Aqueous Synthesis of Poly(ethylene glycol)‐Amide‐Norbornene‐Carboxylate for Modular Hydrogel Crosslinking

Advanced Materials Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 5, 2025

Abstract Poly(ethylene glycol)‐norbornene (e.g., PEGNB) is a versatile macromer amenable to step‐growth thiol‐norbornene photopolymerization and inverse electron demand Diels–Alder (iEDDA) click reaction. The translational potentials of PEGNB‐based hydrogels have been realized in the areas stem cell differentiation, vitro disease modeling, implantable therapeutic devices, controlled release therapeutics. Even with these advances, prior methods for synthesizing PEGNB all required heavy use organic solvents that pose significant environmental personal health burdens. Here, an all‐aqueous synthesis PEG‐amide‐norbornene‐carboxylate (PEGaNB CA ) reported via reacting carbic anhydride (CA) multi‐arm amino‐terminated PEG. Like previously ester‐bearing counterparts (i.e., PEGeNB ), PEGaNB readily crosslinked into modular by either or tetrazine‐norbornene iEDDA Unlike its counterparts, provided long‐term hydrolytic stability. However, through blending hydrolytically labile , can be engineered undergo tunable degradation. versatility further demonstrated high‐fidelity digital light processing printing situ encapsulation maintenance human induced pluripotent cells (hiPSCs).

Language: Английский

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Trends in Photopolymerizable Bioinks for 3D Bioprinting of Tumor Models

JACS Au, Journal Year: 2023, Volume and Issue: 3(8), P. 2086 - 2106

Published: Aug. 11, 2023

Three-dimensional (3D) bioprinting technologies involving photopolymerizable bioinks (PBs) have attracted enormous attention in recent times owing to their ability recreate complex structures with high resolution, mechanical stability, and favorable printing conditions that are suited for encapsulating cells. 3D bioprinted tissue constructs PBs can offer better insights into the tumor microenvironment platforms drug screening advance cancer research. These enable incorporation of physiologically relevant cell densities, tissue-mimetic stiffness, vascularized channels biochemical gradients models, unlike conventional two-dimensional (2D) cultures or other scaffold fabrication technologies. In this perspective, we present emerging techniques using context research, a specific focus on efforts recapitulate complexity microenvironment. We describe approaches various PB formulations compatible these along attempts bioprint models studying migration metastasis, cell–cell interactions, cell–extracellular matrix cancer. discuss limitations identify unexplored opportunities field clinical commercial translation

Language: Английский

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Photo‐Controllable Smart Hydrogels for Biomedical Application: A Review

Small Methods, Journal Year: 2023, Volume and Issue: 8(1)

Published: Oct. 26, 2023

Abstract Nowadays, smart hydrogels are being widely studied by researchers because of their advantages such as simple preparation, stable performance, response to external stimuli, and easy control behavior. Photo‐controllable (PCHs) a class responsive whose physical chemical properties can be changed when stimulated light at specific wavelengths. Since the source is safe, clean, operate, control, PCHs have broad application prospects in biomedical field. Therefore, this review timely summarizes latest progress field, with an emphasis on design principles typical multiple applications tissue regeneration, tumor therapy, antibacterial diseases diagnosis monitoring, etc. Meanwhile, challenges perspectives widespread practical implementation presented applications. This study hopes that will flourish field provide useful information for interested researchers.

Language: Английский

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Bone-targeted lipoplex-loaded three-dimensional bioprinting bilayer scaffold enhanced bone regeneration

Regenerative Biomaterials, Journal Year: 2024, Volume and Issue: 11

Published: Jan. 1, 2024

Abstract Clinical bone-morphogenetic protein 2 (BMP2) treatment for bone regeneration, often resulting in complications like soft tissue inflammation and ectopic ossification due to high dosages non-specific delivery systems, necessitates research into improved biomaterials better BMP2 stability retention. To tackle this challenge, we introduced a groundbreaking bone-targeted, lipoplex-loaded, three-dimensional bioprinted bilayer scaffold, termed the polycaprolactone-bioink-nanoparticle (PBN) aimed at boosting regeneration. We encapsulated within fibroin nanoparticle based lipoplex (Fibroplex) functionalized it with DSS6 tissue-specific targeting. 3D printing technology enables customized, porous PCL scaffolds healing growth, two-step bioprinting process creating cellular lattice structure bioink grid using gelatin-alginate hydrogel DSS6-Fibroplex, shown support effective nutrient exchange cell growth specific pore sizes. The PBN scaffold is predicted through silico analysis exhibit biased release between tissue, finding validated by vitro osteogenic differentiation assays. was evaluated critical calvarial defects, focusing on sustained delivery, prevention of infiltration controlled fiber membrane size vivo. demonstrated more than eight times longer time that collagen sponge, promoting regeneration defect animal. Our findings suggest enhanced local concentration defects spatial arrangement formation, thereby reducing risk heterotopic ossification.

Language: Английский

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A 3D-printed blood-brain barrier model with tunable topology and cell-matrix interactions

Biofabrication, Journal Year: 2023, Volume and Issue: 16(1), P. 015005 - 015005

Published: Oct. 11, 2023

Abstract Recent developments in digital light processing (DLP) can advance the structural and biochemical complexity of perfusable vitro models blood–brain barrier. Here, we describe a strategy to functionalize complex, DLP-printed vascular with multiple peptide motifs single hydrogel. Different peptides be clicked into walls distinct topologies, or lining channel differ from those bulk The flexibility this approach is used both characterize effects various bioactive domains on endothelial coverage tight junction formation, addition facilitating astrocyte attachment hydrogel surrounding endothelialized vessel mimic endothelial–astrocyte interaction. Peptides derived proteins mediating cell-extracellular matrix (e.g. RGD IKVAV) cell–cell HAVDI) adhesions are mediate cell coverage. HAVDI IKVAV-lined channels exhibit significantly greater endothelialization increased zonula-occluden-1 (ZO-1) localization junctions cells, indicative formation. then create an co-culture model barrier that overcomes limitations previous platforms incapable complex topology tunable domains. This yields adjustable, biofabricated platform interrogate cell-matrix interaction mechanobiology.

Language: Английский

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