Unveiling the versatility of gelatin methacryloyl hydrogels: a comprehensive journey into biomedical applications

Biomedical Materials, Journal Year: 2024, Volume and Issue: 19(4), P. 042008 - 042008

Published: May 20, 2024

Gelatin methacryloyl (GelMA) hydrogels have gained significant recognition as versatile biomaterials in the biomedical domain. GelMA emulate vital characteristics of innate extracellular matrix by integrating cell-adhering and metalloproteinase-responsive peptide motifs. These features enable cellular proliferation spreading within GelMA-based hydrogel scaffolds. Moreover, displays flexibility processing, it experiences crosslinking when exposed to light irradiation, supporting development with adjustable mechanical characteristics. The drug delivery landscape has been reshaped hydrogels, offering a favorable platform for controlled sustained release therapeutic actives. tunable physicochemical precise modulation kinetics release, ensuring optimal effectiveness. In tissue engineering, perform an essential role design scaffold, providing biomimetic environment conducive cell adhesion, proliferation, differentiation. Incorporating three-dimensional printing further improves its applicability developing complicated constructs spatial precision. Wound healing applications showcase bioactive dressings, fostering microenvironment regeneration. inherent biocompatibility provide efficiency closure wounds repair. stand at forefront innovation, addressing diverse challenges delivery, wound healing. This review provides comprehensive overview, in-depth understanding hydrogel's potential impact on progressing sciences.

Language: Английский

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Advances in medical polyesters for vascular tissue engineering

Discover Nano, Journal Year: 2024, Volume and Issue: 19(1)

Published: Aug. 8, 2024

Abstract Blood vessels are highly dynamic and complex structures with a variety of physiological functions, including the transport oxygen, nutrients, metabolic wastes. Their normal functioning involves close coordinated cooperation cells. However, adverse internal external environmental factors can lead to vascular damage induction various diseases, atherosclerosis thrombosis. This have serious consequences for patients, there is an urgent need innovative techniques repair damaged blood vessels. Polyesters been extensively researched used in treatment disease due their excellent mechanical properties, adjustable biodegradation time, biocompatibility. Given high complexity tissues, it still challenging optimize utilization polyesters repairing Nevertheless, they considerable potential tissue engineering range applications. summary reviews physicochemical properties polyhydroxyalkanoate (PHA), polycaprolactone (PCL), poly-lactic acid (PLA), poly(lactide-co-glycolide) (PLGA), focusing on unique applications engineering. be prepared not only as 3D scaffolds alternative grafts, but also forms such microspheres, fibrous membranes, nanoparticles deliver drugs or bioactive ingredients Finally, anticipated that further developments will occur near future, facilitate wider application these materials

Language: Английский

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Integrating Melt Electrowriting (MEW) PCL Scaffolds with Fibroblast-Laden Hydrogel toward Vascularized Skin Tissue Engineering

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101593 - 101593

Published: Feb. 20, 2025

Three-dimensional (3D) skin equivalents (SEs) are promising platforms for studying disease or assessing the safety of skin-relevant products. Vascularization, which improves functionality reconstructed skin, is one remaining hurdles in SE production that, when successfully introduced, can widen applications. Here, combining porous polycaprolactone (PCL) melt electrowritten (MEW) scaffolds with fibroblast-laden methacrylated gelatin hydrogel (GelMA), we developed SEs cellular vascular structure. The MEW were composed two layers: random fibers culturing keratinocytes to fabricate epidermis; and well-aligned shapes filled GelMA mimic dermis. Three dermal designs varying porosities pore sizes compared optimize dermis reconstruction. Within week, design bigger achieved optimal cell distribution, penetration, extracellular matrix (ECM) deposition. Additionally, Retinoic acid (RTA) was tested improving ECM To vasculature, incorporated grafts into optimized SEs. These fabricated by casting endothelial Matrigel onto small-diameter MEW-tubular structures. versatility reproducibility obtained offer a robust new tool vitro testing exploration fundamental biological processes tissue.

Language: Английский

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Protein‐Engineered Elastin Fibers as Building Blocks for The Textile‐Based Assembly of Tissue Equivalents

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(24)

Published: Feb. 10, 2024

Abstract Native tissues feature unique hierarchical designs, in which fiber units are arranged from the bottom up anisotropic patterns. The processing of biomaterials into fibers, followed by their textile‐like assembly complex patterns, is therefore a promising avenue to engineer native‐like tissue replacements. Here it shown for first time fabrication meter‐long hydrogel fibers prepared engineered elastin using microinjection system and exploiting catalyst‐free click chemistry. Given similarity native elastin, fabricated elastin‐like achieved excellent stretching (500%) recoiling performance. Moreover, scheme compatible with implementation salt‐leaching gas‐foaming approach, resulting highly porous (the kind). From translation perspective, can be autoclaved, allows sterilization long‐term storage. Human umbilical vein endothelial cells cultured on autoclaved produced confluent layer lining surface, became aligned response physiological stretching. It also that these functional assembled weaving, braiding knitting, various spatial Overall, used as building blocks reconstruction principles textile technology.

