Recent Advances in Simulation Studies on the Protein Corona DOI Creative Commons
Hwankyu Lee

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(11), P. 1419 - 1419

Published: Nov. 6, 2024

When flowing through the blood stream, drug carriers such as nanoparticles encounter hundreds of plasma proteins, forming a protein layer on nanoparticle surface, known "protein corona". Since corona influences size, shape, and surface properties nanoparticles, it can modulate their circulating lifetime, cytotoxicity, targeting efficiency. Therefore, understanding mechanism formation at atomic scale is crucial, which has become possible due to advances in computer power simulation methodologies. This review covers following topics: (1) structure, dynamics, composition nanoparticles; (2) effects concentration ionic strength formation; (3) particle morphology, (4) interactions among lipids, membranes, with corona. For each topic, mesoscale, coarse-grained, all-atom molecular dynamics simulations since 2020 are discussed. These not only successfully reproduce experimental observations but also provide physical insights into formation. In particular, these findings be applied manipulate that target specific cells, aiding rational design nanomedicines for delivery applications.

Language: Английский

Lipid Nanoparticle (LNP) Delivery Carrier-Assisted Targeted Controlled Release mRNA Vaccines in Tumor Immunity DOI Creative Commons
Liusheng Wu, Xiaoqiang Li,

Xinye Qian

et al.

Vaccines, Journal Year: 2024, Volume and Issue: 12(2), P. 186 - 186

Published: Feb. 12, 2024

In recent years, lipid nanoparticles (LNPs) have attracted extensive attention in tumor immunotherapy. Targeting immune cells cancer therapy has become a strategy of great research interest. mRNA vaccines are potential choice for immunotherapy, due to their ability directly encode antigen proteins and stimulate strong response. However, the mode delivery lack stability key issues limiting its application. LNPs an excellent carrier, structural biocompatibility make them effective means delivering specific targets. This study summarizes progress LNP carrier-assisted targeted controlled release immunity. The role improving stability, immunogenicity, targeting is discussed. review aims systematically summarize latest immunity provide new ideas strategies as well more treatment plans patients.

Language: Английский

Citations

58
Optimized lipid nanoparticles (LNPs) for organ-selective nucleic acids delivery in vivo DOI Creative Commons
Tian Zhang,

Han Yin,

Li Yu

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(6), P. 109804 - 109804

Published: April 23, 2024

Nucleic acid therapeutics offer tremendous promise for addressing a wide range of common public health conditions. However, the

Language: Английский

Citations

14
Effect of Lipid Nanoparticle Physico-Chemical Properties and Composition on Their Interaction with the Immune System DOI Creative Commons
Laura Catenacci,

Rachele Rossi,

Francesca Sechi

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(12), P. 1521 - 1521

Published: Nov. 26, 2024

Lipid nanoparticles (LNPs) have shown promise as a delivery system for nucleic acid-based therapeutics, including DNA, siRNA, and mRNA vaccines. The immune plays critical role in the response to these nanocarriers, with innate cells initiating an early adaptive mediating more specific reaction, sometimes leading potential adverse effects. Recent studies that LNPs is mediated by Toll-like receptors (TLRs) other pattern recognition (PRRs), which recognize lipid components of nanoparticles. This can trigger activation inflammatory pathways production cytokines chemokines, effects such fever, inflammation, pain at injection site. On hand, appears be primarily directed against protein encoded cargo, little evidence ongoing LNP itself. Understanding relationship between development safe effective systems. In fact, targeting essential develop vaccines, well therapies cancer or infections. There lack research literature has systematically studied factors influence interaction further needed better elucidate mechanisms underlying LNPs. this review, we discuss LNPs’ composition, physico-chemical properties, size, shape, surface charge, corona formation affect reactivity system, thus providing guide on new formulations could gain favorable efficacy/safety profile.

Language: Английский

Citations

11
mRNA lipid nanoparticle formulation, characterization and evaluation DOI
Yutian Ma,

Rachel VanKeulen‐Miller,

Owen S. Fenton

et al.

Nature Protocols, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Language: Английский

Citations

1
Sources of biases in the in vitro testing of nanomaterials: the role of the biomolecular corona DOI Creative Commons
Valentina Castagnola, Valeria Tomati, Luca Boselli

et al.

