High-low temperature switch induces formation of catalytic and cytotoxic papain amyloid aggregates

Food Chemistry, Journal Year: 2025, Volume and Issue: 479, P. 143657 - 143657

Published: March 1, 2025

Language: Английский

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Cold Plasma-Induced Modulation of Protein and Lipid Macromolecules: A Review

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1564 - 1564

Published: Feb. 13, 2025

Nowadays, the food industry is prioritizing many innovative processing technologies that can produce minimally processed foods with superior and higher quality, lower costs, faster operations. Among these advancements, cold plasma (CP) stands out for its remarkable capabilities in preservation extending shelf life. Beyond established role microbial inactivation, CP has emerged as a transformative tool modifying biomolecules through reactive species, addressing versatile requirements of industries various applications. This review focuses on interactions between species essential macromolecules, including proteins, lipids, polysaccharides. The novelty lies detailed examination how technology triggers structural, functional, biochemical changes proteins lipids explains mechanisms involved. It connects fundamental molecular transformations to practical applications, such enhanced protein functionality, lipid stabilization, improved oxidative resistance. induces alterations structure, especially amino acid configurations, be applicable formulation advanced gel, 3D printing, thermostable emulsions, solubility, sensory materials. explores ability modify allergenicity, different effects mechanical interfacial properties production trans-fat-free oils. Despite potential, understanding mechanism CP's macromolecules also discussed. Furthermore, this addresses key challenges outlines future research opportunities, positioning sustainable adaptable approach innovating next-generation systems. Further crucial fully understand potential processing, followed by product development.

Language: Английский

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Targeting Lysozyme-Linked Amyloidosis through Piperine-Functionalized Gold Nanoparticles

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Excess accumulation of misfolded and mutated human lysozyme (HuL) is the pathological hallmark non-neuropathic systemic amyloidosis. These deposits are rich in cross β-sheet conformers often exist as polymorphic fibrillar structures, which makes it a tricky challenging task to design therapeutic interventions toward HuL-linked amyloidopathy. Here we aimed an effective antiamyloid metal nanoparticle formulation target exposed hydrophobic aggregation-prone stretches HuL. Initially, synthesized characterized piperine-coated gold nanoparticles (AuNPsPip). ThT-probed aggregation studies HuL presence absence AuNPsPip revealed inhibition aggregation. This effect was confirmed through dynamic light scattering (DLS) fluorescence microscopy analyses. We further investigated whether could bind preformed fibrils prevent secondary nucleation process, crucial step amyloidogenesis. Our results showed that not only prevented seed-induced but also disassembled amyloid aggregates, observed with AuNPs or piperine. Experimental computational suggest retention native structure ability interact residues key factors mechanism. The findings this work may aid developing nanoparticle-based formulations pathologies linked

Language: Английский

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Proteomic evidence for amyloidogenic cross-seeding in fibrinaloid microclots

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 17, 2024

Abstract In classical amyloidoses, amyloid fibres form through the nucleation and accretion of protein monomers, with protofibrils fibrils exhibiting a cross-β motif parallel or antiparallel β-sheets oriented perpendicular to fibre direction. These can intertwine mature fibres. Similar phenomena occur in blood from individuals circulating inflammatory molecules (also those originating viruses bacteria). presence inflammagens, pathological clotting occur, that results an anomalous termed fibrinaloid microclots. Previous proteomic analyses these microclots have shown non-fibrin(ogen) proteins, suggesting more complex mechanism than simple entrapment. We provide evidence against entrapment model, noting clot pores are too large centrifugation would removed weakly bound proteins. Instead, we explore whether co-aggregation into may involve axial (multiple proteins within same fibril), lateral (single-protein contributing fibre), both types integration. Our analysis data different diseases shows no significant overlap normal plasma proteome correlation between abundance Notably, abundant like α-2-macroglobulin, fibronectin, transthyretin absent microclots, while less such as adiponectin, periostin, von Willebrand Factor well represented. Using bioinformatic tools including AmyloGram AnuPP, found entrapped exhibit high amyloidogenic tendencies, their integration elements structures. This likely contributes microclots’ resistance proteolysis. findings underscore role cross-seeding microclot formation highlight need for further investigation structural properties implications thrombotic diseases. insights foundation developing novel diagnostic therapeutic strategies targeting disorders.

