Plugging synthetic DNA nanoparticles into the central dogma of life DOI Creative Commons
Kayla Neyra,

Shirishkant Vrijray Desai,

Divita Mathur

et al.

Chemical Communications, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Recent advancements have engineered an approach to encode genetic sequences in DNA nanoparticles, making them a promising platform for gene therapy. This article highlights design considerations of these structures optimal protein expression.

Language: Английский

Ultrasensitive Dual-Mode Biosensor for Photoelectrochemical and Differential Pulse Voltammetry Detection of Thrombin based on DNA Self-Assembly DOI

Shiliang Bi,

Changchun Chai,

Hanxiao He

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 272, P. 117132 - 117132

Published: Jan. 5, 2025

Language: Английский

Citations

0
Opportunities for DNA Origami in Electrochemical Sensing Applications DOI Creative Commons
Sergio Kogikoski,

Rafael Gonçalves Pontes,

Waldemir J. Paschoalino

et al.

ACS electrochemistry., Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 15, 2025

Language: Английский

Citations

0
Design and Characterization of a Gene-Encoding DNA Nanoparticle in a Cell-Free Transcription–Translation System DOI
Angelica Rose Galvan, Christopher M. Green, Shelby L. Hooe

et al.

ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: 7(11), P. 12891 - 12902

Published: May 30, 2024

DNA nanotechnology has made initial progress toward developing gene-encoded origami nanoparticles (NPs) that display potential utility for future gene therapy applications. However, due to the challenges involved with delivery into cells including transport through membrane, intracellular targeting, and inherent expression of nucleases along interference from other active proteins, it can be difficult more directly study effect NP design on subsequent expression. In this work, we demonstrate an approach studying gene-encoding NPs without use cells. We utilize a pure E. coli-derived cell-free transcription-translation (TXTL) system, which is composed optimized components bacterial expression, benchtop studies assess how promoter sequence in conjunction structural template affects The Renilla luciferase was first amplified single-stranded (ss) scaffold strand then folded 12-helix bundle exogenous staple strands as test platform. Using luciferase-based bioluminescence assays characterize relative protein level, found still transcribed when folded, albeit at lower rate than double-stranded segment. On comparing three variants different configurations, results indicate designed remain ssDNA form reduced NP, replacing arbitrary significantly lowers This work demonstrates power TXTL systems aid capabilities development

Language: Английский

Citations

1
Plugging synthetic DNA nanoparticles into the central dogma of life DOI Creative Commons
Kayla Neyra,

Shirishkant Vrijray Desai,

Divita Mathur

et al.

Chemical Communications, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Recent advancements have engineered an approach to encode genetic sequences in DNA nanoparticles, making them a promising platform for gene therapy. This article highlights design considerations of these structures optimal protein expression.

Language: Английский

Citations

0