Nanoscale Horizons, Journal Year: 2024, Volume and Issue: 9(5), P. 799 - 816

Published: Jan. 1, 2024

A comparative journey into biomolecular corona features involving proteomics, lipidomics, high throughput in vitro screening, and molecular feature analysis to investigate the vivo / bias for nanomaterials testing biology.

Language: Английский

Citations

4
Looking back, moving forward: protein corona of lipid nanoparticles DOI
Yue Gao,

Yeqi Huang,

Chuanyu Ren

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(23), P. 5573 - 5588

Published: Jan. 1, 2024

Intelligent delivery of lipid nanoparticles can be achieved through rational design protein corona as a “troublemaker”.

Language: Английский

Citations

4
Stimuli-Responsive Nanogel/Microgel Hybrids as Targeted Drug Delivery Systems: A Comprehensive Review DOI

Amrita Ghosh Majumdar,

Biswajit Pany,

Sankha Subhra Parua

et al.

BioNanoScience, Journal Year: 2024, Volume and Issue: 14(3), P. 3496 - 3521

Published: Aug. 13, 2024

Language: Английский

Citations

4
Molecular Dynamics Simulations of Protein Corona Formation on Membrane Surfaces: Effects of Lipid Composition and PEGylation on Selective Plasma Protein Adsorption DOI
Hwankyu Lee

Molecular Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

The adsorption of plasma proteins (human serum albumin (SA) and apolipoproteins A-I E-III) onto various lipid bilayers is simulated. With three different binding orientations for each protein, free energy calculations from umbrella sampling simulations show stronger SA to the bilayer composed lipids with smaller headgroups anionic rather than cationic or zwitterionic lipids, in agreement experiments. Anionic residues form hydrogen bonds more readily amine larger trimethylammonium headgroups, where nitrogen sterically hindered. In contrast, predominantly phosphate groups indicating that protein-bilayer attributed facilitated by electrostatic attraction, depending on electrostatics size headgroups. For grafted polyethylene glycol (PEG), strength decreases while increases, consistent experiments, due bonding hydrophobic interactions between PEG. These findings help explain experimental observations regarding abundance specific adsorbed liposomes suggest manipulating composition PEGylation attract liposome-based drug carriers.

Language: Английский

Citations

0
Albumin Corona Overturns Long‐Acting Behaviors of Myristic Acid‐Conjugated Quetiapine Nanosuspension DOI Creative Commons
Hy Dinh Nguyen, Hai V. Ngo, Beom‐Jin Lee

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: May 19, 2025

Abstract This work aimed to investigate the interaction of a self‐assembled myristic acid‐conjugated quetiapine nanosuspension (QMN) with human serum albumin and its overturning effect on QMN's long‐acting performance. Albumin corona formation modified physicochemical properties pharmacokinetic profile QMN by pH‐responsiveness nano‐aggregation behavior. The adsorption is initially triggered electrostatic forces later hydrophobic‐hydrophobic interactions conformational change structure. While highly susceptible ionic strength, pH, concentration in solution, albumin‐precoated (A‐QMN) stabilized particle size reversed surface charge from ≈+60 −16 mV, annulling pH‐responsive nanoaggregation behaviors under physiological pH conditions. Consequently, A‐QMNs exhibited much faster vitro release more rapid vivo absorption, resulting huge initial burst shorter duration within one week plasma concentration‐time profiles compared extended five‐week following intramuscular injection beagle dogs. These findings indicated important role proteins kinetics pharmacokinetics nanoparticles. manipulation protein can be utilized control properties, biological states, intended nanosuspensions.

Language: Английский

Citations

0
Enhancing Immune Responses against SARS-CoV-2 Variants in Aged Mice with INDUK: A Chimeric DNA Vaccine Encoding the Spike S1-TM Subunits DOI Creative Commons
Lishan Cui, Junbiao Wang, Fiorenza Orlando

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(32), P. 34624 - 34635

Published: July 30, 2024

Currently available vaccines against COVID-19 showed high efficacy the original strain of SARS-CoV-2 but progressively lower new variants. In response to emerging strains, we propose chimeric DNA encoding spike antigen, including a combination selected key mutations from different variants concern. We developed two vaccines, pVAX-S1-TM-D614G and pVAX-S1-TM-INDUK (INDUK), S1 subunit in fusion with transmembrane region that allows protein trimerization as predicted by

Language: Английский

Citations

2