Language: Английский

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Tailoring the Molecular Structure of 6-Gingerol for Targeting the Phase Separation in Human Lysozyme

The Journal of Physical Chemistry Letters, Journal Year: 2024, Volume and Issue: 15(31), P. 8032 - 8041

Published: July 31, 2024

Human lysozyme undergoes a phase-separation process to form insoluble amyloid-architects that cause several pathologies including systemic amyloidosis. Here we have tailored 6-gingerol by extending its molecular framework with active functional groups specifically target phase-transition events. Aggregation assay revealed 4-aromatic moieties (MTV4) substantially suppressed the conversion of low-density liquid phase (LDLP) solid-phase structured amyloids. The data obtained from biophysical, computational, and microscopic imaging tools suggest direct intervention MTV4 liquid–liquid separation. CD was able retain native conformation lysozyme. Both biomolecular computational reveal interference aggregation-prone hydrophobic stretches within lysozyme, thereby retaining structure reversing misfolded intermediates monomers. Also, induce rapid dissolution preformed-toxic amyloid fibrils. These results reinforce importance aromatic–aromatic interaction in preventing human

Language: Английский

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Proteomic Evidence for Amyloidogenic Cross-Seeding in Fibrinaloid Microclots

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10809 - 10809

Published: Oct. 8, 2024

In classical amyloidoses, amyloid fibres form through the nucleation and accretion of protein monomers, with protofibrils fibrils exhibiting a cross-β motif parallel or antiparallel β-sheets oriented perpendicular to fibre direction. These can intertwine mature fibres. Similar phenomena occur in blood from individuals circulating inflammatory molecules (and also some originating viruses bacteria). Such pathological clotting result an anomalous termed fibrinaloid microclots. Previous proteomic analyses these microclots have shown presence non-fibrin(ogen) proteins, suggesting more complex mechanism than simple entrapment. We thus provide evidence against such entrapment model, noting that clot pores are too large centrifugation would removed weakly bound proteins. Instead, we explore whether co-aggregation into may involve axial (multiple proteins within same fibril), lateral (single-protein contributing fibre), both types integration. Our analysis data different diseases shows no significant quantitative overlap normal plasma proteome correlation between abundance their Notably, abundant like α-2-macroglobulin, fibronectin, transthyretin absent microclots, while less as adiponectin, periostin, von Willebrand factor well represented. Using bioinformatic tools, including AmyloGram AnuPP, found entrapped exhibit high amyloidogenic tendencies, integration elements structures. This likely contributes microclots’ resistance proteolysis. findings underscore role cross-seeding microclot formation highlight need for further investigation structural properties implications thrombotic diseases. insights foundation developing novel diagnostic therapeutic strategies targeting disorders.

Language: Английский

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Leverage of lysozyme dietary supplementation on gut health, hematological, antioxidant, and immune parameters in different plumage-colors Japanese quails

Poultry Science, Journal Year: 2024, Volume and Issue: 104(1), P. 104474 - 104474

Published: Nov. 4, 2024

The current study was conducted on two different feather-colored Japanese quail varieties (brown and white) to examine the impact of lysozyme (LZ) dietary supplementation growth performance, hematological profile, serum lysozyme, phagocytic antioxidant activities, along with gut status relative expression some antioxidant- immune-related genes. Two forms LZ; extracted from egg white (natural LZ (NLZ)), commercial (CLZ) were included in this experiment. For each variety, 240 birds randomly allocated into four groups replicates per group. first group (control) ate basal diet (BD) only. other BD supplemented (CLZ, at 100 mg/kg diet), NLZ (NLZ1) 200 (NLZ2) diet. Different treatments differentially modulated quail's performance significant increases final body weight white-feathered quails fed NLZ1 compared treatments. NLZ2 CLZ noticeably increased total activity (TA) white- brown-feathered quails, respectively. Also, all displayed distinct activities. status, both exhibited intestinal villi length goblet cell count reductions lactobacillus, coliform, bacterial counts. These effects linked marked modulations SOD, CAT, GPX, andIL-1βgene levels varieties. Therefore, could growth, immune as well health.

