Environmental science and engineering, Journal Year: 2024, Volume and Issue: unknown, P. 163 - 188
Published: Dec. 31, 2024
Language: Английский
International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140068 - 140068
Published: Jan. 1, 2025
Language: Английский
Citations
0
Synthetic biology and engineering, Journal Year: 2025, Volume and Issue: 3(1), P. 10001 - 10001
Published: Jan. 1, 2025
Language: Английский
Citations
0
iScience, Journal Year: 2025, Volume and Issue: 28(4), P. 112202 - 112202
Published: March 11, 2025
Highly flexible regions were targeted for successful modification to enhance enzyme stability. However, this approach could not cover all key sites. Residues in certain rigid are also crucial protein This study proposed a short-loop engineering strategy that explores "sensitive residues" and mutated them hydrophobic residues with large side chains fill the cavities, thereby improving thermal identified sensitive of three enzymes: lactate dehydrogenase from Pediococcus pentosaceus, urate oxidase Aspergillus flavus, D-lactate Klebsiella pneumoniae. Under guidance strategy, half-life periods these enzymes 9.5, 3.11, 1.43 times higher than wild type, respectively. We standard procedure developed visualization plugin, offering new insights into stability modification.
Language: Английский
Citations
0
Green Synthesis and Catalysis, Journal Year: 2025, Volume and Issue: unknown
Published: March 1, 2025
Language: Английский
Citations
0
MedComm, Journal Year: 2024, Volume and Issue: 5(11)
Published: Nov. 1, 2024
Abstract Identification of therapeutic targets can directly elucidate the mechanism and effect drug therapy, which is a central step in development. The disconnect between protein phenotypes under complex mechanisms hampers comprehensive target understanding. Metabolomics, as systems biology tool that captures phenotypic changes induced by exogenous compounds, has emerged valuable approach for identification. A overview was provided this review to illustrate principles advantages metabolomics, delving into application metabolomics This outlines various metabolomics‐based methods, such dose–response stable isotope‐resolved multiomics, identify key enzymes metabolic pathways affected substances through dose‐dependent metabolite–drug interactions. Emerging techniques, including single‐cell artificial intelligence, mass spectrometry imaging, are also explored their potential enhance discovery. emphasizes metabolomics' critical role advancing our understanding disease accelerating targeted development, while acknowledging current challenges field.
Language: Английский
Citations
2
Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(42), P. 23082 - 23096
Published: Oct. 10, 2024
Bacterial chitinases are integral in breaking down chitin, the natural polymer crustacean and insect exoskeletons. Their increasing utilization across various sectors such as agriculture, waste management, biotechnology, food processing, pharmaceutical industries highlights their significance biocatalysts. The current review investigates scientific strategies to maximize efficiency production of bacterial for industrial use. Our goal is optimize heterologous process using physical, molecular, computational tools. Physical methods focus on isolating, purifying, characterizing from sources ensure optimal conditions maximum enzyme activity. Molecular techniques involve gene cloning, site-directed mutation, CRISPR-Cas9 editing an approach creating with improved catalytic activity, substrate specificity, stability. Computational approaches use molecular modeling, docking, simulation accurately predict enzyme-substrate interactions enhance chitinase variants' design. Integrating multidisciplinary enables development highly efficient tailored specific applications. This summarizes knowledge advances engineering serve indispensable guideline researchers industrialists seeking uses.
Language: Английский
Citations
1
Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(45), P. 24928 - 24943
Published: Nov. 4, 2024
In the field of food processing, enzymes play a pivotal role in improving product quality and flavor, extending shelf life. However, exposure traditional to high temperatures during processing often leads decrease activity or even inactivation, limiting effectiveness their application under high-temperature conditions. Therefore, modification thermostability adapt extreme conditions through protein engineering has become key way improve efficiency economic benefits industrial production. Directed evolution semirational design strategies laboratory have proven be broadly applicable frameworks for biochemical researchers field, including those who are beginners. this review, we systematically summarize high-throughput screening strategies, introduce some intuitive computer simulation software enzyme enzymes. The these techniques provides comprehensive guide optimization addition, latest hot topics genetically engineered discussed.
Language: Английский
Citations
1
Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(47), P. 26404 - 26415
Published: Nov. 13, 2024
In this study, the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and multicopper oxidase (MCO) of
Language: Английский
Citations
1
SynBio, Journal Year: 2024, Volume and Issue: 2(2), P. 142 - 173
Published: April 7, 2024
Genetic diversity is the foundation of evolutionary resilience, adaptive potential, and flourishing vitality living organisms, serving as cornerstone for robust ecosystems continuous evolution life on Earth. The landscape directed evolution, a powerful biotechnological tool inspired by natural processes, has undergone transformative shift propelled innovative strategies generating genetic diversity. This fuelled several factors, encompassing utilization advanced toolkits like CRISPR-Cas base editors, enhanced comprehension biological mechanisms, cost-effective custom oligo pool synthesis, seamless integration artificial intelligence automation. comprehensive review looks into myriad methodologies employed constructing gene libraries, both in vitro vivo, categorized three major classes: random mutagenesis, focused DNA recombination. objectives this are threefold: firstly, to present panoramic overview recent advances creation; secondly, inspire novel ideas further innovation generation; thirdly, provide valuable resource individuals entering field evolution.
Language: Английский
Citations
0
Journal of Biological Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 107992 - 107992
Published: Nov. 1, 2024
The development of enzymes with high-temperature resistance up to 100 °C is significant and practical value in advancing the sustainability industrial production. Phytase, a crucial enzyme feed applications, encounters challenges due its limited heat resistance. Herein, we employed rational design strategies involving introduction disulfide bonds, free energy calculation, B-factor analysis based on crystal structure phytase APPAmut4 (1.90 Å), variant enhanced expression levels derived from Yersinia intermedia, improve thermostability. Among 144 variants experimentally verified, 29 exhibited significantly improved thermostability higher t
Language: Английский
Citations
0