Adverse Outcomes Following Exposure to Perfluorooctanesulfonamide (PFOSA) in Larval Zebrafish (Danio rerio): A Neurotoxic and Behavioral Perspective DOI Creative Commons

Nikita David,

Emma Ivantsova, Isaac Filipe Moreira Konig

et al.

Toxics, Journal Year: 2024, Volume and Issue: 12(10), P. 723 - 723

Published: Oct. 4, 2024

Toxicity mechanisms of per- and polyfluoroalkyl substances (PFASs), a chemical class present in diverse ecosystems, as well many their precursors, have been increasingly characterized aquatic species. Perfluorooctanesulfonamide (PFOSA, C8H2F17NO2S) is common precursor perfluorooctane sulfonic acid (PFOS), long-chain PFAS. Here, we assessed sub-lethal endpoints related to development, oxidative stress, transcript levels, distance moved zebrafish embryos larvae following continuous exposure PFOSA beginning at 6 h post-fertilization (hpf). decreased survival fish treated with 1 µg/L PFOSA; however, the effect was modest relative controls (difference 10%). Exposure up 10 did not affect hatch rate, nor it induce ROS 7-day-old fish. The activity larval 100 reduced solvent control. Transcripts stress response apoptosis were measured BCL2-associated X, regulator (bax), cytochrome c, somatic (cycs), catalase (cat), superoxide dismutase 2 (sod2) induced high concentrations PFOSA. Genes neurotoxicity also levels acetylcholinesterase (ache), elav-like RNA binding protein 3 (elavl3), growth-associated 43 (gap43), synapsin II (syn2a), tubulin (tubb3) all increased higher exposure. These data improve our understanding potential toxicity

Language: Английский

Combined toxic effects of polystyrene microplastics and 3,6-dibromocarbazole on zebrafish (Danio rerio) embryos DOI
Jie Zhang,

Yao Bai,

Haoran Meng

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 913, P. 169787 - 169787

Published: Jan. 4, 2024

Language: Английский

Citations

14
Emerging disinfection byproducts 3-bromine carbazole induces cardiac developmental toxicity via aryl hydrocarbon receptor activation in zebrafish larvae DOI
Yingying Liu,

Xudong Jin,

Yanan Ye

et al.

Environmental Pollution, Journal Year: 2024, Volume and Issue: 346, P. 123609 - 123609

Published: Feb. 23, 2024

Language: Английский

Citations

11
Spatial distribution characteristics of carbazole and polyhalogenated carbazoles in water column and sediments in the open Western Pacific Ocean DOI

Songtao Hu,

Lijia Jiang,

Lingbo Jiang

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 469, P. 133956 - 133956

Published: March 6, 2024

Language: Английский

Citations

9
Dihalogenated nitrophenols in drinking water: Prevalence, resistance to household treatment, and cardiotoxic impact on zebrafish embryo DOI Creative Commons
Hongjie Sun, Yingying Liu, Chunxiu Wu

et al.

Eco-Environment & Health, Journal Year: 2024, Volume and Issue: 3(2), P. 183 - 191

Published: March 4, 2024

Dihalogenated nitrophenols (2,6-DHNPs), an emerging group of aromatic disinfection byproducts (DBPs) detected in drinking water, have limited available information regarding their persistence and toxicological risks. The present study found that 2,6-DHNPs are resistant to major water treatment processes (sedimentation filtration) households methods (boiling, filtration, microwave irradiation, ultrasonic cleaning). To further assess health risks, we conducted a series toxicology studies using zebrafish embryos as the model organism. Our findings reveal these showed lethal toxicity 248 times greater than regulated DBP, dichloroacetic acid. Specifically, at sublethal concentrations, exposure generated reactive oxygen species (ROS), caused apoptosis, inhibited cardiac looping, induced failure zebrafish. Remarkably, use ROS scavenger, N-acetyl-L-cysteine, considerably mitigated adverse effects, emphasizing ROS' essential role 2,6-DHNP-induced cardiotoxicity. highlight cardiotoxic potential even low concentrations 19 μg/L beneficial effect N-acetyl-L-cysteine alleviating This underscores urgent need for increased scrutiny compounds public discussions.

Language: Английский

Citations

7
Polyhalogenated carbazoles in the environment: Analysis, origins, occurrence, and toxicity DOI

Jiamin Tu,

Xiangying Zeng, Liang Yi

et al.

Environmental Pollution, Journal Year: 2025, Volume and Issue: unknown, P. 125910 - 125910

Published: Feb. 1, 2025

Language: Английский

Citations

0
Swim in Plastics: Clean Nanoplastics Cause Minimal Mortality but Alter Neurobehavioral and Molecular Rhythms in Fish DOI
Xiaoyu Duan, Mohamed Helal, Xin Wang

et al.

