MIML: Multiplex Image Machine Learning for High Precision Cell Classification via Mechanical Traits within Microfluidic Systems DOI Creative Commons
Khayrul Islam, Ratul Paul, Shen Wang

et al.

arXiv (Cornell University), Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 1, 2023

Label-free cell classification is advantageous for supplying pristine cells further use or examination, yet existing techniques frequently fall short in terms of specificity and speed. In this study, we address these limitations through the development a novel machine learning framework, Multiplex Image Machine Learning (MIML). This architecture uniquely combines label-free images with biomechanical property data, harnessing vast, often underutilized morphological information intrinsic to each cell. By integrating both types our model offers more holistic understanding cellular properties, utilizing typically discarded traditional models. approach has led remarkable 98.3\% accuracy classification, substantial improvement over models that only consider single data type. MIML been proven effective classifying white blood tumor cells, potential broader application due its inherent flexibility transfer capability. It's particularly similar morphology but distinct properties. innovative significant implications across various fields, from advancing disease diagnostics behavior.

Language: Английский

Vascularized organoid-on-a-chip: design, imaging, and analysis DOI
Tingting Yu,

Qihang Yang,

Bo Peng

et al.

Angiogenesis, Journal Year: 2024, Volume and Issue: 27(2), P. 147 - 172

Published: Feb. 26, 2024

Language: Английский

Citations

8
Formation of various cell-aggregated structures in the core of hydrogel filament using a microfluidic device and its application as an in vitro neuromuscular junction model DOI
Juyeon Kim, GeunHyung Kim

Chemical Engineering Journal, Journal Year: 2023, Volume and Issue: 472, P. 144979 - 144979

Published: July 23, 2023

Language: Английский

Citations

14
Droplet Microfluidics for Current Cancer Research: From Single-Cell Analysis to 3D Cell Culture DOI
Lin Jiang,

Kefan Guo,

Chen Yao

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2024, Volume and Issue: 10(3), P. 1335 - 1354

Published: Feb. 29, 2024

Cancer is the second leading cause of death worldwide. Differences in drug resistance and treatment response caused by heterogeneity cancer cells are primary reasons for poor therapy outcomes patients. In addition, current vitro anticancer drug-screening methods rely on two-dimensional monolayer-cultured cells, which cannot accurately predict behavior vivo. Therefore, a powerful tool to study produce effective tumor models warranted leverage research. Droplet microfluidics has become platform single-cell analysis three-dimensional cell culture spheroids. this review, we discuss use droplet microfluidic technologies, including single- or double-emulsion generation passive- active-droplet manipulation, concisely discussed. Recent advances circulating scaffold-free/based 3D spheroids have been systematically introduced. Finally, challenges that must be overcome further application research

Language: Английский

Citations

4
Microphysiologically Engineered Vessel-Tumor Model to Investigate Vascular Transport Dynamics of Immune Cells DOI Creative Commons
Yuwen Zhao, Yue Wu, Khayrul Islam

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: unknown

Published: April 23, 2024

Cancer immunotherapy has emerged as a promising therapeutic strategy to combat cancer effectively. However, it is hard observe and quantify how this in vivo process happens. Three-dimensional (3D) microfluidic vessel-tumor models offer valuable capability study immune cells transport during progression. We presented an advanced 3D vessel-supported tumor model consisting of the endothelial lumen vessel network for T cells' transportation. The cell through interaction with spheroids was represented monitored vitro. Specifically, we demonstrate that glycocalyx serving can influence endothelium-immune interaction. Furthermore, after vascular transport, programmed death protein 1 (PD-1) checkpoint inhibition influences delivered activated-T on killing evaluated. Our vitro provides microphysiologically engineered platform represent transportation immunotherapy. reported innovative believed have potential explore tumor-induced response mechanism preclinically evaluate immunotherapy's effectiveness.

Language: Английский

Citations

4
GelMA hydrogel: A game-changer in 3D tumor modeling DOI Creative Commons

Weijie Jiao,

Jingxin Shan,

Xue Gong

et al.

