CRISPR-Cas12a-Derived Photoelectrochemical Biosensor for Point-Of-Care Diagnosis of Nucleic Acid

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(20), P. 7442 - 7448

Published: May 12, 2022

This work presented a point-of-care (POC) photoelectrochemical (PEC) biosensing for the detection of human papillomavirus-16 (HPV-16) on portable electrochemical system by using CRISPR-Cas12a trans-cleaving G-quadruplex biorecognition/amplification and hollow In2O3-In2S3-modified screen-printed electrode (In2O3-In2S3/SPE) as photoactive material. G-quadruplexes were capable biocatalytic precipitation (H2O2-mediated 4-chloro-1-naphthol oxidation) In2O3-In2S3/SPE surface, resulting in weakened photocurrent, but suffered from trans-cleavage when specifically recognized analyte. The photocurrent results could be directly observed with card-sized device via smartphone, which displayed high-value these positive samples, while low-value target-free samples. Such exhibited satisfying responses toward HPV-16 within wide working range 5.0 to 5000 pM allowed at concentration low 1.2 pM. proposed assay provided smartphone signal readout enable rapid screening PEC determination without sophisticated instruments, thus meeting requirements remote areas resource-limited settings. We envision that combining an efficient biometric sensing platform wireless will high-throughput POC diagnostic analysis.

Language: Английский

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Liposome-Mediated In Situ Formation of Type-I Heterojunction for Amplified Photoelectrochemical Immunoassay

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(11), P. 4859 - 4865

Published: March 9, 2022

Exploiting innovative sensing mechanisms and their rational implementation for selective sensitive detection has recently become one of the mainstream research directions photoelectrochemical (PEC) bioanalysis. In contrast to existing conventional strategies, this study presents a new liposome-mediated method via in situ combining ZnInS nanosheets (ZIS NSs) with SnS2 form ZIS NSs/SnS2 type-I heterojunction on fluorine-doped tin oxide (FTO) electrodes highly PEC immunoassays. Specifically, alkaline phosphatase (ALP)-encapsulated liposomes were confined within 96-well plates by sandwich immunorecognition subsequently subjected lysis treatment. Enzymatically produced H2S released ALP was then directed react Sn(IV) engender FTO/ZIS NSs-Sn(IV) electrode, resulting change photogenerated electron–hole transfer path photoelectrode reduction current signaling. Exemplified heart-type fatty acid binding protein (h-FABP) as target, constructed sensor showed good stability selectivity biosensing system. Under optimal conditions, as-prepared platform displayed high sensitivity h-FABP dynamic linear response range 0.1–1000 pg/mL lower limit 55 fg/mL. This immunoassay based establishment, providing protocol analyzing various targets interest.

Language: Английский

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Size-Controlled Engineering Photoelectrochemical Biosensor for Human Papillomavirus-16 Based on CRISPR-Cas12a-Induced Disassembly of Z-Scheme Heterojunctions

ACS Sensors, Journal Year: 2022, Volume and Issue: 7(5), P. 1593 - 1601

Published: May 5, 2022

Photoelectrochemical (PEC) biosensors incorporating biomolecular recognition with photon-to-electron conversion capabilities of the photoactive species have been developed for molecular diagnosis, but most involve difficulty in adjusting band gap positions and are unsuitable PEC biodetection. In this work, an innovative biosensor combined quantum size-controlled engineering based on confinement by controlling size was designed detection human papillomavirus-16 (HPV-16) through CRISPR-Cas12a (Cpf1)-induced disassembly Z-scheme heterojunction. To best our knowledge, that precisely tunes properties materials is first utilized bioanalysis. Based effect, light absorption efficiency charge-transfer rate were tuned to suitable levels obtain performance. After incubation target HPV-16, binding Cas12a-crRNA double-stranded DNA (dsDNA) stimulated activity indiscriminate cleavage toward single-stranded (ssDNA), resulting a decrease photocurrent due blocking electron transfer By optimizing experimental conditions, sensing system exhibited incredible response HPV-16 range from 3.0 pM 600 nM limit 1.0 pM. Impressively, application effect could stimulate more interest precise design structure improve

Language: Английский

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Photoelectrochemical bioanalysis of microRNA on yolk-in-shell Au@CdS based on the catalytic hairpin assembly-mediated CRISPR-Cas12a system

Chemical Communications, Journal Year: 2022, Volume and Issue: 58(54), P. 7562 - 7565

Published: Jan. 1, 2022

This work reports on the proof-of-concept of a photoelectrochemical (PEC) biosensor with horseradish peroxidase-single stranded DNA-encoded magnetic bead (MB-ssDNA-HRP) signal probe cleaved by catalytic hairpin assembly (CHA)-mediated clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12a system for quantification microRNA (miR-21) using yolk-in-shell Au@CdS as photoactive material.

