Frequency locked whispering evanescent resonator (FLOWER) for biochemical sensing applications

Nature Protocols, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Language: Английский

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Detection of Breath Nitric Oxide at Ppb Level Based on Multiperiodic Spectral Reconstruction Neural Network

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

As breath nitric oxide (NO) is a biomarker of respiratory inflammation, reliable techniques for the online detection ppb-level NO in exhaled are essential noninvasive diagnosis inflammation. Here, we report sensor based on multiperiodic spectral reconstruction neural network. First, method that transforms spectrum from wavelength domain to intensity proposed remove noise and interference signals spectrum. Different traditional processing domain, enhances absorption characteristics target gas while discretizing signals. This facilitates extraction Then, network built detect concentration NO. Laboratory-based results show enables (1.63–846.68 ppb) with mean absolute error (MAE), percentage (MAPE), accuracy 0.31 ppb, 0.96% 0.63%, respectively. Furthermore, an actual exhalation experiment proved capable distinguishing healthy people simulated patients, which provides way realize optical methods medical field.

Language: Английский

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Single 5-nm quantum dot detection via microtoroid optical resonator photothermal microscopy

Light Science & Applications, Journal Year: 2024, Volume and Issue: 13(1)

Published: Aug. 19, 2024

Label-free detection techniques for single particles and molecules play an important role in basic science, disease diagnostics, nanomaterial investigations. While fluorescence-based methods are tools molecule imaging, they limited by available molecular probes photoblinking photobleaching. Photothermal microscopy has emerged as a label-free imaging technique capable of detecting individual nanoabsorbers with high sensitivity. Whispering gallery mode (WGM) microresonators can confine light small volume enhanced light-matter interaction thus promising ultra-sensitive photothermal platform. Previously, microtoroid optical resonators were combined to detect 250 nm long gold nanorods 100 polymers. Here, we combine microtoroids spatially 5 diameter quantum dots (QDs) signal-to-noise ratio exceeding 10

Language: Английский

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Steviol rebaudiosides bind to four different sites of the human sweet taste receptor (T1R2/T1R3) complex explaining confusing experiments

Communications Chemistry, Journal Year: 2024, Volume and Issue: 7(1)

Published: Oct. 18, 2024

Sucrose provides both sweetness and energy by binding to Venus flytrap domains (VFD) of the heterodimeric sweet taste receptor (T1R2/T1R3). In contrast, non-caloric sweeteners such as sucralose aspartame only bind one specific domain (VFD2) T1R2, resulting in high-intensity sweetness. this study, we investigate mechanism various steviol glycosides, artificial sweeteners, a negative allosteric modulator (lactisole) at four distinct sites: VFD2, VFD3, transmembrane 2 (TMD2), TMD3 through experiments computational docking studies. Our results reveal multiple sites for tested ligands, including radiolabeled ligands. experimental evidence demonstrates that C20 carboxy terminus Gα protein can intracellular region either TMD2 or TMD3, altering GPCR affinity high-affinity state glycosides. These findings provide mechanistic understanding structure function receptor. other different (T1R2/T1R3), levels Here, authors T1R2/T1R3 studies, revealing ligands structural– correlations ligand–receptor interactions.

Language: Английский

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Towards early diagnosis and screening of Alzheimer’s disease using frequency locked whispering gallery mode microtoroids

Deleted Journal, Journal Year: 2024, Volume and Issue: 1(1)

Published: Aug. 28, 2024

Abstract Alzheimer’s disease (AD) is a form of dementia marked by amyloid plaques and neurofibrillary tangles in the brain. Amyloid beta (Aβ) an AD biomarker which linked to these tangles. Measuring Aβ levels can help with early diagnosis aid drug studies delaying dementia. This challenging, however, due low biofluids. Here we use FLOWER (frequency-locked optical whispering evanescent resonator) quantify post-mortem cerebrospinal fluid (CSF) Aβ42 control, mild cognitive impairment (MCI), participants. measures resonant wavelength shift microtoroid changes refractive index within its field. measure CSF (area under curve, AUC = 0.92) higher performance than ELISA (AUC 0.82) distinguish between control MCI samples. demonstrates FLOWER’s ability screen samples for AD.

Language: Английский

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Building blocks for nanophotonic devices and metamaterials

Chemical Communications, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

To create 3D arrangements of multiple materials in complex geometries, recent work within our lab has pursued the efficient and accurate modeling nanoparticles assembly micro- nanostructures using optical tweezers.

Language: Английский

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Fluorescence Based Trace Level Detection of Cobalt Using 2,6-bis((E)-4-chlorobenzylidene)-4-methylcyclohexan-1-one as Sensor

Journal of Fluorescence, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Language: Английский

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Optical biosensors for diagnosing neurodegenerative diseases

Deleted Journal, Journal Year: 2025, Volume and Issue: 2(1)

Published: May 2, 2025

Neurodegenerative diseases involve the progressive loss of neurons in brain and nervous system, leading to functional decline. Early detection is critical for improving outcomes advancing therapies. Optical biosensors, some which offer rapid, label-free, ultra-sensitive detection, have been applied early diagnosis drug screening. This review examines principles performance different optical biosensors used diagnosing neurodegenerative discusses potential future advancements.

Language: Английский

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Optical chemical gas sensor based on spectral autocorrelation: a method for online detection of nitric oxide and ammonia in exhaled breath

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: unknown, P. 136694 - 136694

Published: Sept. 1, 2024

Language: Английский

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Rational Design and Application of Breath Sensors for Healthcare Monitoring

ACS Sensors, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 31, 2024

Biomarkers contained in human exhaled breath are closely related to certain diseases. As a noninvasive, portable, and efficient health diagnosis method, the sensor has received considerable attention recent years for early disease screening prevention due its user-friendly easy-accessible features. Although some key challenges have been addressed, capability precisely monitor specific biomarkers of interest physiological relevance metrics is still be ascertained. In this context, we analyzed rational design advance sensors healthcare monitoring. This review begins with an introduction their sensing technologies, such as chemoresistive, humidity-sensitive, electrochemical, colorimetric principles. Then, systematic overview emerging applications screening, drunk driving inspection, apnea monitoring, condensate analysis demonstrated. Finally, discuss opportunities noninvasive With ongoing research efforts, continuous breakthrough attractive foreseeable future.

Language: Английский

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