ACS Nano,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 27, 2024
Repairing
cartilage
tissue
is
a
serious
global
challenge.
Herein,
we
focus
on
wood
skeletal
structures
that
are
highly
porous
for
cell
penetration
yet
have
load-bearing
strength,
and
aim
to
synthesize
wood-derived
hydrogels
with
the
ability
regenerate
tissues.
The
were
synthesized
by
delignification
subsequent
intercalation
of
citric
acid
(CA),
which
involved
in
tricarboxylic
cycles
essential
energy
production,
N-acetylglucosamine
(NAG),
glycosaminoglycan,
among
cellulose
microfibrils.
CA
NAG
increased
amorphous
region
microfibrils
endowed
them
flexibility
while
maintaining
structure
wood.
Consequently,
CA-NAG-treated
became
twistable
bendable,
acquired
stiffness,
compressive
water
content,
cushioning
characteristics
similar
those
cartilage.
In
rabbit
femur
defects,
induced
differentiation
surrounding
cells
into
chondrocytes.
repaired
whereas
collagen
scaffolds,
delignified
materials,
CA-treated
did
not.
exhibit
superior
structural
mechanical
over
conventional
cellulose-fiber-containing
scaffolds.
Furthermore,
can
effectively
repair
their
own,
natural
synthetic
polymeric
materials
need
be
combined
growth
factors
achieve
sufficient
therapeutic
effect.
Therefore,
successfully
address
limitations
current
therapies
either
fail
articular
or
sacrifice
healthy
To
our
knowledge,
this
pioneer
study
utilization
thinned
engineering,
will
contribute
solving
both
health
environmental
problems
creating
sustainable
society.
Advanced Healthcare Materials,
Journal Year:
2025,
Volume and Issue:
14(4)
Published: Jan. 5, 2025
Prevalence
of
osteoarthritis
has
been
increasing
in
aging
populations,
which
necessitated
the
use
advanced
biomedical
treatments.
These
involve
grafts
or
delivering
drug
molecules
entrapped
scaffolds.
However,
such
treatments
often
show
suboptimal
therapeutic
effects
due
to
poor
half-life
and
off-target
molecules.
As
a
countermeasure,
3D
printable
robust
hydrogel-based
tissue-repair
platform
is
developed
containing
decellularized
extracellular
matrix
(dECM)
from
differentiated
mammalian
cells
as
cargo.
Here,
pre-osteoblastic
pre-chondrogenic
murine
are
vitro,
decellularized,
incorporated
into
methacrylated
gelatin
(GelMA)
solutions
form
osteogenic
(GelO)
chondrogenic
(GelC)
hydrogels,
respectively.
Integrating
bioactive
dECM
cell
sources
allows
GelO
GelC
induce
differentiation
human
adipose-derived
stem
(hASCs)
toward
lineages.
Further,
can
be
covalently
adhered
using
carbodiimide
coupling
reaction,
forming
multi-layered
hydrogel
with
potential
application
osteochondral
plug.
The
designed
also
hASCs
vitro.
In
conclusion,
carrying
printed
offers
promising
new
cell-free
strategy
for
bone
cartilage
repair
future
management.
ACS Applied Materials & Interfaces,
Journal Year:
2024,
Volume and Issue:
16(6), P. 6772 - 6788
Published: Jan. 31, 2024
Owing
to
dysfunction
of
the
uterus,
millions
couples
around
world
suffer
from
infertility.
Different
conventional
treatments,
tissue
engineering
provides
a
new
and
promising
approach
deal
with
difficult
problems
such
as
human
or
organ
failure.
Adopting
scaffold-based
engineering,
three-dimensional
(3D)
porous
scaffolds
in
combination
stem
cells
appropriate
biomolecules
may
be
constructed
for
uterine
regeneration.
In
this
study,
hierarchical
scaffold,
which
mimicked
structure
functions,
was
designed,
biomimicking
were
then
successfully
fabricated
using
solvent
casting,
layer-by-layer
assembly,
3D
bioprinting
techniques.
For
multilayered,
structured
scaffolds,
poly(l-lactide-co-trimethylene
carbonate)
(PLLA-co-TMC,
"PLATMC"
short)
poly(lactic
acid-co-glycolic
acid)
(PLGA)
blends
first
used
fabricate
shape-morphing
layer
mimic
function
myometrium
tissue.
