Cell Reports Methods,
Journal Year:
2022,
Volume and Issue:
2(11), P. 100325 - 100325
Published: Oct. 31, 2022
Single-cell
RNA
sequencing
(scRNA-seq)
and
spatially
resolved
transcriptomics
(SRT)
have
experienced
rapid
development
in
recent
years.
The
findings
of
spaceflight-based
scRNA-seq
SRT
investigations
are
likely
to
improve
our
understanding
life
space
comprehension
gene
expression
various
cell
systems
tissue
dynamics.
However,
compared
their
Earth-based
counterparts,
experiments
conducted
spaceflight
not
the
same
pace
development.
Out
hundreds
datasets
available,
only
a
few
used
SRT.
In
this
perspective
piece,
we
explore
growing
importance
biology
discuss
challenges
considerations
relevant
robust
experimental
design
enable
growth
these
methods
field.
Gels,
Journal Year:
2023,
Volume and Issue:
9(3), P. 185 - 185
Published: Feb. 27, 2023
The
repair
of
skin
injury
has
always
been
a
concern
in
the
medical
field.
As
kind
biopolymer
material
with
special
network
structure
and
function,
collagen-based
hydrogel
widely
used
field
repair.
In
this
paper,
current
research
application
status
primal
hydrogels
recent
years
are
comprehensively
reviewed.
Starting
from
properties
collagen,
preparation,
structural
properties,
emphatically
described.
Meanwhile,
influences
collagen
types,
preparation
methods,
crosslinking
methods
on
discussed.
future
development
prospected,
which
is
expected
to
provide
reference
for
future.
Advanced Healthcare Materials,
Journal Year:
2023,
Volume and Issue:
12(24)
Published: May 8, 2023
Cardiac
safety
assessments
are
significant
in
drug
discovery,
as
drug-induced
cardiotoxicity
(DIC)
is
the
primary
cause
of
attrition.
Despite
heart-on-a-chip
(HoC)
technology
becoming
an
increasingly
popular
tool
for
evaluating
DIC,
its
development
remains
a
challenge
owing
to
anisotropic
cardiac
structure
native
myocardium.
Herein,
multiscale
scaffold
presented
via
hybrid
biofabrication
method
by
combining
3D
printing
with
electrospinning
technology,
where
3D-printed
micrometer-scale
frames
enable
mimicking
interwoven
myocardium
anatomical
and
branched-aligned
electrospun
nanofibers
network
able
directionally
guide
cellular
arrangements.
The
vitro
bioengineered
tissues
then
fabricated
encapsulating
three-layer
scaffolds
within
photocurable
methacrylated
gelatin
hydrogel
shell.
It
demonstrated
that
such
could
contribute
enhancing
cardiomyocyte
maturation
synchronous
beating
behavior.
More
attractively,
integration
self-designed
microfluidic
perfusion
system,
HoC
platform
established
DIC
cardioprotective
efficacy.
Collectively,
these
results
indicate
model
developed
integrating
effectively
recapitulate
clinical
manifestations,
thereby
highlighting
their
efficacy
valuable
preclinical
testing
cardiotoxicity.
Engineering,
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 1, 2024
Organ
damage
or
failure
arising
from
injury,
disease,
and
aging
poses
challenges
due
to
the
body's
limited
regenerative
capabilities.
transplantation
presents
issues
of
donor
shortages
immune
rejection
risks,
necessitating
innovative
solutions.
The
3D
bioprinting
organs
on
demand
offers
promise
in
tissue
engineering
medicine.
In
this
review,
we
explore
state-of-the-art
technologies,
with
a
focus
bioink
cell
type
selections.
We
follow
discussions
advances
solid
organs,
such
as
heart,
liver,
kidney,
pancreas,
highlighting
importance
vascularization
integration.
Finally,
provide
insights
into
key
future
directions
context
clinical
translation
bioprinted
their
large-scale
production.
ACS Applied Bio Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: April 10, 2024
Traditional
tissue
engineering
methods
face
challenges,
such
as
fabrication,
implantation
of
irregularly
shaped
scaffolds,
and
limited
accessibility
for
immediate
healthcare
providers.
In
situ
bioprinting,
an
alternate
strategy,
involves
direct
deposition
biomaterials,
cells,
bioactive
factors
at
the
site,
facilitating
on-site
fabrication
intricate
tissue,
which
can
offer
a
patient-specific
personalized
approach
align
with
principles
precision
medicine.
It
be
applied
using
handled
device
robotic
arms
to
various
tissues,
including
skin,
bone,
cartilage,
muscle,
composite
tissues.
Bioinks,
critical
components
bioprinting
that
support
cell
viability
development,
play
crucial
role
in
success
bioprinting.
This
review
discusses
techniques,
materials
used
bioinks,
their
properties
successful
applications.
Finally,
we
discuss
challenges
future
trends
accelerating
printing
translate
this
technology
clinical
settings
regenerative
Advanced Healthcare Materials,
Journal Year:
2023,
Volume and Issue:
13(1)
Published: July 20, 2023
Cardiovascular
disease
is
a
major
cause
of
mortality
worldwide,
and
current
preclinical
models
including
traditional
animal
2D
cell
culture
have
limitations
in
replicating
human
native
heart
physiology
response
to
drugs.
Heart-on-a-chip
(HoC)
technology
offers
promising
solution
by
combining
the
advantages
cardiac
tissue
engineering
microfluidics
create
vitro
3D
models,
which
can
mimic
key
aspects
microphysiological
systems
provide
controllable
microenvironments.
Herein,
recent
advances
HoC
technologies
are
introduced,
engineered
microtissue
construction
vitro,
microfluidic
chip
fabrication,
microenvironmental
stimulation,
real-time
feedback
systems.
The
development
methods
focused
for
preparation,
advanced
strategies
applications
these
platforms.
Major
challenges
fabrication
discussed
perspective
on
potential
platforms
provided
advance
research
clinical
applications.