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Nanoplastics (NPs) pose potential ecological and health impacts. While previous studies have highlighted inconsistent toxicity levels of NPs, knowledge remains limited about the specific effects different NPs on embryonic development, early life-stage behaviors, bodily uptake. This study examines polystyrene (PS-NPs) with surface charges, plain (PS), amino-modified (PS-NH2), carboxyl-modified (PS-COOH) zebrafish life stages. High-resolution 3D bioimaging confirmed differential internalization: PS-COOH accumulated in yolk intestine, while PS-NH2 localized mainly intestine. PS-NPs up to 10 ppm did not significantly affect mortality or hatching rates, likely due effective dialysis, minimizing toxic chemical leaching. PS-NP exposure led noninflated swim bladders affected swimming. RNA sequencing identified impacted neurological molecular pathways like circadian rhythm visual function; weighted gene coexpression network analysis indicated strong correlations between key modules phenotypic traits eye development dopamine level. We highlight low acute clean dialyzed despite uptake surface-charge-dependent sublethal neurotoxicity. Overall, induced significant individual-level effects, charged caused stronger molecular-level alterations; profiles varied across biological levels, complicating hazard assessment underscoring need for population-level

Language: Английский

Citations

0
Dihalogenated nitrophenols exposure induces developmental neurotoxicity in zebrafish embryo DOI Creative Commons
Wenting Zhu, Yingying Liu,

Xiongzhi Xuan

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 277, P. 116359 - 116359

Published: April 24, 2024

2,6-Dihalogenated nitrophenols (2,6-DHNPs) are emerging halogenated nitroaromatic pollutants that have been detected in various water environments. However, there is currently limited research available regarding their potential impacts on locomotion behavior and neurotoxicity. Therefore, this study utilized zebrafish embryos to investigate the neurotoxic effects of 2,6-DHNPs by examining impact nervous system at a concentration defined as 10% median lethal concentration. Our findings demonstrated exposure resulted significant 30 % decrease total swimming distance larvae, accompanied notable impairments motor neuron development central system. These were evidenced substantial 25% axonal growth, well disruptions synapse formation neuronal differentiation. Additionally, neurotransmitter analysis revealed marked decreases 40%, 35%, 30% dopamine, 5-hydroxytryptamine, acetylcholine levels respectively, highlighting disturbances synthesis, transport, degradation mechanisms. results emphasize considerable neurotoxicity concentrations previously considered safe; thus necessitating re-evaluation environmental risk assessments regulatory standards for such contaminants.

Language: Английский

Citations

3
Comparing the Developmental Toxicity Delay and Neurotoxicity of Benzothiazole and Its Derivatives (BTHs) in Juvenile Zebrafish DOI Creative Commons

Xiaogang Yin,

Lei Wang,

Mao Lian-shan

et al.

Toxics, Journal Year: 2024, Volume and Issue: 12(5), P. 341 - 341

Published: May 7, 2024

In this study, a semi-static water exposure method was employed to investigate the early developmental and neurotoxic effects of four benzothiazole substances (BTHs), namely (BTH), 2-mercaptobenzothiazole (MBT), 2-hydroxybenzothiazole (BTON), 2-aminobenzothiazole (2-ABTH), on zebrafish at an equimolar concentration 10 μM. The findings revealed that all BTHs exerted certain impacts development in zebrafish. MBT stimulated spontaneous movement juvenile zebrafish, whereas BTON inhibited such movements. Moreover, hindered hatching process larvae, with exhibiting strongest inhibition 24 h post-fertilization (hpf). Notably, acted as melanin inhibitor by suppressing production eyes weakening phototaxis. Additionally, both BTH exhibited significantly lower speeds than control group other test groups under conditions without bright field stimulation; however, their increased average levels after percussion stimulation, indicating no significant alteration motor ability among experimental groups. Short-term these types induced oxidative damage larvae; specifically, BTH-, MBT-, BTON-exposed displayed abnormal expression patterns genes related damage. Exposure led reduced fluorescence intensity transgenic labeled central nervous system markers, suggesting development. Furthermore, real-time quantitative PCR results demonstrated gene associated neural However, changes were observed 2-ABTH concentration. Overall indicate short-term stimulates neurodevelopmental accompanied

Language: Английский

Citations

3
Natural pyrethrins induce cytotoxicity in SH-SY5Y cells and neurotoxicity in zebrafish embryos (Danio rerio) DOI
Weiguo Wang, Shoulin Li,

Bin Liu

et al.

Environmental Research, Journal Year: 2024, Volume and Issue: 257, P. 119267 - 119267

Published: May 28, 2024

Language: Английский

Citations

2
Induction of oxidative stress and cardiac developmental toxicity in zebrafish embryos by arsenate at environmentally relevant concentrations DOI Creative Commons
Wenqian Wang,

Yanan Ye,

Yingying Liu

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 280, P. 116529 - 116529

Published: June 5, 2024

The contamination of water by arsenic (As) has emerged as a significant environmental concern due to its well-documented toxicity. Environmentally relevant concentrations As have been reported pose considerable threat fish. However, previous studies mainly focused on the impacts at environmentally adult fish, and limited information is available regarding fish early life stage. In this study, zebrafish embryos were employed evaluate risks following exposure different (0, 25, 50, 75 150 μg/L) pentavalent arsenate (AsV) for 120 hours post fertilization. Our findings indicated that ≤ μg/L AsV did not exert effects survival or aberration; however, it conspicuously inhibited heart rate larvae. Furthermore, significantly disrupted mRNA transcription genes associated with cardiac development, elongated distance between sinus venosus bulbus arteriosus treatments. Additionally, enhanced superoxide dismutase (SOD) activity treatments, increased transcriptional levels Cu/ZnSOD MnSOD Concurrently, suppressed metallothionein1 (MT1) MT2 transcriptions while elevating heat shock protein70 in larvae resulting elevated malondialdehyde (MDA) levels. These provide novel insights into toxic exerted low stage, thereby contributing an exploration concentrations.

Language: Английский

Citations

2