Deleted Journal,
Journal Year:
2023,
Volume and Issue:
2(2), P. 37 - 43
Published: Dec. 29, 2023
Cross-linked
enzymes
aggregate
(CLEA)
is
a
versatile
carrier
free-immobilization
technique
that
has
gained
much
attention
in
the
development
of
biocatalyst
technology.
However,
there
no
precise
and
accurate
method
using
this
will
lead
to
an
expected
outcome
meets
requirements
industrial
standard.
Therefore,
objective
study
investigate
effect
few
methods
executing
cross-linked
enzyme
aggregates
approach
maltogenic
amylase
by
measuring
activity
recovery
developed
CLEA.
Some
factors
are
considered
developing
methodologies
interaction
between
cross-linkers
enzymes,
size
substrate
diffusion.
The
addition
precipitant
cross-linking
agent
steps
been
manipulated
four
different
were
developed.
Based
on
results,
Method
2
showed
highest
(57.9%)
whilst
4
gave
lowest
(15.7%).
improvised
removed
supernatant
after
centrifugation
before
proceeding
step.
characterization
such
as
morphological
Fourier
Transform-Infrared
Spectroscopy
was
also
determined.
In
conclusion,
best
most
productive
preparation
determined
based
recovery.
Catalysts,
Journal Year:
2024,
Volume and Issue:
14(1), P. 84 - 84
Published: Jan. 19, 2024
Biocatalysis
holds
immense
potential
for
pharmaceutical
development
as
it
enables
synthetic
routes
to
various
chiral
building
blocks
with
unparalleled
selectivity.
Therein,
solvent
and
water
use
account
a
large
contribution
the
environmental
impact
of
reactions.
In
spirit
Green
Chemistry,
transition
from
traditional
highly
diluted
aqueous
systems
intensified
non-aqueous
media
overcome
limitations
(e.g.,
shortages,
recalcitrant
wastewater
treatments,
low
substrate
loadings)
has
been
observed.
Benefiting
spectacular
advances
in
enzyme
stabilization
techniques,
plethora
biotransformations
non-conventional
have
established.
Deep
eutectic
solvents
(DESs)
emerge
sort
(potentially)
greener
medium
increasing
biocatalysis.
This
review
discusses
state-of-the-art
DESs
focus
on
biocatalytic
pathways
synthesis
active
ingredients
(APIs).
Representative
examples
different
classes
are
discussed,
together
critical
vision
discussing
prospects
using
Proceedings of the National Academy of Sciences,
Journal Year:
2024,
Volume and Issue:
121(34)
Published: Aug. 12, 2024
Mutations
in
protein
active
sites
can
dramatically
improve
function.
The
site,
however,
is
densely
packed
and
extremely
sensitive
to
mutations.
Therefore,
some
mutations
may
only
be
tolerated
combination
with
others
a
phenomenon
known
as
epistasis.
Epistasis
reduces
the
likelihood
of
obtaining
improved
functional
variants
slows
natural
lab
evolutionary
processes.
Research
has
shed
light
on
molecular
origins
epistasis
its
role
shaping
trajectories
outcomes.
In
addition,
sequence-
AI-based
strategies
that
infer
epistatic
relationships
from
mutational
patterns
or
experimental
evolution
data
have
been
used
design
variants.
recent
years,
combinations
such
approaches
atomistic
calculations
successfully
predicted
highly
combinatorial
sites.
These
were
thousands
active-site
variants,
demonstrating
that,
while
our
understanding
remains
incomplete,
determinants
are
critical
for
accurate
now
sufficiently
understood.
We
conclude
space
explored
by
expanded
enhance
activities
discover
new
ones.
Furthermore,
opens
way
systematically
exploring
sequence
structure
impacts
function,
deepening
control
over
activity.
JBIC Journal of Biological Inorganic Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 18, 2024
Abstract
Examples
of
how
metalloproteins
feature
in
electron
transfer
processes
biological
systems
are
reviewed.
Attention
is
focused
on
the
transport
chains
cellular
respiration
and
photosynthesis,
that
directly
couple
to
a
chemical
reaction.
Brief
mention
also
made
extracellular
transport.
