ACS Nano,
Journal Year:
2021,
Volume and Issue:
15(5), P. 7848 - 7859
Published: May 7, 2021
Clustered,
regularly
interspaced
short
palindromic
repeats
(CRISPR)-based
diagnoses,
derived
from
gene-editing
technology,
have
been
exploited
for
less
than
5
years
and
are
now
reaching
the
stage
of
precommercial
use.
CRISPR
tools
some
notable
features,
such
as
recognition
at
physiological
temperature,
excellent
specificity,
high-efficiency
signal
amplification
capabilities.
These
characteristics
promising
development
next-generation
diagnostic
technologies.
In
this
Perspective,
we
present
a
detailed
summary
which
micro/nanotechnologies
play
roles
in
advancement
diagnosis
how
they
involved.
The
use
nanoprobes,
nanochips,
nanodevices,
microfluidic
lateral
flow
strips,
etc.
detection
systems
has
led
to
new
opportunities
CRISPR-based
assay
development,
achieving
equipment-free
detection,
providing
more
compact
systems,
improving
sensitivity
quantitative
Although
tremendous
progress
made,
not
yet
reached
its
full
potential.
We
discuss
upcoming
improvements
will
continue
key
roles.
National Science Review,
Journal Year:
2022,
Volume and Issue:
9(8)
Published: June 3, 2022
The
outbreak
of
the
COVID-19
pandemic
was
partially
due
to
challenge
identifying
asymptomatic
and
presymptomatic
carriers
virus,
thus
highlights
a
strong
motivation
for
diagnostics
with
high
sensitivity
that
can
be
rapidly
deployed.
On
other
hand,
several
concerning
SARS-CoV-2
variants,
including
Omicron,
are
required
identified
as
soon
samples
'positive'.
Unfortunately,
traditional
PCR
test
does
not
allow
their
specific
identification.
Herein,
first
time,
we
have
developed
MOPCS
(Methodologies
Photonic
CRISPR
Sensing),
which
combines
an
optical
sensing
technology-surface
plasmon
resonance
(SPR)
'gene
scissors'
clustered
regularly
interspaced
short
palindromic
repeat
(CRISPR)
technique
achieve
both
specificity
when
it
comes
measurement
viral
variants.
is
low-cost,
CRISPR/Cas12a-system-empowered
SPR
gene-detecting
platform
analyze
RNA,
without
need
amplification,
within
38
min
from
sample
input
results
output,
limit
detection
15
fM.
achieves
highly
sensitive
analysis
SARS-CoV-2,
mutations
appear
in
variants
B.1.617.2
(Delta),
B.1.1.529
(Omicron)
BA.1
(a
subtype
Omicron).
This
also
used
some
recently
collected
patient
local
China,
by
Centers
Disease
Control
Prevention.
innovative
CRISPR-empowered
will
further
contribute
fast,
accurate
target
nucleic
acid
sequences
single-base
mutations.
Chemical Society Reviews,
Journal Year:
2021,
Volume and Issue:
51(1), P. 329 - 375
Published: Dec. 13, 2021
This
review
article
deals
with
the
concepts,
principles
and
applications
of
visible-light
near-infrared
(NIR)
fluorescence
surface-enhanced
Raman
scattering
(SERS)
in
vitro
point-of-care
testing
(POCT)
vivo
bio-imaging.
It
has
discussed
how
to
utilize
biological
transparency
windows
improve
penetration
depth
signal-to-noise
ratio,
use
surface
plasmon
resonance
(SPR)
amplify
SERS
signals.
highlighted
some
plasmonic
probes.
also
reviewed
design
strategies
fluorescent
sensors
detection
metal
ions,
small
molecules,
proteins
nucleic
acids.
Particularly,
it
provided
perspectives
on
integration
into
microfluidic
chips
as
lab-on-chips
realize
testing.
active
devices
non-paper-
or
paper-based
lateral
flow
assays
for
diagnostics.
In
addition,
this
NIR
bio-imaging
platforms
monitoring
physiological
processes
disease
progression
live
cells
tissues.
Moreover,
POCT
toxins,
heavy
metals,
illicit
drugs,
cancers,
traumatic
brain
injuries,
infectious
diseases
such
COVID-19,
influenza,
HIV
sepsis.
