Functional characterization of an epsilon glutathione S-transferase (SfGSTe9) associated with insecticide detoxification in Spodoptera frugiperda

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 208, P. 106305 - 106305

Published: Jan. 21, 2025

Language: Английский

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The expansion and loss of specific olfactory genes in relatives of parasitic lice, the stored-product psocids (Psocodea: Liposcelididae)

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: Jan. 15, 2025

Booklice, belonging to the genus Liposcelis (Psocodea: Liposcelididae), commonly known as psocids, infest a wide range of stored products and are implicated in transmission harmful microorganisms such fungi bacteria. The olfactory system is critical for insect feeding reproduction. Elucidating molecular mechanisms booklice crucial developing effective control strategies. In this study, we aim bridge knowledge gap by leveraging transcriptome genome data from five species. Using HMMER method manual annotation, have identified common gene families associated with processes, including odorant binding proteins (OBPs), chemosensory (CSPs), receptors (ORs), ionotropic (IRs), sensory neuron membrane (SNMPs). Specifically, 94, 118, 26, 47, 34 olfactory-related genes L. bostrychophila, tricolor, entomophila, decolor, yangi, respectively. Comparison quantities revealed that number ORs IRs significantly higher than those transcriptome. This discrepancy may be due specific expression these certain tissues or their lack during experimental stage. Simultaneously, analysis profiles across different developmental stages varying periods peak genes. These results suggest identification on genome-wide scale more feasible reliable using transcriptome-based approach. Additionally, compared parasitic lice, possess increase inability lice survive without host, whereas habits live complex variable environments. Furthermore, observed IR family tricolor has undergone degree amplification, which facilitate its adaptation diverse environmental conditions. We species first time, providing valuable insights future functional investigations into pheromone recognition. discoveries present promising targets effectively managing psocid pests.

Language: Английский

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Ultrastructure and Transcriptome Analysis of the Larval Integument in Solitary and Gregarious Phases of Mythimna separata

Insects, Journal Year: 2025, Volume and Issue: 16(2), P. 190 - 190

Published: Feb. 10, 2025

Mythimna separata larvae exhibit both solitary and gregarious phases under low high population density, respectively; furthermore, differences in morphology, behavior physiology have been observed the two phases. The integument plays an essential role fitness, general metabolism, communication, survival of insects; however, ultrastructure gene expression are largely unknown. In this study, was compared, transcriptome analysis conducted to identify which genes were differentially expressed results showed that had thicker integuments more polygonal particles on cuticle surface than larvae. Using Illumina HiSeq™ sequencing platform, 2774 (DEGs) generated. Among these, many transcripts identified with roles synthesis fatty acids; structural components insecticide detoxification larval qRT-PCR used validate patterns selected transcripts. This study provides a valuable resource for understanding molecular basis behavioral physiological M. separata.

Language: Английский

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Chromosome‐level genome of the parthenogenetic booklouse Liposcelis bostrychophila reveals high heterozygosity and a nonhomologous chromosome

Insect Science, Journal Year: 2024, Volume and Issue: unknown

Published: June 6, 2024

Abstract Psocodean species are emerging as significant sanitary and stored‐product pests, posing threats to human health global food security. Out of an estimated 10 000 species, the whole genome sequences only 4 have been published. Genomic resources crucial for establishing effective pest control enhancing our understanding evolution psocodean species. In this study, we employed Illumina PacBio sequencing along with Hi‐C scaffolding techniques generate a chromosome‐level assembly parthenogenetic booklouse Liposcelis bostrychophila . The assembled measures 291.67 Mb in length comprises 9 chromosomes. Notably, L. exhibits high level heterozygosity features distinctive nonhomologous chromosome. This heterozygous characteristic may arise from mutation rates, based on genomic variations analysis across multiple generations. Our revealed significantly expanded gene families, primarily associated detoxification feeding habits These include integument esterases (ESTs), ATP‐binding cassette (ABC) transporter genes gustatory receptors (GRs). high‐quality sequence provides valuable further study molecular mechanisms stress resistance. It enables researchers identify functional facilitates research population genetics, phylogeny booklice.

Language: Английский

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Characterization of Four Carboxylesterases Involved in Detoxification of β-Cypermethrin, λ-Cyhalothrin, and Malathion in Helicoverpa armigera

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(46), P. 25533 - 25548

Published: Nov. 7, 2024

Hübner (Lepidoptera: Noctuidae) is a globally devastating pest and has evolved with varying levels of resistance to broader spectrum insecticides. CarEs are major enzymes involved in insecticide detoxification metabolic resistance. Four

Language: Английский

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