Dual-Modality Imaging with a Zwitterionic Fluorescent Probe for Reversible Monitoring of Mitochondrial Membrane Potential Dynamics DOI
Fei Peng,

Xiangnan Ai,

Xiaoyu Bu

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(36), P. 47216 - 47225

Published: Aug. 30, 2024

Traditional fluorescence intensity-based probes face challenges in accurately measuring mitochondrial membrane potential (MMP) due to intramolecular quenching. In this work, we introduce a novel approach by incorporating quenching moieties within the zwitterionic probe eliminate self-quenching interference, thus, enabling real-time and precise visualization of reversible MMP changes. We synthesized fluorescent consisting silicon-rhodamine (SiR) that was hydroxyl-substituted on bay position perylene diimides (PDIs) connected via polyethylene glycol (PEG) linker. The lipophilic cationic SiR facilitates entry PDI into mitochondria, where alkaline pH environment (pH = 8.0) ionizes hydroxyl negatively charged species, affecting efficiency depending distance between regulated MMP. rigid aromatic ring strong hydrophobic interactions with lipid bilayer, along inhibitory effect internalization, ensure retention mitochondria. As decreases, shifts outward, reducing phenolic anions restoring fluorescence. Conversely, as increases, moves inward, intensifying ions fluorescence, monitoring This strategy overcomes limitations traditional probes, providing new avenue for

Language: Английский

Dual-Modality Imaging with a Zwitterionic Fluorescent Probe for Reversible Monitoring of Mitochondrial Membrane Potential Dynamics DOI
Fei Peng,

Xiangnan Ai,

Xiaoyu Bu

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(36), P. 47216 - 47225

Published: Aug. 30, 2024

Traditional fluorescence intensity-based probes face challenges in accurately measuring mitochondrial membrane potential (MMP) due to intramolecular quenching. In this work, we introduce a novel approach by incorporating quenching moieties within the zwitterionic probe eliminate self-quenching interference, thus, enabling real-time and precise visualization of reversible MMP changes. We synthesized fluorescent consisting silicon-rhodamine (SiR) that was hydroxyl-substituted on bay position perylene diimides (PDIs) connected via polyethylene glycol (PEG) linker. The lipophilic cationic SiR facilitates entry PDI into mitochondria, where alkaline pH environment (pH = 8.0) ionizes hydroxyl negatively charged species, affecting efficiency depending distance between regulated MMP. rigid aromatic ring strong hydrophobic interactions with lipid bilayer, along inhibitory effect internalization, ensure retention mitochondria. As decreases, shifts outward, reducing phenolic anions restoring fluorescence. Conversely, as increases, moves inward, intensifying ions fluorescence, monitoring This strategy overcomes limitations traditional probes, providing new avenue for

Language: Английский

Citations

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