Changes in total charge on spike protein of SARS-CoV-2 in emerging lineages DOI Creative Commons
Anže Božič, Rudolf Podgornik

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 23, 2023

Motivation: Charged amino acid residues on the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been shown to influence its binding different cell surface receptors, non-specific electrostatic interactions with environment, and structural stability conformation. It is therefore important obtain a good understanding mutations that affect total charge which arisen across SARS-CoV-2 lineages during course virus' evolution. Results: We analyse change in number ionizable acids corresponding proteins almost 2200 emerged over span pandemic. Our results show previously observed trend toward an increase positive variants concern has essentially stopped emergence early omicron variants. Furthermore, recently greater diversity terms their composition acids. also demonstrate patterns are characteristic related within broader clade division phylogenetic tree. Due ubiquity biological our findings relevant for broad range studies dealing environment. Availability: The data underlying article available online Supplementary Material.

Language: Английский

Electroactive nanofibrous membrane with antibacterial and deodorizing properties for air filtration DOI

Yuejie Dou,

Na Wang,

Shaohua Zhang

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 469, P. 134064 - 134064

Published: March 19, 2024

Language: Английский

Citations

39
Exploring advances in nanofiber-based face masks: a comprehensive review of mechanical, electrostatic, and antimicrobial functionality filtration for the removal of airborne particulate matter and pathogens DOI Creative Commons

Bao Le,

Nada Omran,

Eman Elnabawy

et al.

Emergent Materials, Journal Year: 2024, Volume and Issue: 7(3), P. 765 - 800

Published: Jan. 16, 2024

Abstract The filtration of airborne particulate matter (PM) and aerosols utilizing nonwoven fibrous materials has received significant research concern due to the continuing global pandemics, especially outbreak coronavirus disease (COVID-19), particularly for face masks as a measure personal protection. Although spun-bond or melt-blown fabrics are among pioneer in development polymer microfiber-based air filters on large scale, relatively new manufacturing processes like electrospinning solution blow spinning (SBS) gaining momentum manufacturers filter membranes. high performance nanofiber is their surface area volume ratio which increases interaction between PM improves electrostatic charge distribution electret filters, allowing enhanced capture capability based deposition. Moreover, small diameter nanofibrous breathability mask by providing slip effect, turn reduces pressure drop through membrane. This paper provides comprehensive review contemporary advances masks, detailing working mechanism involved, reviewing recent experimental studies, discussing improvements efficiency three main strategies, including mechanical antimicrobial functionality. Furthermore, prospective introduced considers synergistic combination effects mechanisms designing multifunctional structure that can efficiently wide range with higher lower drops pressure. New trends activity smart material-based membranes fight against infectious agents also described.

Language: Английский

Citations

13
Changes in total charge on spike protein of SARS-CoV-2 in emerging lineages DOI Creative Commons
Anže Božič, Rudolf Podgornik

Bioinformatics Advances, Journal Year: 2024, Volume and Issue: 4(1)

Published: Jan. 1, 2024

Abstract Motivation Charged amino acid residues on the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been shown to influence its binding different cell surface receptors, non-specific electrostatic interactions with environment, and structural stability conformation. It is therefore important obtain a good understanding mutations that affect total charge which arisen across SARS-CoV-2 lineages during course virus’ evolution. Results We analyse change in number ionizable acids corresponding proteins almost 2200 emerged over span pandemic. Our results show previously observed trend toward an increase positive variants concern has essentially stopped emergence early omicron variants. Furthermore, recently greater diversity terms their composition acids. also demonstrate patterns are characteristic related within broader clade division phylogenetic tree. Due ubiquity biological our findings relevant for broad range studies dealing environment. Availability implementation The data underlying article available Supplementary material.