The
PLATMC/PLGA
polymer
blend
highly
stretchable.
Subsequently,
after
etching
surface
employing
estradiol
(E2),
polydopamine
(PDA),
hyaluronic
acid
(HA),
PDA@E2/HA
multilayer
films
formed
on
build
an
intelligent
delivery
platform
enable
controlled
sustained
release
E2.
also
improved
biological
performance
scaffold.
Finally,
bone
marrow-derived
mesenchymal
cell
(BMSC)-laden
hydrogel
[which
gelatin
methacryloyl
(GelMA)
(Gel)]
printed
thereby
completing
construction
BMSCs
GelMA/Gel
exhibited
excellent
viability
could
spread
released
eventually
upon
biodegradation
hydrogel.
It
shown
that
hierarchically
evolve
initial
flat
shape
into
tubular
completely
aqueous
environment
at
37
°C,
fulfilling
requirement
curved
engineering.
shape,
high
stretchability,
E2
appear
very
Biopolymers,
Journal Year:
2025,
Volume and Issue:
116(1)
Published: Jan. 1, 2025
ABSTRACT
Cartilage
damage
resulting
from
trauma
demonstrates
a
poor
capacity
for
repair
due
to
its
avascular
nature.
tissue
engineering
offers
unique
therapeutic
option
cartilage
recovery.
In
this
study,
methylcellulose
(MC)/gelatin
(GEL)
hydrogels
(MC10G20,
MC12.5G20,
MC15G20,
and
MC17.5G20)
were
developed
assess
compare
their
chemical,
mechanical,
biological
characteristics
repair.
First,
the
interaction
between
MC
GEL
after
blending
subsequent
crosslinking
with
EDC/NHS
was
confirmed
by
using
FTIR.
Mechanical
tests
under
compression
test
revealed
that
hydrogels'
resistance
both
elastic
plastic
deformation
increased
higher
wt.%
of
MC.
The
%
strain
doubled
addition
MC,
likely
abundant
hydrogen
bonding
polymeric
chains.
Furthermore,
compressive
modulus
MC/GEL
approximately
0.2
MPa,
closely
matching
human
tissue.
Similarly,
water
retention
over
7
days
as
content
increased.
Additionally,
SEM
images
showed
incorporation
introduced
porosity
diameters
ranging
10
50
μm,
similar
size
pores
in
native
cartilage.
vitro
cell
culture
studies
biocompatibility
hydrogels.
Fluorescence
staining
2.5‐fold
increase
F‐actin
following
into
Overall,
study
highlights
potential
engineering,
however,
further
research
is
required
full
potential.
Bratislavské lekárske listy/Bratislava medical journal,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 27, 2025
Abstract
3D
printing
technologies
are
manufacturing
based
on
computer-designed
digital
models
that
allow
fabrication
of
layered
three-dimensional
objects.
This
review
aims
to
present
a
summary
the
literature
published
3D-printed
polymer
and
composite
materials
in
dentistry.
A
search
was
performed
using
PubMed
database
identify
eligible
articles.
In
total
508
articles
were
identified
original
query,
with
362
being
eliminated
exclusion
criteria
146
screened
their
abstracts,
68
studied
detail.
Subsequently,
these
divided
into
three
groups
area
application:
(1)
restorative
dentistry,
which
included
printed
crowns,
bridges,
veneers;
(2)
regenerative
dentistry
tissue
engineering,
such
as
scaffolds;
(3)
oral
guides
other
appliances,
surgical
guides,
dental
implants,
splints.
this
technology
is
described,
including
its
benefits
regarding
working
time,
accuracy
overall
design
products.
The
shows
most
application
printable
polymers
composites
Even
though
for
properties
effects
human
body
well
environment,
novel
specific
revolutionary
characteristics
have
emerged
recent
years
given
special
attention.
However,
more
research
needed
ensure
safety
use
confirm
both
vivo
vitro
conditions.
Biomedical Physics & Engineering Express,
Journal Year:
2023,
Volume and Issue:
9(6), P. 065014 - 065014
Published: Aug. 31, 2023
3D
printing
is
an
effective
method
to
prepare
scaffolds
for
tissue
engineering
applications.
