Bioprocess and Biosystems Engineering, Journal Year: 2025, Volume and Issue: unknown
Published: May 20, 2025
Language: Английский
International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 306, P. 141779 - 141779
Published: March 5, 2025
Language: Английский
Citations
1
ACS Biomaterials Science & Engineering, Journal Year: 2024, Volume and Issue: 10(8), P. 5122 - 5135
Published: July 22, 2024
3D extrusion printing has been widely investigated for low-volume production of complex-shaped scaffolds tissue regeneration. Gelatin methacryloyl (GelMA) is used as a baseline material the synthesis biomaterial inks, often with organic/inorganic fillers, to obtain balance between good printability and biophysical properties. The present study demonstrates how 45S5 bioactive glass (BG) addition GelMA concentrations can be tailored develop composite buildability. experimental results suggest that BG consistently decreases compression stiffness, irrespective concentration, albeit within 20% scaffold (without BG). optimal 2 wt % in 7.5 was demonstrated provide best combination buildability route. degradation swelling kinetics increases addition, concentration. Importantly, dissolution simulated body fluid over 3 weeks clearly promoted nucleation growth crystalline calcium phosphate particles, indicating potential GelMA-45S5 promote biomineralization. cytocompatibility assessment using human osteoblasts could demonstrate uncompromised cell proliferation or osteogenic marker expression 21 days culture printable -2 when compared GelMA. thus encourage further investigations GelMA/45S5 system bone engineering applications.
Language: Английский
Citations
6
Bioprinting, Journal Year: 2024, Volume and Issue: unknown, P. e00365 - e00365
Published: Oct. 1, 2024
Language: Английский
Citations
4
Journal of Biomaterials Applications, Journal Year: 2025, Volume and Issue: unknown
Published: April 3, 2025
The last two decaes have witnessed significant efforts to develop gelatin/alginate based scaffolds using variants of 3D printing techniques. However, their biocompatibility for regenerating complex soft tissues remains insufficiently explored. Addressing this gap, we fabricated 3D-printed alginate-gelatin (3A5G) and nanocellulose-reinforced (3A5G1C) hydrogel with clinically relevant dimensions (15 mm diameter, 5 height) the host tissue responses were critically analyzed. distinct advantages nanocellulose in modulating mechanical strength, viscoelasticity, swelling, degradation characteristics established our prior studies. This investigation aimed comprehensively evaluate foreign body response these a rat model. animals exhibited healthy metabolic activity, evidenced by progressive weight gain, localized healing, normal mobility over 30 days. Histological analyses could not reveal any adverse immune reaction at 7- or 30-days, post-implantation. Hematological serum biochemical assessments indicated progression from acute (7 days) sub-acute (30 inflammation, following subcutaneous implantation, without signature systemic toxicity. Immune marker evaluation (TNF-α, CD-8, CD-68, COX-2, IL-6) confirmed absence pathological responses, even incorporation. Immunohistochemical analysis CD31 staining demonstrated enhanced vascularization both 7 toxicity scaffold products favorable outcomes underline potential regeneration. incorporation further scaffolds' functional performance, particularly promoting vascularization, positioning them as promising candidates engineering applications.
Language: Английский
Citations
0
Regenerative Engineering and Translational Medicine, Journal Year: 2025, Volume and Issue: unknown
Published: April 22, 2025
Language: Английский
Citations
0
International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143535 - 143535
Published: April 1, 2025
Language: Английский
Citations
0
ACS Omega, Journal Year: 2025, Volume and Issue: unknown
Published: May 2, 2025
In developing hydrogel scaffolds for the soft tissue regeneration, a number of inorganic or carbonaceous fillers are embedded in alginate/gelatin-based while manufacturing shape fidelity compliant constructs using three-dimensional (3D) extrusion printing. Among spectrum nanofillers, nanohydroxyapatite (nHAP), due to its intrinsic bioactivity, could promote mineralization and interaction with host tissues conferring superior mechanical properties (strength elastic modulus). Against this backdrop, study demonstrates effectiveness nHAP reinforcement tuning several clinically relevant such as rheological properties, swelling, degradation, antimicrobial properties. At higher concentrations (0.75%) matrix (3A5G0.75H), 3.13-fold increment compressive strength was observed, gel stability window thermal cross-linked graft being extended greater than 40 143 °C, respectively. This demonstrated printability nHAP-reinforced ink by fabricating matrix-shaped dimension 20 mm diameter 10 thickness, buildability established making bulk-sized construct up 62 layers (20 height) well-maintained pore interconnectivity, micro-CT analysis. Interestingly, CFU revealed 2.9- 1.5-fold improvement reduction bacterial adhesion 3A5G0.75H respect Escherichia coli Staphylococcus aureus bacteria. Cell culture studies on 3D printed w.r.to NIH 3T3 fibroblast cell line consistent increase viability pronounced filopodial extensions, confirming cytocompatibility their ability support cellular growth during an vitro culture. Taken together, present uncovers process science-based understanding biophysical different inks
Language: Английский
Citations
0
Bioprocess and Biosystems Engineering, Journal Year: 2025, Volume and Issue: unknown
Published: May 20, 2025
Language: Английский
Citations
0