While
covering
highlights
recent
current
literature,
this
review
aimed
primarily
at
introducing
senior
undergraduate
novice
postgraduate
student
important
aspect
bioinorganic
chemistry.
Graphical
abstract
Energetic Materials Frontiers,
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 1, 2024
Recent
years
have
witnessed
significant
advancements
in
methodologies
and
techniques
for
the
synthesis
of
energetic
materials,
which
are
expected
to
shape
future
manufacturing
applications.
Techniques
including
continuous
flow
chemistry,
electrochemical
synthesis,
microwave-assisted
biosynthesis
been
extensively
employed
pharmaceutical
fine
chemical
industries
and,
gratifyingly,
found
broader
This
review
comprehensively
introduces
recent
utilization
these
emerging
techniques,
aiming
provide
a
catalyst
development
novel
green
methods
synthesizing
materials.
Pharmaceuticals,
Journal Year:
2024,
Volume and Issue:
17(2), P. 221 - 221
Published: Feb. 7, 2024
Chemists
in
the
medicinal
chemistry
field
are
constantly
searching
for
alternatives
towards
more
sustainable
and
eco-friendly
processes
design
synthesis
of
drug
candidates.
The
pharmaceutical
industry
is
one
most
polluting
industries,
having
a
high
E-factor,
which
driving
adoption
not
only
new
candidates,
but
also
production
well-established
active
ingredients.
Deep
eutectic
systems
(DESs)
have
emerged
as
greener
alternative
to
ionic
liquids,
their
potential
substitute
traditional
organic
solvents
discovery
has
raised
interest
among
scientists.
With
use
DESs
solvents,
become
attractive
terms
eco-friendliness
recyclability.
Furthermore,
they
might
be
effective
through
making
process
simpler,
faster,
with
maximum
efficiency.
This
review
will
focused
on
role
application
deep
discovery,
using
biocatalytic
chemical
reactions,
environmentally
benign
solvents.
herein
we
show
that
DESs,
if
used
industry,
may
significant
effect
lowering
costs
decreasing
impact
this
quality
environment.
European Journal of Organic Chemistry,
Journal Year:
2024,
Volume and Issue:
27(8)
Published: Jan. 5, 2024
Abstract
The
constant
demand
for
sustainable
approaches
to
pharmaceutical
synthesis
has
thrived
the
enzyme
catalysis
(biocatalysis)
field.
most
sought‐after
features
like
high
specificity,
environmentally
benign,
and
biodegradable
nature
have
rendered
enzymatic
processes
predominantly
eco‐friendly.
Owing
these
merits,
biocatalysis
is
now
being
developed
extensively
exploit
their
applications
in
designing
alternate
synthetic
routes
manufacturing
pharmaceuticals,
line
with
green
chemistry
principles.
Further,
combining
multiple
enzymes
one‐pot
a
highly
efficient
methodology
as
it
eliminates
intermediate
isolation,
shifts
equilibrium
forward
direction,
proceeds
ambient
aqueous
conditions.
This
review
highlights
importance
of
recently
multienzyme
cascade
pharmaceutically
important
bioactive
molecules
by
relating
them
well‐known
chemical
route.
Advanced Synthesis & Catalysis,
Journal Year:
2024,
Volume and Issue:
366(11), P. 2461 - 2467
Published: March 28, 2024
Abstract
We
described
the
protein
engineering
of
halohydrin
dehalogenase
HheG
to
enhance
its
R
enantioselectivity
for
synthesis
chiral
4‐aryloxazolidinones
via
α‐regioselective
ring‐opening
aryl
epoxides
with
cyanate.
Additionally,
we
achieved
inversion
regioselectivity
and
enantioselectivity,
resulting
in
a
variant
tailored
5‐aryloxazolidinones
β‐regioselective
S
‐enantioselective
Leveraging
these
engineered
mutants,
developed
biocatalytic
platform
capable
both
(up
47%
yield,
98%
ee
,
99%
regioselectivity)
46%
>99%
regioselectivity).
Furthermore,
collaborative
approach
utilizing
two
regio‐
enantioselective
mutants
has
been
demonstrated
enable
simultaneous
5‐aryloxazolidinones.