Language: Английский

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Blueprints of Architected Materials: A Guide to Metamaterial Design for Tissue Engineering

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(47)

Published: Oct. 6, 2024

Mechanical metamaterials are rationally designed structures engineered to exhibit extraordinary properties, often surpassing those of their constituent materials. The geometry metamaterials' building blocks, referred as unit cells, plays an essential role in determining macroscopic mechanical behavior. Due hierarchical design and remarkable hold significant potential for tissue engineering; however implementation the field remains limited. major challenge hindering broader use lies complexity cell fabrication. To address this gap, a comprehensive guide is presented detailing principles well-established metamaterials. geometric parameters constraints, well influence on behavior, summarized highlighting points effective Moreover, integration artificial intelligence techniques explored meta-biomaterial patient- application-specific design. Furthermore, overview current applications provided engineering, categorized by type, thereby showcasing versatility different designs matching properties target tissue. This review aims provide valuable resource engineering researchers aid field.

Language: Английский

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Effectively Guiding Cell Elongation and Alignment by Constructing Micro/Nano Hierarchical Patterned Titania on Titanium Substrate

Biotechnology and Bioengineering, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

ABSTRACT Based on the innate sensitivity of cell to substrate topographical cues, modulating cell‐directed growth behavior is crucial for promoting tissue repair and reconstruction. Although photolithography technology has been extensively employed fabricate a variety anisotropic patterned structures guide growth, it remains great challenge design high‐resolution micro/nano hierarchical directly onto medical titanium (Ti)‐based implants. Herein, we present rapid, reliable reproducible approach combining hydrothermal construct structure including micro‐strips porous composed TiO 2 nanotubes features. In vitro biological physicochemical analyses revealed that not only efficiently facilitate localization adsorption BSA molecules, but also enhances control behavior. The synergistic effect between physical limitation organizing cellular cytoskeleton at micropattern focal adhesion sits nanoscale can effectively cells maintain stable elongation alignment, even large micro‐stripe width 100 μm. This study presents promising strategy precisely multi‐level Ti using biomaterials with excellent biocompatibility. These functional hybrid micropatterns offer powerful platform regulating bioreagent behaviors in various applications engineering, regenerative medicine, drug screening, biosensors.

Language: Английский

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Artery-on-chip demonstrates smooth muscle function comparable to both healthy and diseased living tissues

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

ABSTRACT Arterial diseases affect the mechanical properties of blood vessels, which then alter their function via complex mechanisms. To develop and test effective treatments, microphysiological systems replicating mechanics a human artery are needed. Here, we establish an artery-on-chip (ARTOC) using vascular derivatives induced pluripotent stem cells (iPSCs) cultured with pulsatile flow on electrospun fibrin hydrogel. ARTOCs have mature, laminated smooth muscle that expresses robust extracellular matrix contractile proteins, contracts in response to intraluminal pressure vasoagonists, exhibits tissue comparable those small-diameter arteries. Using real-time monitoring radial distention luminal inform computational fluid dynamics modeling, show can effectively tune biomechanical cues scaffold thickness rate. We successfully these promote survival both endothelial simultaneously ARTOC. ARTOC as disease modeling platform, first use non-isogenic iPSC-derived from polycythemia patient, find significantly altered cell phenotype increased vessel wall stiffness compared controls. novel isogenic model iPSCs CRISPR-edited LMNA Hutchinson-Guilford Progeria Syndrome (LMNA G608G; HGPS ) mutation. accumulation, medial layer loss, premature senescence, loss elasticity ductility. With this work, platform for basic translational studies arterial diseases, bridging current gap linking protein expression

Language: Английский

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Biomechanical Insights into the Development and Optimization of Small-Diameter Vascular Grafts

Acta Biomaterialia, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

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Enhancing compliance and extracellular matrix properties of tissue-engineered vascular grafts through pulsatile bioreactor culture

Biomaterials Advances, Journal Year: 2025, Volume and Issue: unknown, P. 214346 - 214346

Published: May 1, 2025

Language: Английский

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Hierarchically Vascularized and Suturable Tissue Constructs created through Angiogenesis from Tissue-Engineered Vascular Grafts

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Language: Английский

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