An Anti-Oxidative Bioink for Cartilage Tissue Engineering Applications DOI Creative Commons
Xin Chen,

Mengni Yang,

Zheng Zhou

et al.

Journal of Functional Biomaterials, Journal Year: 2024, Volume and Issue: 15(2), P. 37 - 37

Published: Feb. 2, 2024

Since chondrocytes are highly vulnerable to oxidative stress, an anti-oxidative bioink combined with 3D bioprinting may facilitate its applications in cartilage tissue engineering. We developed methacrylate-modified rutin (RTMA) as additional bioactive component and glycidyl methacrylate silk fibroin a biomaterial component. Bioink containing 0% RTMA was used the control sample. Compared hydrogel samples produced bioink, solidified bioinks displayed similar porous microstructure, which is suitable for cell adhesion migration, transportation of nutrients wastes. Among photo-cured prepared sample 1 mg/mL (RTMA-1) showed good degradation, promising mechanical properties, best cytocompatibility, it selected further investigation. Based on results tests, RTMA-1 exhibited printability high shape fidelity. The demonstrated that reduced intracellular stress encapsulated under H2O2 stimulation, from upregulation COLII AGG downregulation MMP13 MMP1. By using vitro vivo our data suggest significantly enhanced regeneration maturation compared indicating this can be engineering future.

Language: Английский

Biomedical potentials of alginate via physical, chemical, and biological modifications DOI
Qiaoqiao Li, Dong Xu,

Qin-Wei Dong

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 277, P. 134409 - 134409

Published: Aug. 7, 2024

Language: Английский

Citations

14
Continuous‐Gradient Mineralized Hydrogel Synthesized via Gravitational Osmosis for Osteochondral Defect Repair DOI Open Access

Rongtai Sun,

Qiang Zhang, Congcong Yu

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 2, 2024

Abstract The repair of hierarchical osteochondral defects requires complex gradient reconstruction at different levels, with continuous‐gradient mineralization being crucial. Strategies for achieving have rarely been reported. Here, a mineralized hydrogel is prepared using simple gravitational osmosis ≈2 nm amorphous calcium phosphate nanocluster (ACPC) suspension into composite organic frameworks regeneration. During infiltration, ACPC underwent gradual mineralization, resulting in the spontaneous formation hydroxyapatite (HAP). aligned closely normal structure, thereby effectively promoting cartilage and subchondral bone. In layer, compounds improved oxidative stress environment induced by injury complemented extracellular matrix cartilage. bone HAP from continuously intracellular accumulation mesenchymal stem cells (MSCs), activating calcium/calmodulin‐dependent protein kinase 2 (CaMK2) osteogenic differentiation MSCs through signaling pathway. Ultimately, compared nongradient hydrogel, this innovative method exhibited significantly enhanced regeneration capabilities defects, as evidenced metrics, such volume/tissue volume (BV/TV), trabecular thickness (Tb.Th), number (Tb.N). This holistic strategy provides hope field repair.

Language: Английский

Citations

9
Harnessing Theraoenergetics for Cartilage Regeneration: Development of a Therapeutic and Bioenergetic Loaded Janus Nanofiber Reinforced Hydrogel Composite for Cartilage Regeneration DOI
Shivani Chaudhary, Sandeep Jain,

Doyel Ghosal

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Advancements in tissue engineering and regenerative medicine have highlighted different strategies of designing hydrogels to replicate the intricate structure cartilage extracellular matrix (ECM) for effective regeneration. However, despite efforts meet elevated structural mechanical demands repair, researchers often overlook challenging environmental conditions at damaged sites such as inflammation, hypoxia, limited availability nutrients energy, which are critical supporting The insufficient oxygen, nutrient availability, oxidative stress avascular limit phosphorylation-mediated bioenergetics cells needed energy required anabolic biosynthesis, cell division, migration during repair. Thus, there is a need develop an advanced approach engineer unique hydrogel system that not only provides properties but also integrates therapeutics (like anti-inflammatory, reactive oxygen species (ROS) scavenging) demand) into hydrogel, may offer holistic solution repairing defects under harsh microenvironment. In this study, we engineered innovative new class theraoenergetic by reinforcing Janus nanofiber (JNF) carrying therapeutic (MgO) bioenergetic (polyglutamic acid), PGA) components dual network photo-crosslinkable hydrogel. Reinforcement JNF microfragments photo-crosslinking synthesized gelatin methacryloyl (GelMA) carboxymethyl chitosan (CMCh) enhances hydrogel's 800% withstand load ensures controlled release magnesium, PGA over 30 days. Co-delivery magnesium with helped synergistically reduce intracellular ROS inflammatory markers IL-6 TNF-α, providing supportive environment enhancing mitochondrial metabolism leading active proliferation chondrogenic differentiation stem deposit glycosaminoglycan (GAG)-rich regenerate cartilage. developed represents promising regenerating microenvironment treat osteoarthritis, rising global health burden.

