Journal of Neurorestoratology,
Journal Year:
2024,
Volume and Issue:
12(3), P. 100131 - 100131
Published: June 13, 2024
Restoring
function
to
peripheral
nerves
with
a
gap
is
challenging,
<50%
of
patients
undergoing
nerve
repair
surgery
recovering
function.
Sensory
grafts
(autografts)
are
the
clinical
"gold
standard"
for
bridging
gaps
restore
sensory
and
motor
They
have
significant
limitations
meaningful
only
across
short
when
repairs
performed
soon
after
trauma
young.
When
value
any
these
variables
large,
extent
recovery
decreases
precipitously,
two
or
all
simultaneously
there
little
no
recovery.
The
restored
has
not
increased
in
almost
70
years.
Thus,
novel
techniques
needed
that
enhance
both
percentage
who
recover
This
paper
reviews
autografts
other
materials
used
nerves.
It
also
examines
autologous
platelet-rich
plasma
(PRP),
promising
technique
induces
settings
where
ineffective,
including
values
three
large.
Biomacromolecules,
Journal Year:
2024,
Volume and Issue:
25(4), P. 2156 - 2221
Published: March 20, 2024
Tissue
engineering
for
injured
tissue
replacement
and
regeneration
has
been
a
subject
of
investigation
over
the
last
30
years,
there
considerable
interest
in
using
additive
manufacturing
to
achieve
these
goals.
Despite
such
efforts,
many
key
questions
remain
unanswered,
particularly
area
biomaterial
selection
applications
as
well
quantitative
understanding
process
science.
The
strategic
utilization
biological
macromolecules
provides
versatile
approach
meet
diverse
requirements
3D
printing,
printability,
buildability,
biocompatibility.
These
molecules
play
pivotal
role
both
physical
chemical
cross-linking
processes
throughout
biofabrication,
contributing
significantly
overall
success
printing
process.
Among
several
bioprintable
materials,
gelatin
methacryloyl
(GelMA)
widely
utilized
applications,
with
some
degree
success.
In
this
context,
review
will
discuss
bioengineering
approaches
identify
gelation
strategies
that
are
appropriate
control
rheology,
buildability
inks.
This
focus
on
GelMA
structural
(scaffold)
different
tissues
potential
carrier
vehicle
transport
living
cells
their
maintenance
viability
physiological
system.
Recognizing
importance
printability
toward
shape
fidelity
biophysical
properties,
major
qualitative
impact
factors,
including
microrheological,
viscoelastic,
gelation,
shear
thinning
properties
inks,
parameters,
particular,
reference
extrusion
GelMA-based
Specifically,
we
emphasize
possibilities
regulate
mechanical,
swelling,
biodegradation,
cellular
functionalities
bio(material)
by
hybridization
techniques,
synthetic
natural
biopolymers,
inorganic
nanofillers,
microcarriers.
At
close,
possibility
integration
experimental
data
sets
artificial
intelligence/machine
learning
is
emphasized
predict
fidelity,
or
inks
clinically
relevant
tissues.
ACS Applied Bio Materials,
Journal Year:
2024,
Volume and Issue:
7(5), P. 2809 - 2835
Published: April 11, 2024
Three-dimensional
(3D)
bioprinting
of
hydrogels
with
a
wide
spectrum
compositions
has
been
widely
investigated.
Despite
such
efforts,
comprehensive
understanding
the
correlation
among
process
science,
buildability,
and
biophysical
properties
for
targeted
clinical
application
not
developed
in
scientific
community.
In
particular,
quantitative
analysis
across
entire
developmental
path
3D
extrusion
scaffolds
is
reported.
present
work,
we
addressed
this
gap
by
using
investigated
biomaterials,
as
gelatin
methacryloyl
(GelMA),
model
system.
Using
extensive
experiments
analysis,
analyzed
how
individual
components
methacrylated
carboxymethyl
cellulose
(mCMC),
needle-shaped
nanohydroxyapatite
(nHAp),
poly(ethylene
glycol)diacrylate
(PEGDA)
GelMA
baseline
matrix
multifunctional
bioink
can
influence
properties,
printability,
cellular
functionality.
The
complex
interplay
biomaterial
ink
formulations,
viscoelastic
printability
toward
large
structure
buildability
(structurally
stable
cube
15
mm
edge)
explored.
Intriguingly,
incorporation
PEGDA
into
GelMA/mCMC
offered
improved
compressive
modulus
(∼40-fold),
reduced
swelling
ratio
(∼2-fold),
degradation
rates
(∼30-fold)
compared
to
pristine
GelMA.
microstructural
pore
architecture,
cytocompatibility
also
established
inks.
These
photopolymerizable
bio(material)inks
served
platform
growth
development
bone
cartilage
when
human
mesenchymal
stem
cells
(hMSCs)
are
either
seeded
on
two-dimensional
(2D)
substrates
or
encapsulated
scaffolds.
Taken
together,
study
unequivocally
establishes
significant
step
forward
broad
shape-fidelity
compliant
emphasizes
need
invoking
more
establishing
process-microstructure–property
correlation.
Neurology International,
Journal Year:
2025,
Volume and Issue:
17(2), P. 23 - 23
Published: Feb. 6, 2025
Peripheral
nerve
injuries
are
common
complications
in
surgical
and
dental
practices,
often
resulting
functional
deficiencies
reduced
quality
of
life.
