Molecular insights into the phase transition of lysozyme into amyloid nanostructures: Implications of therapeutic strategies in diverse pathological conditions

Advances in Colloid and Interface Science, Journal Year: 2024, Volume and Issue: 331, P. 103205 - 103205

Published: May 23, 2024

Language: Английский

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Characterization of insulin and bile acid complexes in liposome by different mass spectrometry techniques

Analytical and Bioanalytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Language: Английский

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Raubasine-Induced Groove Binding in Salmon Testes DNA: Exploring the Structural Modulation, Antiglycation, and Antioxidant Properties

The Journal of Physical Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

As one of nature's most fundamental blueprints and due to its critical role in life processes, DNA has naturally become the cornerstone numerous research efforts. One particularly intriguing area study is understanding how small molecules interact with nucleic acids. In this study, we investigated interaction between plant-derived indole alkaloid Raubasine (Ajmalicine; AJM) Salmon Testes (ST) using biophysical computational techniques. A hyperchromic shift fluorescence intensity indicated effective binding AJM ST DNA. The constant was order 105 M–1 a single preferential mode. Thermodynamic analysis revealed that exothermic driven by positive entropy negative enthalpy. salt-dependent indicates involvement nonpolyelectrolytic forces interaction. Studies iodide quenching, urea denaturation, dye displacement, molecular docking further support binds through groove binding. Structural perturbation evident from circular dichroism. stability AJM–DNA complex confirmed dynamics simulations. Prolonged elevated blood glucose levels induce nonenzymatic glycation DNA, resulting DNA–AGE (advanced end-products) formation free radical production, which disrupts structure. We explored ST-DNA suppression AJM. DNA–AGEs vitro were characterized UV–vis spectroscopy. inhibition assessed changes AGEs intensity, gel electrophoresis patterns, antioxidant activity, highlighting ability target glycated sites or neutralize radicals generated during glycation. Our findings reveal AJM's potential prevent AGEs, may offer promising avenues for targeted therapies against glycation-related diseases such as diabetes, neurodegeneration, cancer.

Language: Английский

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Probing the Molecular Interactions of Piperidine Alkaloid Lobeline with Ovalbumin: Anti-Aggregation and Anti-Glycation Potential

The Journal of Physical Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: May 5, 2025

Lobeline (LOB) is a bioactive alkaloid known for its neuroprotective and cognitive-enhancing properties, particularly in mitigating oxidative stress inflammation associated with neurodegenerative diseases. While LOB has been studied pharmacological roles CNS disorders, substance abuse treatment, smoking cessation, molecular interactions proteins potential antiamyloid, antiglycation, antioxidant properties remain largely unexplored. This study addresses this gap by analyzing ovalbumin (OVA) through advanced biophysical computational techniques to elucidate therapeutic inform drug development. Fluorescence spectroscopy confirmed static quenching mechanism binding constant (Kb) of 105 M-1 monopreferential site OVA LOB. Isothermal titration calorimetry (ITC) indicated an exothermic, entropy-driven interaction, while differential scanning (DSC) demonstrated LOB-induced stabilization OVA. Synchronous fluorescence red edge excitation shift highlighted LOB's effect on Trp Tyr residues, circular dichroism (CD) conformational changes Molecular modeling (docking dynamic simulation) corroborated the experimental findings. Turbidity, thioflavin T (ThT), nile (NR), 8-anilinonaphthalene-1-sulfonic acid (ANS), CD, morphological studies fibril formation, aggregation was shown be inhibited ligand vitro. Additionally, d-fructose used glycate protein, creating model examine antiglycation effects These aspects are essential advancing targeted design delivery systems, as they provide vital insights into modern biochemical research.