However,
optimization
of
conditions
obtain
suitable
mechanical
properties
various
applications
costly
and
time
consuming.
To
address
this
problem,
in
study,
scikit-learn
Python
machine
learning
library
was
used
apply
four
learning-based
approaches
which
are
ordinary
least
squares
(OLS)
linear
regression,
random
forest
(RF),
light
gradient
Boost
(LGBM),
extreme
boosting
(XGB)
artificial
neural
network
models
understand
the
relationship
between
parameters
tensile
strength
poly(lactic
acid)
(PLA).
68
combinations
process
nozzle
temperature,
speed,
layer
height
were
from
investigated
research
papers.
Then,
datasets
divided
as
training
(80%)
test
(20%).
After
building
OLS
RF,
LGBM,
XGB
models,
correlation
heatmap
feature
importance
each
parameter
values
determined,
respectively.
predicted
real
evaluate
performance
models.
The
results
demonstrate
that
model
most
successful
predicting
among
studied
with
anR2value
0.98
0.94
train
values,
A
closeR2value
also
indicated
there
no
overfitting
data
model.
Finally,
SHAP
analysis
shows
significance
on
prediction
strength.
This
study
can
be
extended
independent
variables
including
pressure,
strut
size
molecular
weight
PLA
dependent
such
elongation
elastic
modulus
may
a
powerful
tool
predict
Biomedical Materials,
Journal Year:
2024,
Volume and Issue:
19(3), P. 035020 - 035020
Published: March 18, 2024
Polycaprolactone
(PCL)
is
a
suitable
material
for
bone
repair
due
to
good
biocompatibility
and
mechanical
properties.
However,
low
bioactivity
hydrophobicity
pose
major
challenges
its
biomedical
applications.
To
overcome
these
limitations,
PCL-based
scaffolds
loaded
with
bioactive
agents
have
been
developed.
Salicin
(Sal)
an
anti-inflammatory
analgesic
herbal
glycoside
osteogenic
potential.
In
the
present
study,
we
aimed
produce
Sal-laden
PCL
(PCL-Sal)
scaffold
healing
Three-dimensional
were
produced
their
biocompatibility,
physical-chemical
characteristics
determined.
The
potential
of
PCL-Sal
was
evaluated
using
marrow
mesenchymal
stem
cells
(BMSCs).
Scaffolds
implanted
into
5
mm
defect
created
in
femur
adult
rats,
new
fraction
determined
micro-computed
tomography
scanning
at
one-month
follow-up.
had
structure,
porosity,
fiber
diameter
construction.
It
also
possessed
higher
rate
hydrophilicity
compared
PCL,
providing
surface
proliferation
differentiation
BMSCs.
Furthermore,
showed
capacity
scavenge
free
radicals
PCL.
improved
confirmed
according
ACS Omega,
Journal Year:
2024,
Volume and Issue:
9(28), P. 30097 - 30108
Published: July 2, 2024
Osteochondral
defects
affect
articular
cartilage,
calcified
and
subchondral
bone.
The
main
problem
that
they
cause
is
a
different
behavior
of
cell
tissue
in
the
osteochondral
bone
part.
Articular
cartilage
composed
mainly
collagen
II,
glycosaminoglycan
(GAG),
water,
has
low
healing
ability
due
to
lack
vascularization.
However,
I,
proteoglycans,
inorganic
composites
such
as
hydroxyapatite.
Due
discrepancy
between
characters
these
two
parts,
it
difficult
find
materials
will
meet
all
structural
other
requirements
for
effective
regeneration.
When
designing
scaffold
an
defect,
variety
are
available,
e.g.,
polymers
(synthetic
natural),
particles,
extracellular
matrix
(ECM)
components.
All
them
require
accurate
characterization
prepared
number
vitro
vivo
tests
before
applied
patients.
Taken
concert,
final
material
needs
mimic
structural,
morphological,
chemical,
cellular
demands
native
tissue.
In
this
review,
we
present
overview
structure
composition
part,
especially
synthetic
with
additives
appropriate
defects.
Finally,
summarize
methods
suitable
evaluating
restoring