Current
treatment
choices,
such
as
autografts,
have
limitations,
including
donor
site
morbidity
suboptimal
outcomes.
Adipose-derived
stem
cells
(ADSCs)
shown
assuring
regenerative
potential
due
to
their
accessibility,
ease
harvesting
propagation,
multipotent
properties.
This
review
investigates
the
therapeutic
ADSCs
peripheral
regeneration,
focusing
on
use
bioengineered
conduits
supportive
microenvironments.
The
analysis
is
constructed
published
case
reports,
organized
reviews,
clinical
trials
from
Phase
I
III
that
investigate
managing
injuries,
emphasizing
both
orofacial
applications.
findings
highlight
advantages
promoting
secretion
angiogenic
neurotrophic
factors,
support
for
cellular
persistence,
supplementing
scaffold-based
tissue
repair.
capabilities
offer
a
novel
approach
augmenting
repair
recovery.
accessibility
adipose
minimally
invasive
nature
ADSC
further
encourage
its
prospective
application
an
autologous
cell
source
medicine.
Future
research
needed
ascertain
standardized
protocols
optimize
outcomes,
paving
way
become
mainstay
regeneration.
Biomaterials Research,
Journal Year:
2025,
Volume and Issue:
29
Published: Jan. 1, 2025
The
repair
of
peripheral
nerve
injury
(PNI)
presents
a
multifaceted
and
protracted
challenge,
with
current
therapeutic
approaches
failing
to
achieve
optimal
outcomes,
thereby
not
satisfying
the
considerable
clinical
demand.
advent
tissue
engineering
has
led
growing
body
experimental
evidence
indicating
that
synergistic
application
conduits,
which
provide
structural
guidance,
alongside
biological
signals
derived
from
exosomes
stem
cells,
yields
superior
results
for
PNI
compared
isolated
interventions.
This
combined
approach
holds
great
promise
application.
In
this
review,
we
present
latest
advancements
in
treatment
through
integration
cells
or
conduits.
We
have
addressed
inadequate
efficiency
conjunction
conduits
3
perspectives:
enhancing
exosomes,
improving
incorporating
physical
stimulation.
Macromolecular Bioscience,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 25, 2025
Current
clinical
interventions
for
large
peripheral
nerve
gap
injuries
are
limited.
Bioprinting
provides
opportunities
to
develop
tissue
engineered
constructs
that
provide
a
biomimetic
environment
guide
regeneration.
However,
hydrogels
cell-instructive,
mechanically
compliant,
and
have
an
appropriate
biodegradation
profile
guidance
conduit
applications
In
this
study,
photocrosslinkable
gelatin
methacryloyl
(GelMA)
polyethylene
glycol
diacrylate
(PEGDA)
hybrid
bioink
is
developed.
The
role
of
PEGDA
molecular
weight
concentration
in
tuning
the
hydrogel
physicochemical
biological
properties
evaluated.
modulated
network
structure
dependent
manner.
A
lower
high
induced
crosslinking
density
thus
improving
compressive
modulus,
swelling,
slower
degradation
profile.
bioinks
showed
good
printability
able
fabricate
multi-layer
with
shape
fidelity
flexibility.
SH-SY5Y
cells
maintained
cell
viability
after
bioprinting
all
bioinks.
limited
metabolic
activity
spreading
GelMA/PEGDA
both
weight.
This
preliminary
study
on
use
specific
weights
concentrations
cells.
Journal of Biomaterials Applications,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 3, 2025
The
last
two
decaes
have
witnessed
significant
efforts
to
develop
gelatin/alginate
based
scaffolds
using
variants
of
3D
printing
techniques.
However,
their
biocompatibility
for
regenerating
complex
soft
tissues
remains
insufficiently
explored.
Addressing
this
gap,
we
fabricated
3D-printed
alginate-gelatin
(3A5G)
and
nanocellulose-reinforced
(3A5G1C)
hydrogel
with
clinically
relevant
dimensions
(15
mm
diameter,
5
height)
the
host
tissue
responses
were
critically
analyzed.
distinct
advantages
nanocellulose
in
modulating
mechanical
strength,
viscoelasticity,
swelling,
degradation
characteristics
established
our
prior
studies.
This
investigation
aimed
comprehensively
evaluate
foreign
body
response
these
a
rat
model.
animals
exhibited
healthy
metabolic
activity,
evidenced
by
progressive
weight
gain,
localized
healing,
normal
mobility
over
30
days.
Histological
analyses
could
not
reveal
any
adverse
immune
reaction
at
7-
or
30-days,
post-implantation.
Hematological
serum
biochemical
assessments
indicated
progression
from
acute
(7
days)
sub-acute
(30
inflammation,
following
subcutaneous
implantation,
without
signature
systemic
toxicity.
Immune
marker
evaluation
(TNF-α,
CD-8,
CD-68,
COX-2,
IL-6)
confirmed
absence
pathological
responses,
even
incorporation.
Immunohistochemical
analysis
CD31
staining
demonstrated
enhanced
vascularization
both
7
toxicity
scaffold
products
favorable
outcomes
underline
potential
regeneration.
incorporation
further
scaffolds'
functional
performance,
particularly
promoting
vascularization,
positioning
them
as
promising
candidates
engineering
applications.