Language: Английский

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Inhibition of amyloidal aggregation of insulin by amino acid conjugated bile acids: An insight into the possible role of biosurfactants in modulating the fibrillation kinetics and cytotoxicity

Journal of Molecular Liquids, Journal Year: 2024, Volume and Issue: 397, P. 124142 - 124142

Published: Feb. 1, 2024

Language: Английский

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Modulation of AIE and Intramolecular Charge Transfer of a Pyrene-Based Probe for Discriminatory Detection and Imaging of Oligomers and Amyloid Fibrils

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 18, 2024

Oligomers and amyloid fibrils formed at different stages of protein aggregation are important biomarkers for a variety neurodegenerative diseases including Alzheimer's Parkinson's diseases. The development probes the sensitive detection oligomeric species is early stage diagnosis amyloidogenic Many small molecular dyes have been developed to probe dynamic growth fibrils. However, there lack discriminatory strategies monitor dynamics both oligomers based on differential modulation photophysical properties single dye. Here we report pyrene-based intramolecular charge transfer (ICT) dye with large Stokes shifted red-emitting induced emission (AIE) monitoring populations during hen egg white lysozyme (HEWL) protein. At aggregation, accumulation HEWL results in rapid substantial increase red AIE intensity 660 nm. Later, as transform into mature fibrils, exhibits distinct change. Binding strongly suppresses enhances ICT emission. This evidenced by gradual decrease (∼660 nm) an LE (∼490 (∼540 intensities later aggregation. Thus, provides simultaneous measurements population also enables imaging simultaneously using channels super-resolution confocal fluorescence microscopy.

Language: Английский

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Furosemide Derails Human Lysozyme Fibrillation by Interacting with Aggregation Hot Spots: A Biophysical Comprehension

The Journal of Physical Chemistry B, Journal Year: 2024, Volume and Issue: 128(18), P. 4283 - 4300

Published: April 29, 2024

Kidney-associated human lysozyme amyloidosis leads to renal impairments;thus, patients are often prescribed furosemide. Based on this fact, the effect of furosemide induced fibrillation, in vitro, is evaluated by spectroscopic, calorimetric, computational, and cellular-based assays/methods. Results show that increases lag phase decreases apparent rate aggregation lysozyme, thereby decelerating nucleation amyloid fibril formation, as confirmed decrease level Thioflavin-T fluorescence. Fewer entities hydrodynamic radii ∼171 nm instead fibrils (∼412 nm) detected presence dynamic light scattering. Moreover, extent conversion α/β structure into a predominant β-sheet. The isothermal titration calorimetry established forms complex with which was also through fluorescence quenching computational studies. Also, lytic activity inhibited competitively due involvement amino acid residues active site catalysis, well formation. Conclusively, interacts Gln58, Ile59, Asn60, Ala108, Trp109 aggregation-prone regions 2 4 masking its sites generating only lower-order less toxic red blood cells than fibrils. Thus, slows progression fibrillation lysozyme.

Language: Английский

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Mechanistic exploration of Perfluorooctanoic Acid-Induced Hen egg white lysozyme aggregation at physiological Conditions: Spectroscopic and thermodynamic insights

Journal of Molecular Liquids, Journal Year: 2024, Volume and Issue: 408, P. 125255 - 125255

Published: Aug. 1, 2024

Language: Английский

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Ankaflavin and Monascin Prevent Fibrillogenesis of Hen Egg White Lysozyme: Focus on Noncovalent and Covalent Interactions

The Journal of Physical Chemistry B, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 5, 2024

Misfolding and amyloid fibrillogenesis of proteins have close relationships with several neurodegenerative diseases. The present work investigates the inhibitive activities ankaflavin (AK) monascin (MS), two yellow pigments separated from

Language: Английский

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Impact of yohimbine on myoglobin stability: insights from molecular spectroscopic, and computational approaches

Journal of Biomolecular Structure and Dynamics, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 13

Published: Nov. 25, 2024

The prolific role of bioactive ligands in interacting with a variety proteins has become focal point interest pharmacokinetics and pharmacodynamics, thus sparking substantial enthusiasm within the realm medicinal chemistry. reversible binding small molecules is characteristic feature, it's essential to investigate these interactions understand their mode mechanism action human body. Therefore, primary objective present study underlying by which yohimbine (Yoh) interacts protein myoglobin (Mb), employing both

Language: Английский

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