Recent Treatment Strategies and Molecular Pathways in Resistance Mechanisms of Antiangiogenic Therapies in Glioblastoma

Cancers, Journal Year: 2024, Volume and Issue: 16(17), P. 2975 - 2975

Published: Aug. 27, 2024

Malignant gliomas present great difficulties in treatment, with little change over the past 30 years median survival time of 15 months. Current treatment options include surgery, radiotherapy (RT), and chemotherapy. New therapies aimed at suppressing formation new vasculature (antiangiogenic treatments) or destroying formed tumor (vascular disrupting agents) show promise. This study summarizes existing knowledge regarding processes by which glioblastoma (GBM) tumors acquire resistance to antiangiogenic treatments. The discussion encompasses activation redundant proangiogenic pathways, heightened cell invasion metastasis, induced hypoxia, creation vascular mimicry channels, regulation immune microenvironment. Subsequently, we explore potential strategies overcome this resistance, such as combining other methods, personalizing treatments for each patient, focusing on therapeutic targets, incorporating immunotherapy, utilizing drug delivery systems based nanoparticles. Additionally, would like discuss limitations methods future directions enhance beneficial effects patients GBM. Therefore, review aims research outcome GBM provide a more promising opportunity thoroughly exploring mechanisms investigating novel strategies.

Language: Английский

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Magnetically Integrated Tumor–Vascular Interface System to Mimic Pro-angiogenic Endothelial Dysregulations for On-Chip Drug Testing

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(36), P. 47075 - 47088

Published: Aug. 28, 2024

The tumor–vascular interface is a critical component of the tumor microenvironment that regulates all dynamic interactions between growing and endothelial lining surrounding vasculature. In this paper, we report design development custom-engineered system for investigating early stage tumor-mediated pro-angiogenic dysfunctional behavior endothelium. Using representative cells triple negative breast cancer cell lines, established biomimetic three-dimensional tissue across mature, functional barrier using magnetically hybrid-integrated system, wherein vasculature-like features containing monolayer culture on porous microfluidic channel surfaces were attached to spheroids generated composite polymer-hydrogel microwell plate embedded in collagen matrix. Tumor-mediated microdynamics characterized by their hallmark such as loss adherens junctions, increased density, proliferation, changes spreading corroborated with YAP/TAZ nuclear translocation. We further confirm feasibility drug-mediated reversal organization through two different signaling mechanisms, namely, inhibition vascular growth factor pathway Notch pathway, thereby demonstrating utility platform rapid, prediction antiangiogenic drug efficacy. Overall, our work emphasizes importance strategic engineering approach identifying some unique, physiologically relevant aspects interface, which are otherwise difficult implement standard vitro approaches.

Language: Английский

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Vascularized platforms for investigating cell communication via extracellular vesicles

Biomicrofluidics, Journal Year: 2024, Volume and Issue: 18(5)

Published: Sept. 1, 2024

The vascular network plays an essential role in the maintenance of all organs body via regulated delivery oxygen and nutrients, as well tissue communication transfer various biological signaling molecules. It also serves a route for drug administration affects pharmacokinetics. Due to this importance, engineers have sought create physiologically relevant reproducible systems tissue, considering cell-cell extracellular matrix interaction with structural physical conditions microenvironment. Extracellular vesicles (EVs) recently emerged important carriers transferring proteins genetic material between cells organs, delivery. Vascularized platforms can be ideal system studying interactions blood vessels EVs, which are crucial understanding EV-mediated substance situations. This review summarizes recent advances vascularized platforms, standard microfluidic-based techniques EV isolation characterization, studies EVs platforms. provides insights into EV-related (patho)physiological regulations facilitates development EV-based therapeutics.

Language: Английский

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CAR-T cells are more affected than T lymphocytes by mechanical constraints: A microfluidic-based approach

Life Sciences, Journal Year: 2024, Volume and Issue: 363, P. 123335 - 123335

Published: Dec. 26, 2024

Language: Английский

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