Materials Today Chemistry, Journal Year: 2024, Volume and Issue: 38, P. 102111 - 102111

Published: May 16, 2024

Currently, researchers have increasingly focused on 3D in vitro tumor models as they possess the capacity to replicate tissue-like characteristics and cell-cell interactions within microenvironment. Hydrogel, a hydrophilic polymer network that crosslinks structure, has emerged prominent cynosure due its ability absorb water, swell, provide attachment sites for cells. By mimicking extracellular matrix, hydrogel facilitates investigation of cell growth interaction. The gelatin methacrylate (GelMA) garnered significant interest scientific community be mechanically adjusted exceptional biocompatibility. This review primarily focuses recent advancements construction methodology utilizing GelMA hydrogel, including preparation procedures, attributes, performance enhancements. Furthermore, we offer insights into prospective directions advancement hydrogels. It is anticipated hydrogels will find extensive application near future precise fabrication with diverse geometries. These are poised become preferred option investigating microenvironment, tumorigenesis, metastasis, related mechanisms.

Language: Английский

Citations

4
Development of droplet-based microfluidics in generation, manipulation and biomedical application DOI

Yi Cai,

Run‐Ze Sun, Yukun Ren

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113101 - 113101

Published: Feb. 1, 2025

Language: Английский

Citations

0
MIML: multiplex image machine learning for high precision cell classification via mechanical traits within microfluidic systems DOI Creative Commons
Khayrul Islam, Ratul Paul, Shen Wang

et al.

Microsystems & Nanoengineering, Journal Year: 2025, Volume and Issue: 11(1)

Published: March 7, 2025

Label-free cell classification is advantageous for supplying pristine cells further use or examination, yet existing techniques frequently fall short in terms of specificity and speed. In this study, we address these limitations through the development a novel machine learning framework, Multiplex Image Machine Learning (MIML). This architecture uniquely combines label-free images with biomechanical property data, harnessing vast, often underutilized biophysical information intrinsic to each cell. By integrating both types our model offers holistic understanding cellular properties, utilizing typically discarded traditional models. approach has led remarkable 98.3% accuracy classification, substantial improvement over models that rely solely on image data. MIML been proven effective classifying white blood tumor cells, potential broader application due its inherent flexibility transfer capability. It particularly similar morphology but distinct properties. innovative significant implications across various fields, from advancing disease diagnostics behavior.

Language: Английский

Citations

0
Ultrasonic Manipulation in Tissue Engineering DOI Creative Commons

Zijun Guan,

Jianbo Huang, Yang Gao

et al.

Engineered Regeneration, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Acoustofluidic Engineering of Functional Vessel-on-a-Chip DOI Creative Commons
Yue Wu, Yuwen Zhao, Khayrul Islam

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2023, Volume and Issue: 9(11), P. 6273 - 6281

Published: Oct. 3, 2023

Construction of in vitro vascular models is great significance to various biomedical research, such as pharmacokinetics and hemodynamics, thus an important direction tissue engineering. In this work, a standing surface acoustic wave field was constructed spatially arrange suspended endothelial cells into designated patterning. The cell patterning maintained after the withdrawn by solidified hydrogel. Then, interstitial flow provided activate vessel tube formation. Thus, functional vessel-on-a-chip engineered with specific geometry. Vascular function, including perfusability barrier characterized beads loading dextran diffusion, respectively. A computational atomistic simulation model proposed illustrate how solutes cross lipid bilayer. reported acoustofluidic methodology capable facile reproducible fabrication network It promising facilitate development both fundamental research regenerative therapy.

Language: Английский

Citations

8
An Overview of Advancements and Technologies in Vascularization Strategies for Tumor‐On‐A‐Chip Models DOI

Pratibha Parihar,

Naina Sunildutt,

Chethikkattuveli Salih Abdul Rahim

et al.

Advanced Therapeutics, Journal Year: 2024, Volume and Issue: 7(6)

Published: Feb. 22, 2024

Abstract Vascularized tumor on a chip (VToC) entail emulating intricate microvascular networks like those observed in tumors through microfluidic devices, which are meticulously designed to offer faithful representation of cancer vitro, exploration biology, evaluation drug efficacy, and anticipation patient responses therapies. Compared conventional ones, VToC systems hold advantages by creating milieu where physiological conditions for investigating tumor–host interactions pivotal advancement/therapy resilience. Nevertheless, models confront limitations encompassing vascular network replication, biological fidelity, mechanical/chemical integrity, intricacies architectural design. Thus, drawbacks inherent prevailing models’ intricacies, attributes, establishment imperative. This systematic review focuses the recent advancements, technologies explored incorporating models, vascularization approaches investigation, factors/parameters affecting complex microenvironments along with futuristic approach design strategies, fabrication techniques, understanding network, also spheroid. A comprehensive analysis based their practical highlights promising strategies possible applications. will be essential regarding complete overview future direction toward developing efficient compared state‐of‐the‐art VToC.

Language: Английский

Citations

3