related pathways such as L-Phenylalanine pathway, flavonoid biosynthesis pathway phenol pathway. Furthermore, a correlation network involved phenotypic traits constructed based on metabolites. Conclusion Potential regulated REL1 identified. may affect accumulation regulating expression key influence heat tolerance rice.

Language: Английский

Citations

0