Assessing Microbial Metabolic and Biological Diversity to Inform Natural Product Library Assembly DOI

Victoria M. Anderson,

Karen Wendt,

James B. Caughron

et al.

Journal of Natural Products, Journal Year: 2022, Volume and Issue: 85(4), P. 1079 - 1088

Published: April 13, 2022

The pressing need for novel chemical matter to support bioactive compound discovery has led natural product researchers explore a wide range of source organisms and environments. One the implicit guiding principles behind those efforts is notion that sampling different environments critical accessing unique products. This idea was tested by comparing fungi from disparate biomes: aquatic sediments Lake Michigan (USA) terrestrial samples taken surrounding soils. Matched sets Penicillium brevicompactum, expansum, oxalicum two were compared, revealing modest differences in physiological performance output. Analysis LC-MS/MS-derived molecular feature data showed no source-dependent richness. High levels scaffold homogeneity also observed with 78–83% scaffolds shared among spp. isolates. A comparison culturable biomes indicated certain genera more strongly associated (e.g., Trichoderma, Pseudeurotium, Cladosporium, Preussia) versus environment Fusarium, Pseudogymnoascus, Humicola, Acremonium). Taken together, these results suggest focusing on microbial resources are an may have pronounced effect enhancing sought-after diversity needed screening collections.

Language: Английский

Fungal–fungal co-culture: a primer for generating chemical diversity DOI Creative Commons
Sonja L. Knowles, Huzefa A. Raja, Christopher D. Roberts

et al.

Natural Product Reports, Journal Year: 2022, Volume and Issue: 39(8), P. 1557 - 1573

Published: Jan. 1, 2022

Covering: 2002 to 2020In their natural environment, fungi must compete for resources. It has been hypothesized that this competition likely induces the biosynthesis of secondary metabolites defence. In a quest discover new chemical diversity from fungal cultures, growing trend recapitulate competitive environment in laboratory, essentially co-culture. This review covers fungal-fungal co-culture studies beginning with first literature report 2002. Since then, there number reported as result studies. Specifically, discusses and provides insights into (1) rationale pairing strains, (2) ways grow co-culture, (3) different approaches screening co-cultures diversity, (4) determining metabolite-producing strain, (5) final thoughts regarding approach. Our goal is provide set practical strategies generate unique products research community can utilize.

Language: Английский

Citations

73
Freshwater fungi from karst landscapes in China and Thailand DOI
Jing Yang, Lingling Liu, E.B. Gareth Jones

et al.

Fungal Diversity, Journal Year: 2023, Volume and Issue: 119(1), P. 1 - 212

Published: March 1, 2023

Language: Английский

Citations

52
Predicting global numbers of teleomorphic ascomycetes DOI Creative Commons
Indunil C. Senanayake, Dhandevi Pem, Achala R. Rathnayaka

et al.

Fungal Diversity, Journal Year: 2022, Volume and Issue: 114(1), P. 237 - 278

Published: Feb. 17, 2022

Abstract Sexual reproduction is the basic way to form high genetic diversity and it beneficial in evolution speciation of fungi. The global teleomorphic species Ascomycota has not been estimated. This paper estimates number for sexual ascomycetes based on five different estimation approaches, viz. by numbers described fungi, fungus:substrate ratio, ecological distribution, meta-DNA barcoding or culture-independent studies previous Ascomycota. assumptions were made with currently most accepted, “2.2–3.8 million” estimate results concluding that 90% reproduce sexually. Catalogue Life, Species Fungorum published research used data procurement. average value from all methods 1.86 million, ranging 1.37 2.56 million. However, only around 83,000 have deposited repositories. ratio between predicted 1:22. Therefore, where are undiscovered ascomycetes? undescribed no doubt be found biodiversity hot spots, poorly-studied areas complexes. Other poorly studied niches include extremophiles, lichenicolous human pathogens, marine fungicolous Undescribed present unexamined collections specimen repositories incompletely earlier species. Nomenclatural issues, such as use separate names teleomorph anamorphs, synonyms, conspecific names, illegitimate invalid also affect Interspecies introgression new species, while reduced extinctions.

