The Journal of Physical Chemistry B,
Journal Year:
2023,
Volume and Issue:
127(14), P. 3208 - 3222
Published: April 3, 2023
Photoisomerization
kinetics
of
the
near-infrared
(NIR)
fluorophore
Sulfo-Cyanine7
(SCy7)
was
studied
by
a
combination
fluorescence
correlation
spectroscopy
(FCS)
and
transient
state
(TRAST)
excitation
modulation
spectroscopy.
A
photoisomerized
with
redshifted
emission
identified,
consistent
three-state
photoisomerization
model.
Combining
TRAST
spectrofluorimetry
(spectral-TRAST)
further
confirmed
an
excitation-induced
redshift
in
spectrum
SCy7.
We
show
how
this
red-emissive
contributes
to
blinking
different
bands
NIR
cyanine
dyes,
it
can
influence
single-molecule,
super-resolution,
as
well
Förster
resonance
energy
transfer
(FRET)
multicolor
readouts.
Since
also
be
populated
at
moderate
intensities,
more
broadly
readouts,
readouts
not
relying
on
high
conditions.
However,
additional
its
photodynamics,
identified
characterized
work,
used
strategy
push
dyes
into
enhance
photosensitization
nanoparticles
absorption
spectra
NIR.
Finally,
we
that
SCy7
formation
photoisomer
depend
strongly
local
environmental
conditions,
such
viscosity,
polarity,
steric
constraints,
which
suggests
use
other
sensors.
Such
information
monitored
TRAST,
NIR,
low
autofluorescence
scattering
conditions
broad
range
samples
experimental
Chemical Society Reviews,
Journal Year:
2023,
Volume and Issue:
52(20), P. 7197 - 7261
Published: Jan. 1, 2023
In
the
realm
of
biological
research,
invention
super-resolution
microscopy
(SRM)
has
enabled
visualization
ultrafine
sub-cellular
structures
and
their
functions
in
live
cells
at
nano-scale
level,
beyond
diffraction
limit,
which
opened
up
a
new
window
for
advanced
biomedical
studies
to
unravel
complex
unknown
details
physiological
disorders
level
with
unprecedented
resolution
clarity.
However,
most
SRM
techniques
are
highly
reliant
on
personalized
special
photophysical
features
fluorophores.
recent
times,
there
been
an
surge
development
robust
fluorophore
systems
various
imaging
techniques.
To
date,
xanthene,
cyanine,
oxazine
BODIPY
cores
have
authoritatively
utilized
as
basic
units
small-molecule-based
organic
fluorescent
probe
designing
strategies
owing
excellent
characteristics
easy
synthetic
acquiescence.
Since
future
next-generation
will
be
decided
by
availability
probes
these
four
building
blocks
play
important
role
progressive
design,
is
urgent
need
review
advancements
fluorophores
different
methods
based
dye
cores.
This
article
not
only
includes
comprehensive
discussion
about
developments
emphasis
effective
integration
into
cell
bio-imaging
applications
but
also
critically
evaluates
background
each
highlight
merits
demerits
towards
developing
newer
SRM.
Frontiers in Chemistry,
Journal Year:
2021,
Volume and Issue:
9
Published: June 24, 2021
Phototherapies,
in
the
form
of
photodynamic
therapy
(PDT)
and
photothermal
(PTT),
are
very
promising
treatment
modalities
for
cancer
since
they
provide
locality
turn-on
mechanism
toxicity,
both
which
critical
reducing
off-site
toxicity.
Irradiation
photosensitive
agents
demonstrated
successful
therapeutic
outcomes;
however,
each
approach
has
its
limitations
needs
to
be
improved
clinical
success.
The
combination
PTT
PDT
may
work
a
synergistic
way
overcome
method
indeed
improve
efficacy.
development
single
capable
inducing
is,
therefore,
extremely
advantageous
highly
desired.
Cyanine
dyes
shown
have
such
potential,
hence
been
popular
recent
years.
Luminescence
cyanine
renders
them
as
phototheranostic
molecules,
reporting
localization
agent
prior
irradiation
induce
phototoxicity,
allowing
image-guided
phototherapy.
In
this
review,
we
mainly
focus
on
dye–based
phototherapy
different
cells,
concentrating
advancements
achieved
last
ten
Journal of the American Chemical Society,
Journal Year:
2022,
Volume and Issue:
144(48), P. 21992 - 21999
Published: Nov. 22, 2022
Sulfoxide-bridged
dimeric
BODIPYs
were
developed
as
a
new
class
of
long-wavelength
photoconvertible
fluorophores.
Upon
visible-light
irradiation,
sulfoxide
moiety
was
released
to
generate
the
corresponding
α,α-directly
linked
BODIPYs.
The
extrusion
SO
from
sulfoxides
mainly
through
an
intramolecular
fashion
involving
reactive
triplet
states.
By
this
photoconversion,
not
only
more
than
100
nm
red
shifts
absorption
and
emission
maxima
(up
648/714
nm)
achieved
but
also
stable
products
with
bright
fluorescence
produced
high
efficiency.
combination
photoactivation
red-shifted
excitation/emission
offered
optimal
contrast
eliminated
interference
biological
autofluorescence.
More
importantly,
in
situ
these
visible-light-induced
reactions
demonstrated
ideal
single-molecule
properties
near-infrared
region.
Therefore,
photoconversion
could
be
powerful
method
achieving
super-resolution
localization
imaging
living
cell
without
using
UV
illumination
cell-toxic
additives.
