Exchange of free and capsule conjugated cyanine dyes between cells DOI
Zhanna V. Kozyreva, Polina A. Demina, Оlga I. Gusliakova

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Fluorescent dyes (especially photoconvertible cyanine dyes) are traditionally used as labels to study single-cell or cell-group interactions and migration. Nevertheless, their application has some disadvantages, such cytotoxicity dye transfer between cells during co-cultivation. The latter can lead serious distortions in research results. At the same time, lack of a worthy alternative explains reasons for hushing up this problem. Here, we propose low-cytotoxicity encapsulated forms 3.5 5.5, enabling intracellular uptake facilitating labeling tracking an efficient existing staining. Only 16.9% myoblasts (C2C12) exchanged compared with 99.7% that free form dyes. Simultaneous several dyes, combined possibility photoconversion, provides multi-color coding individual cells. Encapsulation allows reliable reduces

Language: Английский

The pursuit of linear dosage in pharmacy: reservoir-based drug delivery systems from macro to micro scale DOI

Arkady S. Abdurashtov,

Pavel I. Proshin, Gleb B. Sukhorukov

et al.

Expert Opinion on Drug Delivery, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 20

Published: Jan. 7, 2025

Introduction The pursuit of linear dosage in pharmacy is essential for achieving consistent therapeutic release and enhancing patient compliance. This review provides a comprehensive summary zero-order drug delivery systems, with particular focus on reservoir-based systems emanated from different microfabrication technologies.

Language: Английский

Citations

1

Brain neurons internalise polymeric micron-sized capsules: insights from in vitro and in vivo studies DOI Creative Commons
Olga Kopach, Olga A. Sindeeva, Kaiyu Zheng

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101493 - 101493

Published: Jan. 22, 2025

Nanoengineered encapsulation presents a promising strategy for targeted drug delivery to specific regions in the body. While polyelectrolyte-based biodegradable microcapsules can achieve highly localised release tissues and cell cultures, delivering drugs intracellular sites brain remains significant challenge. In this study, we utilized advanced imaging techniques, both vitro vivo, investigate whether neurons internalise designed delivery. High-resolution live-cell revealed that differentiating N2A cells actively microcapsules, often incorporating multiple capsules per cell. Likewise, primary hippocampal cortical were observed effectively polymeric microcapsules. intact brain, multiplexed two-photon excitation vivo confirmed internalisation of by following somatosensory region. This was time-dependent, correlated with particle size mediated macropinocytosis mechanism appears bypass lysosomal formation. Importantly, presence internalised did not impair neuronal function, as maintained normal firing activity action potential characteristics. Furthermore, no adverse effects after week microcapsule mouse brain. Our findings indicate are effective safe carriers neurons, providing approach therapeutic applications.

Language: Английский

Citations

0

Engineering colloidal systems for cell manipulation, delivery, and tracking DOI
Olga A. Sindeeva, Zhanna V. Kozyreva, Arkady S. Abdurashitov

et al.

Advances in Colloid and Interface Science, Journal Year: 2025, Volume and Issue: 340, P. 103462 - 103462

Published: Feb. 27, 2025

Language: Английский

Citations

0

Photoconvertible markers for study individual myoblast migration into the macrophage’s colony DOI
Polina A. Demina, Оlga I. Gusliakova, Zhanna V. Kozyreva

et al.

Optical Materials, Journal Year: 2024, Volume and Issue: unknown, P. 116148 - 116148

Published: Sept. 1, 2024

Language: Английский

Citations

1

Exchange of free and capsule conjugated cyanine dyes between cells DOI
Zhanna V. Kozyreva, Polina A. Demina, Оlga I. Gusliakova

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Fluorescent dyes (especially photoconvertible cyanine dyes) are traditionally used as labels to study single-cell or cell-group interactions and migration. Nevertheless, their application has some disadvantages, such cytotoxicity dye transfer between cells during co-cultivation. The latter can lead serious distortions in research results. At the same time, lack of a worthy alternative explains reasons for hushing up this problem. Here, we propose low-cytotoxicity encapsulated forms 3.5 5.5, enabling intracellular uptake facilitating labeling tracking an efficient existing staining. Only 16.9% myoblasts (C2C12) exchanged compared with 99.7% that free form dyes. Simultaneous several dyes, combined possibility photoconversion, provides multi-color coding individual cells. Encapsulation allows reliable reduces

Language: Английский

Citations

1