ACS Sensors,
Journal Year:
2021,
Volume and Issue:
6(3), P. 1086 - 1093
Published: March 8, 2021
The
outbreak
of
COVID-19
caused
a
worldwide
public
health
crisis.
Large-scale
population
screening
is
an
effective
means
to
control
the
spread
COVID-19.
Reverse
transcription–polymerase
chain
reaction
(RT-qPCR)
and
serology
assays
are
most
available
techniques
for
SARS-CoV-2
detection;
however,
they
suffer
from
either
less
sensitivity
accuracy
or
low
instrument
accessibility
screening.
To
balance
sensitivity,
specificity,
test
availability,
here,
we
developed
enhanced
colorimetry,
which
termed
as
magnetic
pull-down-assisted
colorimetric
method
based
on
CRISPR/Cas12a
system
(M-CDC),
detection.
By
this
method,
RNA
synthetic
sequences
cultured
viruses
can
be
detected
by
naked
eye
gold
nanoparticle
(AuNP)
probes,
with
detection
limit
50
copies
per
reaction.
With
CRISPR/Cas12a-assisted
detection,
specifically
distinguished
other
closely
related
viruses.
M-CDC
was
further
used
analyze
41
clinical
samples,
whose
performance
95.12%,
consistent
that
approved
Clinical
RT-qPCR
Diagnosis
kit.
not
dependent
sophisticated
instruments,
makes
it
potentially
valuable
applied
under
poor
conditions.
Advanced Materials,
Journal Year:
2022,
Volume and Issue:
34(26)
Published: March 8, 2022
Advances
in
wearable
epidermal
sensors
have
revolutionized
the
way
that
physiological
signals
are
captured
and
measured
for
health
monitoring.
One
major
challenge
is
to
convert
easily
readable
a
convenient
way.
possibility
based
on
visible
readouts.
There
range
of
materials
whose
optical
properties
can
be
tuned
by
parameters
such
as
temperature,
pH,
light,
electric
fields.
Herein,
this
review
covers
highlights
set
with
tunable
their
integration
into
Specifically,
recent
progress,
fabrication,
applications
these
summarized
discussed.
Finally,
challenges
perspectives
next
generation
devices
proposed.
ACS Nano,
Journal Year:
2021,
Volume and Issue:
15(8), P. 13475 - 13485
Published: Aug. 9, 2021
Nucleic
acid
biomarkers
have
been
widely
used
to
detect
various
viral-associated
diseases,
including
the
recent
pandemic
COVID-19.
The
CRISPR-Cas-based
trans-activating
phenomenon
has
shown
excellent
potential
for
developing
sensitive
and
selective
detection
of
nucleic
acids.
However,
amplification
steps
are
typically
required
when
monitoring
target
is
needed.
To
overcome
aforementioned
challenges,
we
developed
a
CRISPR-Cas12a-based
amplification-free
biosensor
by
surface-enhanced
Raman
spectroscopy
(SERS)-assisted
ultrasensitive
system.
We
integrated
activated
CRISPR-Cas12a
viral
DNA
with
Raman-sensitive
system
composed
ssDNA-immobilized
probe-functionalized
Au
nanoparticles
(RAuNPs)
on
graphene
oxide
(GO)/triangle
nanoflower
array.
Using
this
CRISPR-based
improved
sensitivity
multiviral
DNAs
such
as
hepatitis
B
virus
(HBV),
human
papillomavirus
16
(HPV-16),
HPV-18
an
extremely
low
limit
vast
range
from
1
aM
100
pM
without
steps.
suggest
that
acids
can
be
applied
precise
early
diagnosis
infections,
cancers,
several
genetic
diseases.
Chemical Communications,
Journal Year:
2021,
Volume and Issue:
57(71), P. 8977 - 8980
Published: Jan. 1, 2021
This
study
reports
a
photoelectrochemical
biosensor
for
dopamine-loaded
liposome-encoded
magnetic
beads
cleaved
by
CRISPR/Cas
12a
system
the
quantification
of
human
papilloma
virus-related
DNA
using
neodymium-doped
BiOBr
nanosheets.