Angewandte Chemie International Edition,
Journal Year:
2022,
Volume and Issue:
62(1)
Published: Sept. 30, 2022
Decoding
cellular
processes
requires
visualization
of
the
spatial
distribution
and
dynamic
interactions
biomolecules.
It
is
therefore
not
surprising
that
innovations
in
imaging
technologies
have
facilitated
advances
biomedical
research.
The
advent
super-resolution
has
empowered
researchers
with
ability
to
answer
long-standing
questions
about
at
an
entirely
new
level.
Fluorescent
probes
greatly
enhance
specificity
resolution
experiments.
Here,
we
introduce
key
technologies,
a
brief
discussion
on
single-molecule
localization
microscopy
(SMLM).
We
evaluate
chemistry
photochemical
mechanisms
fluorescent
employed
SMLM.
This
Review
provides
guidance
identification
adoption
single
molecule
inspire
design
next-generation
amenable
imaging.
Advanced Functional Materials,
Journal Year:
2023,
Volume and Issue:
33(38)
Published: May 26, 2023
Abstract
Organic
dyes
in
the
excited
singlet
state
(S
1
)
decay
via
intersystem
crossing
(ISC)
to
triplet
state,
radiative
dissipation
as
fluorescence,
or
nonradiative
for
thermal
deactivation.
Although
many
studies
are
conducted
improve
ISC
and
fluorescence
efficiency,
few
have
optimized
S
deactivation,
which
is
crucial
designing
photothermal
agents.
A
strategy
inhibiting
by
introducing
electron
withdraw
groups
(EWGs)
into
meso
position
of
heptamethine
cyanines
(Cy7)
reported
here,
allows
energy
relaxation.
The
decrease
density
Cy7
caused
EWGs
improved
photostability,
important
biological
applications
because
conventional
cyanine
easily
photobleached.
EWG
substitutes
acted
efficient
rotation
with
low‐energy
barriers,
narrowing
gap
between
ground
considerably
improving
conversion
efficiency
(PCE).
PCE
CF
3
cy
strongest
increased
up
83%.
Liposome
used
a
carrier
biocompatibility
tumor
retention
cy,
combined
toll‐like
receptor
agonist
resiquimod
(R848)
synergistic
immunotherapy
against
both
primary
distant
tumors
elicits
long‐lasting
immunological
memory
effect.
Journal of the American Chemical Society,
Journal Year:
2023,
Volume and Issue:
145(27), P. 14647 - 14659
Published: June 27, 2023
Heptamethine
indocyanines
are
invaluable
probes
for
near-infrared
(NIR)
imaging.
Despite
broad
use,
there
only
a
few
synthetic
methods
to
assemble
these
molecules,
and
each
has
significant
limitations.
Here,
we
report
the
use
of
pyridinium
benzoxazole
(PyBox)
salts
as
heptamethine
indocyanine
precursors.
This
method
is
high
yielding,
simple
implement,
provides
access
previously
unknown
chromophore
functionality.
We
applied
this
create
molecules
address
two
outstanding
objectives
in
NIR
fluorescence
First,
used
an
iterative
approach
develop
protein-targeted
tumor
When
compared
common
fluorophores,
optimized
probe
increases
specificity
monoclonal
antibody
(mAb)
nanobody
conjugates.
Second,
developed
cyclizing
with
goal
improving
cellular
uptake
fluorogenic
properties.
By
modifying
both
electrophilic
nucleophilic
components,
demonstrate
that
solvent
sensitivity
ring-open/ring-closed
equilibrium
can
be
modified
over
wide
range.
then
show
chloroalkane
derivative
compound
tuned
cyclization
properties
undergoes
particularly
efficient
no-wash
live
cell
imaging
using
organelle-targeted
HaloTag
self-labeling
proteins.
Overall,
chemistry
reported
here
broadens
scope
accessible
functionality,
and,
turn,
enables
discovery
promising
advanced
applications.
Journal of the American Chemical Society,
Journal Year:
2023,
Volume and Issue:
145(6), P. 3335 - 3345
Published: Feb. 6, 2023
Multicomponent
bioluminescence
imaging
in
vivo
requires
an
expanded
collection
of
tissue-penetrant
probes.
Toward
this
end,
we
generated
a
new
class
near-infrared
(NIR)
emitting
coumarin
luciferin
analogues
(CouLuc-3s).
The
scaffolds
were
easily
accessed
from
commercially
available
dyes.
Complementary
mutant
luciferases
for
the
CouLuc-3
also
identified.
brightest
probes
enabled
sensitive
vivo.
are
orthogonal
to
popular
bioluminescent
reporters
and
can
be
used
multicomponent
applications.
Collectively,
work
showcases
set
tools
that
readily
implemented
multiplexed
variety
biological
settings.
Smart Molecules,
Journal Year:
2024,
Volume and Issue:
2(3)
Published: Aug. 23, 2024
Abstract
In
situ
precise
detection
of
bioactive
molecules
with
high
sensitivity
and
spatiotemporal
resolution
is
essential
for
studying
physiological
events
disease
diagnosis.
The
utilization
versatile
fluorescent
probes
in
fluorescence
imaging
offers
a
powerful
tool
vivo
biomarkers
closely
associated
pathological
conditions.
However,
the
dynamic
behavior
leading
to
rapid
clearance
small
molecule
from
regions
interest
severely
compromises
their
potential
imaging.
Notably,
self‐immobilizing
that
selectively
recognize
diseased
tissues
while
improving
retention
enrichment
enable
accurate
high‐fidelity
this
review,
we
aim
summarize
strategies
employed
recent
advances
performance
precision
using
techniques.
Lastly,
discuss
prospects
challenges
promote
further
development
application
more
delicate
probes.