Language: Английский

Citations

32
Fungal Diversity and Community Composition across Ecosystems DOI Creative Commons
Pavla Debeljak, Federico Baltar

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(5), P. 510 - 510

Published: April 25, 2023

Fungi have shaped the biosphere since development of life on Earth. Despite fungi being present in all environments, most available fungal research has focused soils. As a result, role and composition communities aquatic (marine freshwater) environments remain largely unexplored. The use different primers to characterise additionally complicated intercomparisons among studies. Consequently, we lack basic global assessment diversity across major ecosystems. Here, took advantage recently published 18S rRNA dataset comprising samples from ecosystems (terrestrial, freshwater, marine) attempt community composition. We found highest diversities for terrestrial > freshwater marine pronounced gradients along temperature, salinity, latitude also identified abundant taxa each these ecosystems, mostly dominated by Ascomycota Basidiomycota, except rivers where Chytridiomycota dominated. Collectively, our analysis provides environmental highlighting distinct order ASVs (amplicon sequencing variants) ecosystem, thus filling critical gap study Earth’s mycobiome.

Language: Английский

Citations

22
New insights into freshwater ascomycetes: discovery of novel species in diverse aquatic habitats DOI Creative Commons
Lu Li, D. Jayarama Bhat, Hong-Bo Jiang

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 14

Published: Jan. 13, 2025

During investigations of freshwater fungi in Hunan and Yunnan provinces, China, Chaetopsina yunnanensis sp. nov. (Nectriaceae), Parafuscosporella hunanensis (Fuscosporellaceae), Pleurotheciella (Pleurotheciaceae) were discovered on submerged decaying wood branches. Based phylogenetic analyses, C. formed a separate branch with pinicola nested among other species Nectriaceae (Hypocreales). Furthermore, hitherto known beijingensis shared the same fulva, type genus, demonstrating their conspecific status. Therefore, is formally synonymized under an amended circumscription. Pa. well-separated subclade ex-type strain mucosa clustered within Fuscosporellaceae (Fuscosporellales). In addition, genus treated as distinct from Vanakripa due to lack evidence clarifying congeneric status latter. Pl. found be sister saprophytica, forming dimorphospora Pleurotheciaceae (Pleurotheciales). Morphologically, fits well generic concept holomorphic state hyphomycetous asexual morph producing pigmented, setiform conidiophores, phialidic conidiogenous cells, hyaline conidia, nectria-like sexual morph. having acrogenous, apiosporous, versicolored, obovoid obpyriform conidia. contrast, resembles dimorphism two types conidia (Type I, brown, muriform/phragmosporous conidia; Type II, hyaline, amerosporous/didymorsporous conidia). The novelty taxa explained detailed descriptions, photo-micrographic illustrations, polymorphism, multigene analyses Bayesian inference maximum likelihood criteria.

Language: Английский

Citations

1
Oxic–anoxic cycling promotes coupling between complex carbon metabolism and denitrification in woodchip bioreactors DOI
Philip M. McGuire, Natalie Butkevich, Aryaman Saksena

et al.

Environmental Microbiology, Journal Year: 2023, Volume and Issue: 25(9), P. 1696 - 1712

Published: April 27, 2023

Denitrifying woodchip bioreactors (WBRs) are increasingly used to manage the release of non-point source nitrogen (N) by stimulating microbial denitrification. Woodchips serve as a renewable organic carbon (C) source, yet recalcitrance C in lignocellulosic biomass causes many WBRs be C-limited. Prior studies have observed that oxic-anoxic cycling increased mobilization C, nitrate (NO3- ) removal rates, and attenuated production nitrous oxide (N2 O). Here, we use multi-omics approaches amplicon sequencing fungal 5.8S-ITS2 prokaryotic 16S rRNA genes elucidate drivers for enhanced NO3- N2 O under redox-dynamic conditions. Transient oxic periods stimulated expression ligninolytic enzymes, increasing bioavailability woodchip-derived denitrification genes. Nitrous reductase (nosZ) were primarily clade II, ratio II/clade I nosZ transcripts during transition was strongly correlated with yield. Analysis metagenome-assembled genomes revealed denitrifying microorganisms also genotypic ability degrade complex polysaccharides like cellulose hemicellulose, highlighting adaptation WBR microbiome ecophysiological niche matrix.

