Biochemical characterization and biocatalytic application of a hyperthermostable tagatose 4-epimerase from Infirmifilum uzonense

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 305, P. 141168 - 141168

Published: Feb. 17, 2025

Language: Английский

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High-Temperature Catalytic Platform Powered by Thermophilic Microorganisms and Thermozymes

Synthetic biology and engineering, Journal Year: 2025, Volume and Issue: 3(1), P. 10001 - 10001

Published: Jan. 1, 2025

Language: Английский

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Transforming monosaccharides: recent advances in rare sugar production and future exploration

BBA Advances, Journal Year: 2025, Volume and Issue: 7, P. 100143 - 100143

Published: Jan. 1, 2025

Language: Английский

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Discovery, design, and engineering of enzymes based on molecular retrobiosynthesis

mLife, Journal Year: 2025, Volume and Issue: 4(2), P. 107 - 125

Published: March 28, 2025

Abstract Biosynthesis—a process utilizing biological systems to synthesize chemical compounds—has emerged as a revolutionary solution 21st‐century challenges due its environmental sustainability, scalability, and high stereoselectivity regioselectivity. Recent advancements in artificial intelligence (AI) are accelerating biosynthesis by enabling intelligent design, construction, optimization of enzymatic reactions systems. We first introduce the molecular retrosynthesis route planning biochemical pathway including single‐step algorithms AI‐based design tools. highlight advantages large language models addressing sparsity data. Furthermore, we review enzyme discovery methods based on sequence structure alignment techniques. Breakthroughs structural prediction expected significantly improve accuracy discovery. also summarize for de novo generation nonnatural or orphan reactions, focusing functional annotation techniques reaction small molecule similarity. Turning engineering, discuss strategies thermostability, solubility, activity, well applications AI these fields. The shift from traditional experiment‐driven data‐driven computationally driven is already underway. Finally, present potential provide perspective future research directions. envision expanded biocatalysis drug development, green chemistry, complex synthesis.

Language: Английский

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The use of isomerases and epimerases for the production of the functional sugars mannose, allulose and tagatose from Fructose

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(4)

Published: April 1, 2025

Language: Английский

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Biosynthesis of a healthy natural sugar D-tagatose: advances and opportunities

Critical Reviews in Biotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 16

Published: April 23, 2025

D-tagatose is a natural low-calorie rare sugar with nearly the same sweet taste as sucrose. It has nutritional and functional properties of great interest for health, such anti-diabetes, anti-caries, anti-atherosclerosis, anti-hyperlipidemia, anti-aging, improvement intestinal microflora, etc. The production from D-galactose catalyzed by an alkali suffers limited supplies costly feedstock (i.e., lactose) high manufacturing costs due to harsh reaction conditions, separation, well severe degradation pollution. In this review, we briefly present its physiological effects, review recent advances in biosynthesis inexpensive abundant glucans (e.g., starch) their derivatives D-glucose D-fructose) lactose, including both academic literature industrial patents, discuss future challenges opportunities. can be four types biocatalysts: enzymes, whole-cells, microbial fermentation, vitro multi-enzyme molecular machines. biomanufacturing starchy machines could most promising approach because it not only makes ample starch but also surpasses equilibria monosaccharide isomerization reactions D-fructose-to-D-tagatose, D-galactose-to-D-tagatose). filler variety food drinks or key component mixed other sweeteners would become predominant starch-derived sweetener partially replace high-fructose corn sirup future.

Language: Английский

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Characterization of a tagatose 4-epimerase and optimizing its expression for efficient D-tagatose bioconversion from fructose

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 311, P. 143722 - 143722

Published: April 30, 2025

Language: Английский

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Mining and identifying a D-mannose isomerase with high fructose isomerization activity and its expression in Bacillus subtilis for D-mannose production

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 311, P. 143724 - 143724

Published: May 1, 2025

Language: Английский

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Protein Engineering of Tagatose 4-Epimerase for D-Tagatose Production

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 11, 2025

D-Tagatose, a promising sugar substitute with various functional properties and commercial applications, can be enzymatically converted from D-fructose by tagatose 4-epimerase. The development of an efficient 4-epimerase that catalyzes the conversion into D-tagatose is essential to make production technology applicable. In this study, Thermotogae (TsT4Ease) was engineered through semi-rational design directed evolution, resulting in 2.8-fold improvement catalytic activity compared wild type (WT). reached 42 g/L 2 h. Crystal structure analysis determined structural features common (α/β)8-TIM barrel Zn2+-binding architecture at active center. Subsequent molecular dynamics (MD) simulations revealed substitutions improved substrate binding energy stabilized pocket. This study offers new insights structure-function relationship TsT4Ease provides candidate

Language: Английский

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Enhanced thermostability and catalytic activity for arginine deiminase from Enterobacter faecalis SK32.001 via combinatorial mutagenesis

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 138004 - 138004

Published: Nov. 1, 2024

Language: Английский

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