Language: Английский

Citations

8
Increased preference for lysine over arginine in spike proteins of SARS-CoV-2 BA.2.86 variant and its daughter lineages DOI Creative Commons
Anže Božič, Rudolf Podgornik

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(4), P. e0320891 - e0320891

Published: April 7, 2025

The COVID-19 pandemic offered an unprecedented glimpse into the evolution of its causative virus, SARS-CoV-2. It has been estimated that since outbreak in late 2019, virus explored all possible alternatives terms missense mutations for sites polypeptide chain. Spike protein exhibits largest sequence variation particular, with many individual impacting target recognition, cellular entry, and endosomal escape virus. Moreover, recent studies unveiled a significant increase total charge on spike during initial period pandemic. While this trend recently come to halt, we perform sequence-based analysis 2665 SARS-CoV-2 variants which shows ionizable amino acids continue occur newly emerging variants, notable differences between lineages from different clades. What is more, show within can acquire positive charge, prominent preference lysine residues over arginine residues. This lysine-to-arginine ratio increased at several points evolution, most BA.2.86 sublineages, including dominant JN.1, KP.3, XEC variants. consequence structural regions now among highest viral species Coronaviridae family. impact high proteins daughter fitness remains unclear; discuss potential mechanisms could play role serve as starting point further studies.

Language: Английский

Citations

0
Spider-Net Structures in Electrospun Poly(vinyl alcohol)/Chitosan Nanofibers for Airborne Nanoparticle Filtration DOI Creative Commons
Gustavo Cardoso da Mata, Maria Sirlene Morais, Wanderley P. Oliveira

et al.

ACS Applied Nano Materials, Journal Year: 2025, Volume and Issue: unknown

Published: May 28, 2025

Language: Английский

Citations

0
Antiviral nanomedicine: Advantages, mechanisms and advanced therapies DOI

Yicheng Pu,

Chuanda Zhu, Jun Liao

et al.

Bioactive Materials, Journal Year: 2025, Volume and Issue: 52, P. 92 - 122

Published: June 5, 2025

Language: Английский

Citations

0
Modulating SARS-CoV-2 Spike Protein Reactivity through Moderate Electric Fields: A Pathway to Innovative Therapies DOI Creative Commons
Thi‐Huong Nguyen, Hanqing Wang, Liyu Chen

et al.

ACS Omega, Journal Year: 2023, Volume and Issue: 8(48), P. 45952 - 45960

Published: Nov. 21, 2023

In the quest for effective COVID-19 treatments and vaccines, traditional biochemical methods have been paramount, yet challenge of accommodating diverse viral mutants persists. Recent simulations propose an innovative physical strategy involving external electric field applied to SARS-CoV-2 spike protein, demonstrating a reduced binding potential. However, limited empirical knowledge exists regarding characteristics protein after E-field treatment. Our study addresses this gap by employing analytical techniques elucidate impact low/moderate intensity on ACE2 receptor. Through comprehensive analysis, we unveil substantial reduction in capacity validated via enzyme-linked immunosorbent assay quartz crystal microbalance experiments. Remarkably, exposure induces significant structure rearrangement, leading enhanced negative surface zeta potential confirmed dynamic light scattering. Circular dichroism spectroscopy corroborates these structural changes, showing alterations secondary structures. This provides insights into modification under pulse, potentially paving way nonbiochemical strategies mitigate reactivity opening avenues therapeutic preventive approaches against its evolving variants.

Language: Английский

Citations

2
Changes in total charge on spike protein of SARS-CoV-2 in emerging lineages DOI Creative Commons
Anže Božič, Rudolf Podgornik

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 23, 2023

Motivation: Charged amino acid residues on the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been shown to influence its binding different cell surface receptors, non-specific electrostatic interactions with environment, and structural stability conformation. It is therefore important obtain a good understanding mutations that affect total charge which arisen across SARS-CoV-2 lineages during course virus' evolution. Results: We analyse change in number ionizable acids corresponding proteins almost 2200 emerged over span pandemic. Our results show previously observed trend toward an increase positive variants concern has essentially stopped emergence early omicron variants. Furthermore, recently greater diversity terms their composition acids. also demonstrate patterns are characteristic related within broader clade division phylogenetic tree. Due ubiquity biological our findings relevant for broad range studies dealing environment. Availability: The data underlying article available online Supplementary Material.

Language: Английский

Citations

0