The Journal of Physical Chemistry B,
Journal Year:
2023,
Volume and Issue:
127(14), P. 3208 - 3222
Published: April 3, 2023
Photoisomerization
kinetics
of
the
near-infrared
(NIR)
fluorophore
Sulfo-Cyanine7
(SCy7)
was
studied
by
a
combination
fluorescence
correlation
spectroscopy
(FCS)
and
transient
state
(TRAST)
excitation
modulation
spectroscopy.
A
photoisomerized
with
redshifted
emission
identified,
consistent
three-state
photoisomerization
model.
Combining
TRAST
spectrofluorimetry
(spectral-TRAST)
further
confirmed
an
excitation-induced
redshift
in
spectrum
SCy7.
We
show
how
this
red-emissive
contributes
to
blinking
different
bands
NIR
cyanine
dyes,
it
can
influence
single-molecule,
super-resolution,
as
well
Förster
resonance
energy
transfer
(FRET)
multicolor
readouts.
Since
also
be
populated
at
moderate
intensities,
more
broadly
readouts,
readouts
not
relying
on
high
conditions.
However,
additional
its
photodynamics,
identified
characterized
work,
used
strategy
push
dyes
into
enhance
photosensitization
nanoparticles
absorption
spectra
NIR.
Finally,
we
that
SCy7
formation
photoisomer
depend
strongly
local
environmental
conditions,
such
viscosity,
polarity,
steric
constraints,
which
suggests
use
other
sensors.
Such
information
monitored
TRAST,
NIR,
low
autofluorescence
scattering
conditions
broad
range
samples
experimental
Analytical Chemistry,
Journal Year:
2022,
Volume and Issue:
94(28), P. 10283 - 10290
Published: July 1, 2022
G-quadruplex
(G4)
is
a
noncanonical
nucleic
acid
secondary
structure
that
has
implications
for
various
physiological
and
pathological
processes
thus
essential
to
exploring
new
approaches
G4
detection
in
live
cells.
However,
the
deficiency
of
molecular
imaging
tools
makes
it
challenging
visualize
ex
vivo
tissue
samples.
In
this
study,
we
established
probe
design
strategy
presented
red
fluorescent
benzothiazole
derivative,
ThT-NA,
detect
image
structures
living
cells
By
enhancing
electron-donating
group
thioflavin
T
(ThT)
optimizing
structure,
ThT-NA
shows
excellent
photophysical
properties,
including
emission
(610
nm),
large
Stokes
shift
(>100
high
sensitivity
selectivity
toward
G4s
(1600-fold
fluorescence
turn-on
ratio)
robust
two-photon
emission.
Therefore,
these
features
enable
reveal
endogenous
RNA
distribution
differentiate
cell
cycle
by
monitoring
changes
folding.
Significantly,
best
our
knowledge,
first
benzothiazole-derived
been
developed
cancer
samples
microscopy
techniques.
Angewandte Chemie International Edition,
Journal Year:
2022,
Volume and Issue:
61(38)
Published: June 28, 2022
Abstract
Environment‐responsive
in
situ
synthesis
of
molecular
fluorescent
dyes
is
challenging.
Herein,
we
develop
a
photoextension
strategy
to
make
trimethine
cyanines
with
decent
conversion
efficiency
(up
81
%)
using
1‐butyl
2,3,3‐trimethyl
3
H
‐indole
derivatives
as
the
sole
precursors,
and
demonstrate
free
radical
mechanism.
In
inducer‐extension
stage,
radicals
reactive
oxygen
species
(ROS)
were
able
mediate
similar
reactions
no
assistance
light.
We
explored
Mito‐extension
synthesize
living
cells.
The
cellular
ROS‐dependence
provided
foundation
for
preferential
cyanine
expression
cancer
Finally,
applied
an
iodized
precursor
intrinsic
ROS‐activated
theranostic
agent
that
integrated
mitochondria‐targeted
synthesis,
cell
imaging
phototherapy.
EBioMedicine,
Journal Year:
2024,
Volume and Issue:
102, P. 105050 - 105050
Published: March 14, 2024
BackgroundNoninvasive
in
vivo
cell
tracking
is
valuable
understanding
the
mechanisms
that
enhance
anti-cancer
immunity.
We
have
recently
developed
a
new
method
called
phototruncation-assisted
(PACT),
uses
photoconvertible
technology
to
detect
migration.
