Biotechnology and Genetic Engineering Reviews,
Journal Year:
2022,
Volume and Issue:
38(1), P. 1 - 32
Published: Jan. 2, 2022
Worldwide,
environmental
pollution
due
to
a
complex
mixture
of
xenobiotics
has
become
serious
concern.
Several
xenobiotic
compounds
cause
contamination
their
severe
toxicity,
prolonged
exposure,
and
limited
biodegradability.
From
the
past
few
decades,
microbial-assisted
degradation
(bioremediation)
pollutants
evolved
as
most
effective,
eco-friendly,
valuable
approach.
Microorganisms
have
unique
metabolism,
capability
genetic
modification,
diversity
enzymes,
various
pathways
necessary
for
bioremediation
process.
Microbial
is
effective
but
slow
process
that
limits
its
application
in
bioremediation.
However,
study
microbial
enzymes
gaining
global
importance.
huge
ability
transform
contaminants
into
non-toxic
forms
thereby
reduce
pollution.
Recently,
advanced
techniques,
including
metagenomics,
proteomics,
transcriptomics,
metabolomics
are
effectively
utilized
characterization,
metabolic
machinery,
new
proteins,
genes
microorganisms
involved
These
molecular
techniques
provide
thorough
understanding
structural
functional
aspects
microorganisms.
This
review
gives
brief
note
on
impact
environment.
Particular
attention
will
be
devoted
class
such
cytochrome
P450,
dehydrogenase,
laccase,
hydrolase,
protease,
lipase,
etc.
capable
converting
these
innocuous
products.
attempts
deliver
knowledge
role
biodegradation
pollutants,
along
with
use
technologies
like
recombinant
DNA
technology
Omics
approaches
make
more
robust
effective.
Critical Reviews in Biotechnology,
Journal Year:
2022,
Volume and Issue:
43(2), P. 227 - 241
Published: Feb. 6, 2022
Natural
products,
a
chemically
and
structurally
diverse
class
of
molecules,
possess
wide
spectrum
biological
activities,
have
been
used
therapeutically
for
millennia,
provided
many
lead
compounds
the
development
synthetic
drugs.
Cytochrome
P450
enzymes
(P450s,
CYP)
are
widespread
in
nature
involved
biosynthesis
natural
products.
P450s
heme-containing
that
use
molecular
oxygen
hydride
donor
NAD(P)H
(coupled
via
enzymic
redox
partners)
to
catalyze
insertion
into
C-H
bonds
regio-
stereo-selective
manner,
effecting
hydroxylation
several
other
reactions.
With
rapid
systems
biology,
numerous
novel
identified
but
there
still
challenges
efficient
heterologous
expression
active
P450s.
This
review
covers
recent
developments
research
development,
including
properties
functions
P450s,
discovery
mining
modification
screening
mutants,
improved
microbial
hosts,
whole-cell
transformation
with
current
applications
resource
provides
solid
foundation
application
highly
stable
cell
factories
biosynthesize
Natural Product Reports,
Journal Year:
2021,
Volume and Issue:
38(8), P. 1469 - 1505
Published: Jan. 1, 2021
Cytochrome
P450s,
laccases,
and
intermolecular
[4
+
2]
cyclases,
along
with
other
enzymes
were
utilized
to
catalyze
varied
dimerization
of
matured
natural
products
so
as
create
the
structural
diversity
complexity
in
microorganisms.
Biotechnology and Genetic Engineering Reviews,
Journal Year:
2022,
Volume and Issue:
38(1), P. 1 - 32
Published: Jan. 2, 2022
Worldwide,
environmental
pollution
due
to
a
complex
mixture
of
xenobiotics
has
become
serious
concern.
Several
xenobiotic
compounds
cause
contamination
their
severe
toxicity,
prolonged
exposure,
and
limited
biodegradability.
From
the
past
few
decades,
microbial-assisted
degradation
(bioremediation)
pollutants
evolved
as
most
effective,
eco-friendly,
valuable
approach.
Microorganisms
have
unique
metabolism,
capability
genetic
modification,
diversity
enzymes,
various
pathways
necessary
for
bioremediation
process.
Microbial
is
effective
but
slow
process
that
limits
its
application
in
bioremediation.
However,
study
microbial
enzymes
gaining
global
importance.
huge
ability
transform
contaminants
into
non-toxic
forms
thereby
reduce
pollution.
Recently,
advanced
techniques,
including
metagenomics,
proteomics,
transcriptomics,
metabolomics
are
effectively
utilized
characterization,
metabolic
machinery,
new
proteins,
genes
microorganisms
involved
These
molecular
techniques
provide
thorough
understanding
structural
functional
aspects
microorganisms.
This
review
gives
brief
note
on
impact
environment.
Particular
attention
will
be
devoted
class
such
cytochrome
P450,
dehydrogenase,
laccase,
hydrolase,
protease,
lipase,
etc.
capable
converting
these
innocuous
products.
attempts
deliver
knowledge
role
biodegradation
pollutants,
along
with
use
technologies
like
recombinant
DNA
technology
Omics
approaches
make
more
robust
effective.
