Near-Infrared Probes Designed with Hemicyanine Fluorophores Featuring Rhodamine and 1,8-Naphthalic Derivatives for Viscosity and HSA Detection in Live Cells

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

This paper presents the development of near-infrared (NIR) fluorescent probes,

Language: Английский

A Rhodamine-Based Ratiometric Fluorescent Sensor for Dual-Channel Visible and Near-Infrared Emission Detection of NAD(P)H in Living Cells and Fruit Fly Larvae

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

The detection and dynamic monitoring of intracellular NAD(P)H concentrations are crucial for comprehending cellular metabolism, redox biology, their roles in various physiological pathological processes. To address this need, we introduce sensor A, a near-infrared ratiometric fluorescent real-time, quantitative imaging fluctuations live cells. Sensor A combines 3-quinolinium electron-deficient acceptor with rhodamine dye, offering high sensitivity specificity superior photophysical properties. In its unbound state, emits strongly at 650 nm weakly 465 upon 400 excitation. Upon binding to NAD(P)H, it shows fluorescence increase decrease nm, enabling accurate measurements. also exhibits upconversion when excited 800 or 810 additional flexibility different experimental setups. sensor's response relies on the reduction by forming 1,4-dihydroquinoline donor that enhances quenches emission through photoinduced electron transfer. This mechanism ensures reliable quantification levels while minimizing interference from concentration, excitation intensity, environmental factors. was validated HeLa MD-MB453 cells under metabolic pharmacological conditions, including glucose maltose stimulation treatments chemotherapeutic agents. Co-localization mitochondrial-specific dyes confirmed mitochondrial targeting, precise tracking fluctuations. vivo Drosophila larvae nutrient starvation exposure revealed dose-dependent responses, highlighting potential changes organisms. represents significant advancement imaging, providing powerful tool exploring metabolism biology biomedical research.

Language: Английский

A Quinolinium-Functionalized Hemicyanine Dye for Ratiometric NAD(P)H Sensing in Live Cells, Kidney Tissues, and Drosophila melanogaster

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 138043 - 138043

Published: May 1, 2025

Language: Английский