Language: Английский

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Detoxification of Peptide-Containing Biotoxins

Journal of NBC Protection Corps, Journal Year: 2024, Volume and Issue: 8(3), P. 256 - 269

Published: Nov. 20, 2024

Highlights. Peptide biotoxins are important problem for human health and as lethal agents due to their wild diversity of chemical structures biological sources. Such peptide prion proteins can be effectively neutralized by different methods, including protease treatment. Relevance – toxins containing peptides possess serious danger life well being humans. There a lot reviews summarizing immunologic protective measures against these toxins. As opposed that an enzymatic detoxification is, at best, considered superficially. The purpose the work is analysis main up-to-date trends development remedies nature. source base research mainly English–language scientific literature available via global Internet network, authors' own published experimental studies. method analytical. Results. Currently efficiency detoxifying immunological drugs surging highly productive methods screening selection effective clones producing monoclonal antibodies. Special attention in review paid application hydrolytic enzymes which alternative immunobiological during detoxication biotoxins. natural analogue system "toxin–antitoxin" procaryotes. More than four types inhibitors know: blocking catalytic activity; hindering target receptors; inhibiting toxin acting on its structure; allosterically modulating biotoxin activity. encouraging data neutralization prions soils treatment complication. Conclusions. Application proteases could viable agents.

Language: Английский

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Enhancing the Stability and Anticancer Activity of Escherichia coli Asparaginase Through Nanoparticle Immobilization: A Biotechnological Perspective on Nano Chitosan

Polymers, Journal Year: 2024, Volume and Issue: 16(23), P. 3260 - 3260

Published: Nov. 23, 2024

There is a shortage in the experimental research directly comparing effectiveness of different nanoparticles boosting asparaginase (ASNase) activity. This study assessed impact various on enhancing ASNase activity, stability, and anticancer effects through immobilization.

Language: Английский

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Thyroid Malignancy and Cutaneous Lichen Amyloidosis: Key Points Amid RET Pathogenic Variants in Medullary Thyroid Cancer/Multiple Endocrine Neoplasia Type 2 (MEN2)

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 9765 - 9765

Published: Sept. 10, 2024

We aimed to provide an updated narrative review with respect the RET pathogenic variants and their implications at clinical molecular level in diagnosis of medullary thyroid cancer (MTC)/multiple endocrine neoplasia (MEN) type 2, particularly presence cutaneous lichen amyloidosis (CLA). searched English-language, extenso original articles no timeline nor study design restriction that were published on PubMed. A traditional interplay stands for CLA MTC MEN2 (not MEN3) confirmation. While connection has been reported more than three decades, there is still a large gap understanding addressing it. The majority patients MEN2A-CLA have codon 634; hence, it suggests involvement this specific cysteine residue both disorders (most data agree one-third C634-positive subjects CLA, but ranges are between 9% 50%). Females seem prone MEN2-CLA males. Non-C634 germline included (at low statistical evidence) following: V804M mutation exon 14 MTC-CLA (CLA upper back); S891A 15 binding OSMR variant G513D (familial comprising lower legs thighs, back, shoulders, arms, forearms); C611Y interscapular region), respectively. Typically, detected early age (from childhood until young adulthood) before actual identification unless screening protocols already applied. time frame was 5 60 years according one study. same family not necessarily associated presentation. In MTC/MEN2 subjects, most affected area scapular region back. Alternatively, another hypothesis highlighted fact secondary long-term prurit/notalgia paresthetica (NP) MTC/MEN2. p. may play role modifying evolutionary processes co-harboring mutations (further studies necessary sustain aspect). Awareness CLA-positive essential, including decision testing selected cases.

Language: Английский

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