Language: Английский

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CRISPR/Cas12a-based photoelectrochemical sensing of microRNA on reduced graphene oxide-anchored Bi2WO6 coupling with catalytic hairpin assembly

Sensors and Actuators B Chemical, Journal Year: 2022, Volume and Issue: 369, P. 132307 - 132307

Published: July 5, 2022

Language: Английский

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Photoactivatable CRISPR/Cas12a Strategy for One-Pot DETECTR Molecular Diagnosis

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(27), P. 9724 - 9731

Published: June 28, 2022

As a golden partner of recombinase polymerase amplification (RPA), CRISPR/Cas12a has been proven to solve the false-positive problem caused by nonspecific perfectly; meanwhile, its trans-cleave activity further enhanced sensitivity. However, solution transfer operation after tube cap opening greatly increases risk aerosol contamination amplicon, which is inconsistent with point-of-care (POC) diagnostics requirements. This study proposes photoactivated strategy achieve one-pot high-sensitivity nucleic acid detection. Using photocleavable complementary ssDNA block crRNA, RPA can smoothly pass through exponential interval without being affected activated Cas12a in critical early stage. After enough amplicons were produced, test was short bursts ultraviolet radiation at 365 nm. method achieved sensitivity 2.5 copies within 40 min. simple and sensitive effectively avoid amplicon lower threshold for molecular POC.

Language: Английский

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Optically Active Nanomaterials and Its Biosensing Applications—A Review

Biosensors, Journal Year: 2023, Volume and Issue: 13(1), P. 85 - 85

Published: Jan. 4, 2023

This article discusses optically active nanomaterials and their optical biosensing applications. In addition to enhancing sensitivity, these also increase biocompatibility. For this reason, nanomaterials, particularly those based on chemical compositions, such as carbon-based inorganic-based organic-based composite-based for applications are investigated thoroughly. These used extensively in the field of fiber optic improve response time, detection limit, nature specificity. Consequently, describes contemporary application-based research that will be great use researchers nanomaterial-based sensing field. The difficulties encountered during synthesis, characterization, application enumerated, future prospects outlined reader's benefit.

Language: Английский

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The development of a fluorescence/colorimetric biosensor based on the cleavage activity of CRISPR-Cas12a for the detection of non-nucleic acid targets

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 449, P. 131044 - 131044

Published: Feb. 20, 2023

Language: Английский

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Ultrasensitive and Specific Clustered Regularly Interspaced Short Palindromic Repeats Empowered a Plasmonic Fiber Tip System for Amplification-Free Monkeypox Virus Detection and Genotyping

ACS Nano, Journal Year: 2023, Volume and Issue: 17(13), P. 12903 - 12914

Published: June 29, 2023

The urgent necessity for highly sensitive diagnostic tools has been accentuated by the ongoing mpox (monkeypox) virus pandemic due to complexity in identifying asymptomatic and presymptomatic carriers. Traditional polymerase chain reaction-based tests, despite their effectiveness, are hampered limited specificity, expensive bulky equipment, labor-intensive operations, time-consuming procedures. In this study, we present a clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a-based platform with surface plasmon resonance-based fiber tip (CRISPR-SPR-FT) biosensor. compact CRISPR-SPR-FT biosensor, 125 μm diameter, offers high stability portability, enabling exceptional specificity diagnosis precise identification of samples fatal mutation site (L108F)

Language: Английский

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Highly sensitive and reliable detection of microRNA for clinically disease surveillance using SERS biosensor integrated with catalytic hairpin assembly amplification technology

Biosensors and Bioelectronics, Journal Year: 2022, Volume and Issue: 208, P. 114236 - 114236

Published: April 1, 2022

Language: Английский

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