Language: Английский

Citations

0
An Injectable PEG/Diacerein‐Based Anti‐Inflammatory Hydrogel for Promoting Cartilage Regeneration: An In Vivo Study DOI Creative Commons

Jing Mi,

Linghui Meng,

Yutian Wang

et al.

Macromolecular Materials and Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Abstract Cartilage defects are common joint disorders that, if left untreated, may progress to severe degenerative conditions. Inflammatory response plays a critical role in the pathogenesis of cartilage damage. Hydrogels incorporating diacerein, an anti‐inflammatory drug used clinical settings, can mitigate inflammation that impairs repair. It is hypothesized direct injection hydrogel scaffold combining diacerein and polydopamine into defect sites enhance localized treatment, reduce surgical risks, expedite recovery. Therefore, this study, infused with developed investigate its efficacy for restoration. By crosslinking poly(ethylene glycol) diacrylate, four‐arm polyethylene glycol‐functionalized hyaluronic acid, polydopamine, injectable superior properties achieved. In vitro evaluations confirm mechanical strength biocompatibility hydrogel, vivo studies demonstrate effectiveness repair activity rat model. These findings indicate hydrogels promising materials addressing advancing tissue engineering biological implantation strategies.

Language: Английский

Citations

0
Exploring the Impact of Volumetric Additive Manufacturing of Photo-crosslinkable Gelatin on Mesenchymal Stromal Cell Behavior and Differentiation DOI Open Access
Nele Pien, Bryan Bogaert, Marguerite Meeremans

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

Abstract This study explores the application of photo-crosslinkable gelatin hydrogels (constituting GelSH and GelNB) for biofabrication using volumetric additive manufacturing (VAM). Three variants were prepared, yielding degree substitutions (DS) 39%, 54%, 63%, GelNB with a DS 60%, alongside GelNB-GelSH formulations at concentrations 5, 7.5, 10% (w/v). Physico-chemical analyses confirmed that these variations led to differences in mass swelling ratio mechanical properties. VAM enabled fabrication complex, high-fidelity 3D structures from optimized formulations, demonstrating its capacity encapsulate mesenchymal stromal cells (MSCs) within biomimetic matrix. Compared film casted constructs, VAM-printed scaffolds exhibited significantly higher ALP activity calcium deposition, confirming effective osteogenesis. Conversely, chondrogenic adipogenic differentiation more pronounced groups due their lower crosslinking density compressive elastic modulus, emphasizing role environment guiding cell differentiation. highlights potential produce complex functional hydrogel tissue engineering, lays groundwork future work on finetuning bioresin enhance across multiple lineages various engineering applications, including softer tissues. Figure investigates (GelSH (VAM) biofabrication. Different influenced produced encapsulating cells, enhancing osteogenesis compared film-cast constructs. The findings highlight VAM’s optimizing bioresins multi-lineage differentiation,

Language: Английский

Citations

0
Preparation and characterization of photocrosslinked bletilla striata polysaccharide/hyaluronic acid injectable hydrogels DOI

Yilong Wei,

Yanan Jiang,

Doudou Ma

et al.

International Journal of Polymeric Materials, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 15

Published: March 24, 2025

Language: Английский

Citations

0
An Anti-Oxidative Bioink for Cartilage Tissue Engineering Applications DOI Creative Commons
Xin Chen,

Mengni Yang,

Zheng Zhou

et al.

Journal of Functional Biomaterials, Journal Year: 2024, Volume and Issue: 15(2), P. 37 - 37

Published: Feb. 2, 2024

Since chondrocytes are highly vulnerable to oxidative stress, an anti-oxidative bioink combined with 3D bioprinting may facilitate its applications in cartilage tissue engineering. We developed methacrylate-modified rutin (RTMA) as additional bioactive component and glycidyl methacrylate silk fibroin a biomaterial component. Bioink containing 0% RTMA was used the control sample. Compared hydrogel samples produced bioink, solidified bioinks displayed similar porous microstructure, which is suitable for cell adhesion migration, transportation of nutrients wastes. Among photo-cured prepared sample 1 mg/mL (RTMA-1) showed good degradation, promising mechanical properties, best cytocompatibility, it selected further investigation. Based on results tests, RTMA-1 exhibited printability high shape fidelity. The demonstrated that reduced intracellular stress encapsulated under H2O2 stimulation, from upregulation COLII AGG downregulation MMP13 MMP1. By using vitro vivo our data suggest significantly enhanced regeneration maturation compared indicating this can be engineering future.

Language: Английский

Citations

1