Language: Английский

Citations

16
Ecosystem services provided by fungi in freshwaters: a wake-up call DOI
Sahadevan Seena, Christiane Baschien, Juliana Barros

et al.

Hydrobiologia, Journal Year: 2022, Volume and Issue: 850(12-13), P. 2779 - 2794

Published: Oct. 19, 2022

Language: Английский

Citations

18
From treetops to river bottoms: Exploring the role of phyllosphere fungi in aquatic fungal communities DOI
Clifford A. Ochs, Michaela Hayer,

Egbert Schwartz

et al.

Ecology, Journal Year: 2025, Volume and Issue: 106(1)

Published: Jan. 1, 2025

Abstract Fungi play a crucial role in aquatic leaf litter decomposition. Aquatic fungi have long been thought to spend the majority of their lives water. Here, we explore possibility an amphibious life cycle, where phyllosphere part cycle systems. We used internal transcribed spacer (ITS) fungal sequencing follow onto submerged litter, and quantitative stable isotope probing (qSIP) differentiate active inactive fungi. found that around 30% on entered stream with these were as active, if not more than, colonizing from water column. These results demonstrate are important communities.

Language: Английский

Citations

0
New Antimicrobial Cyclodepsipeptides from a Freshwater Fungus from the Sierra Madre Oriental in Mexico DOI Creative Commons
Itzel Rubí Yeverino, T. Sosa, Laura Aguilar-Vega

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: 10(5), P. 5087 - 5096

Published: Jan. 29, 2025

The Sierra Madre Oriental (SMO) in Mexico is a complex, unexplored geological area with multiple habitats and unique physical, chemical, biological features. A bioactive-guided study of the organic extract from solid-state fermentation culture taxonomically unidentified fungus isolated submerged wood waterfall "El Caracol", Nuevo Leon, at SMO, led to identification three new cyclodepsipeptides (1–3) known Sch 217048 (4) 378161 (5). Structures all compounds were elucidated by spectroscopic spectrometric methods. 4 5 showed antimicrobial activity against Gram-positive strains Gram-negative Acinetobacter baumannii ATCC 17978, including multidrug-resistant clinical strain A564. In addition, no toxic Galleria mellonella larvae model. Finally, molecular networking analysis allowed us annotate network. This first systematic chemical SMO Mexico.

Language: Английский

Citations

0
Environmental yeasts differentially impact the development and oviposition behavior of the Asian tiger mosquito Aedes albopictus DOI Creative Commons

Simon Malassigné,

Mathieu Laÿs,

Laurent Vallon

et al.

Microbiome, Journal Year: 2025, Volume and Issue: 13(1)

Published: April 16, 2025

While the Asian tiger mosquito (Aedes albopictus), a known vector of many arboviruses, establishes symbiotic associations with environmentally acquired yeasts, their impact on biology remains poorly investigated. To better understand these associations, we hypothesized that waterborne yeasts colonizing larval gut differentially support development based capacity to produce riboflavin or recycle nitrogen waste into proteins by secreting uricase, as B vitamins and amino acids are crucial for development. address this hypothesis, used axenic gnotobiotic insects gauge specific different environmental Ae. albopictus survival. We then evaluated whether observed variations across yeast species could be linked differential uricolytic activities varying quantities in insecta. Finally, given oviposition site selection favors conditions enhance offspring performance, tested promote faster mediate gravid females. Differences times were used. Yeasts like Rhodotorula mucilaginosa Aureobasidium pullulans promoted rapid associated improved Conversely, such Torulaspora delbrueckii Martiniozyma asiatica, which led slower development, produced smaller adults. Notably, R. mucilaginosa, fastest provided high intakes nitrogenous recycling protein synthesis through strong uricolytic-ureolytic activity. Behavioral experiments indicated promoting "attract Our findings highlight set present natural breeding sites can survival enhancing nutritional intake, thereby attracting Variations time likely levels production capacities among species. This study opens new perspectives trophic interactions between mosquitoes mycobiota, emphasizing importance nitrogen-containing molecules essential acids, proteins, mycobiota. Video Abstract.

Language: Английский

Citations

0