This
has
advantages
of
not
requiring
genetic
engineering
cells
and
employing
tissue-penetrant
near-infrared
light.MethodsWe
applied
PACT
monitor
migration
immune
between
tumour
its
tumour-draining
lymph
node
(TDLN)
after
photoimmunotherapy
(NIR-PIT).FindingsPACT
showed
significant
increase
dendritic
(DCs)
macrophages
from
TDLN
immediately
NIR-PIT.
by
NIR-PIT
was
abrogated
inhibiting
sphingosine-1-phosphate
pathway
or
Gαi
signaling.
These
results
were
corroborated
intranodal
profiles
at
two
days
post-treatment;
significantly
induced
DC
maturation
increased
activated
CD8+
T
population
TDLN.
Furthermore,
revealed
enhanced
four
post-treatment,
which
consistent
with
immunohistochemical
assessment
tumour-infiltrating
lymphocytes
regression.InterpretationImmune
dramatically
migrated
following
NIR-PIT,
indicating
potential
as
an
immune-stimulating
therapy.
Also,
potentially
applicable
wide
range
immunological
research.FundingThis
work
supported
Intramural
Research
Program
National
Institutes
Health,
Cancer
Institute,
Centre
for
(grant
number:
ZIA
BC011513
BC011506).
Nanomaterials,
Journal Year:
2024,
Volume and Issue:
14(14), P. 1215 - 1215
Published: July 17, 2024
Reliable
cell
labeling
and
tracking
techniques
are
imperative
for
elucidating
the
intricate
ambiguous
interactions
between
mesenchymal
stromal
cells
(MSCs)
tumors.
Here,
we
explore
fluorescent
photoconvertible
nanoengineered
vesicles
to
study
mMSC
migration
in
brain
These
3
μm
sized
made
of
carbon
nanoparticles,
Rhodamine
B
(RhB),
polyelectrolytes
readily
internalized
by
cells.
The
dye
undergoes
photoconversion
under
561
nm
laser
exposure
with
a
fluorescence
blue
shift
upon
demand.
optimal
irradiation
duration
was
0.4
ms,
which
provided
maximal
signal
label
without
excessive
on
Vesicles
modified
an
extra
polymer
layer
demonstrated
enhanced
intracellular
uptake
remarkable
effects
viability,
motility,
or
proliferation.
ratio
20
per
determined.
Moreover,
individual
mMSCs
within
2D
3D
glioblastoma
(EPNT-5)
colonies
over
2
days
vivo
tumor
settings
7
were
traced.
Our
provides
robust
nanocomposite
platform
investigating
MSC–tumor
dynamics
offers
insights
into
envisaged
therapeutic
strategies.
Photoconvertible
also
present
indispensable
tool
studying
complex
fundamental
processes
cell–cell
wide
range
problems
biomedicine.
The Journal of Physical Chemistry B,
Journal Year:
2023,
Volume and Issue:
127(14), P. 3208 - 3222
Published: April 3, 2023
Photoisomerization
kinetics
of
the
near-infrared
(NIR)
fluorophore
Sulfo-Cyanine7
(SCy7)
was
studied
by
a
combination
fluorescence
correlation
spectroscopy
(FCS)
and
transient
state
(TRAST)
excitation
modulation
spectroscopy.
A
photoisomerized
with
redshifted
emission
identified,
consistent
three-state
photoisomerization
model.
Combining
TRAST
spectrofluorimetry
(spectral-TRAST)
further
confirmed
an
excitation-induced
redshift
in
spectrum
SCy7.
We
show
how
this
red-emissive
contributes
to
blinking
different
bands
NIR
cyanine
dyes,
it
can
influence
single-molecule,
super-resolution,
as
well
Förster
resonance
energy
transfer
(FRET)
multicolor
readouts.
Since
also
be
populated
at
moderate
intensities,
more
broadly
readouts,
readouts
not
relying
on
high
conditions.
However,
additional
its
photodynamics,
identified
characterized
work,
used
strategy
push
dyes
into
enhance
photosensitization
nanoparticles
absorption
spectra
NIR.
Finally,
we
that
SCy7
formation
photoisomer
depend
strongly
local
environmental
conditions,
such
viscosity,
polarity,
steric
constraints,
which
suggests
use
other
sensors.
Such
information
monitored
TRAST,
NIR,
low
autofluorescence
scattering
conditions
broad
range
samples
experimental
