Pharmacokinetic Analysis of Gatifloxacin and Dexamethasone in Rabbit Ocular Biofluid Using a Sensitive and Selective LC–MS/MS Method DOI

Arpon Biswas,

Abhijit Deb Choudhury,

Anjali Mishra

et al.

Journal of Mass Spectrometry, Journal Year: 2024, Volume and Issue: 59(10)

Published: Oct. 1, 2024

ABSTRACT Bacterial keratitis (BK) is an infection that causes inflammation of the cornea and, if severe, can result in blindness. Topical fluoroquinolones combined with corticosteroids have been shown to be useful treatment BK. A rapid, selective, and sensitive bioanalytical method for simultaneous quantification Gatifloxacin (GAT) Dexamethasone (DEX) has developed validated using tandem mass spectrometry (LC–MS/MS). Optimal separation was accomplished under 5 min Agilent Zorbax C18 column (100 mm × 4.6 mm, 3.5 μm). The mobile phase composed a blend 0.2% formic acid triple distilled water methanol flow rate 0.65 mL/min isocratic mode. GAT DEX were detected positive electrospray ionization multiple reaction monitoring mode (MRM), retention time found at 1.64 2.93 min, respectively. linearity range 1.56–400 ng mL −1 good precision accuracy. according USFDA regulatory guidelines. effectively utilized preclinical pharmacokinetic analysis rabbit tear fluid following topical application commercial formulation.

Language: Английский

Fungal infection monitoring on corneal epithelium ex vivo model and its collection over polyethersulfone membrane for detecting Candida albicans and Aspergillus fumigatus DOI Creative Commons

Sarp Orgul,

Ángela Bedoya, Víctor Pérez

et al.

Medical Microbiology and Immunology, Journal Year: 2025, Volume and Issue: 214(1)

Published: Feb. 7, 2025

In this paper we report a human ex vivo model of Aspergillus fumigatus and Candida albicans infection the cornea. We demonstrate ability to monitor fungal growth on cornea through optical immunofluorescence microscopy. Additionally, establish method for collection antigens in polyethersulfone (PES) membrane-based sample collectors from surface inoculated corneas. Immunofluorescence microscopy was performed directly both corneal tissue PES membrane collectors. For latter case, devised an original ad-hoc by attaching membranes used standard glass slides, which call slide KIT. This enabled easy handling improved efficiency specifically recognizing corresponding infections simple immunoassay protocols. As result, firstly observe fungi tissue, secondly adsorption onto membranes. Thus, first time specific detection use based KITs immunofluorescence, as alternative microbiological culture.

Language: Английский

Citations

1
Macrophomina phaseolina: A Phytopathogen Associated with Human Ocular Infections—A Case Report of Endophthalmitis and Systematic Review of Human Infections DOI Open Access
Panagiotis Toumasis, Georgia Vrioni,

Ioanna Gardeli

et al.

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(2), P. 430 - 430

Published: Jan. 11, 2025

Background: Macrophomina phaseolina is an important phytopathogenic fungus affecting over 500 plant species worldwide. However, this rarely causes disease in humans. Methods: We reported the first case of endophthalmitis due to M. phaseolina, describing microbiological diagnostic approaches. Also, we performed a systematic review human infections by pathogen literature. searched PubMed, Scopus, and Web Science databases from inception 31 December 2024. Results: Our involved male patient who presented with photophobia pain his right eye. His recent medical history revealed superficial corneal injury caused metal burr three months prior, managed unsuccessfully topical treatment subsequent conjunctival flap surgery two later. Ophthalmological investigations, including microscopic examination, cultures, DNA sequencing ocular specimens, endophthalmitis. Despite intravenous intravitreal antifungal therapy, patient’s condition continued worsen, eventually leading enucleation. Regarding literature review, identified 12 additional cases previously Overall, was primarily associated (76.9% cases), followed skin combined skin–joint infections. The majority patients infection (63.6%) had no known immunosuppressive factors. Clinical outcomes were unfavorable 46.15% cases. Conclusions: emerging cause infections, even immunocompetent hosts, predilection for Further research warranted elucidate pathogenesis fungal pathogens

Language: Английский

Citations

0
A Novel HPLC Method for Simultaneous Estimation of Natamycin and Voriconazole for Ocular Drug Delivery Applications DOI

Velmurugan Kailasam,

Oindrilla Dasgupta,

Tamizhmathy Mannangatty

et al.

Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2025, Volume and Issue: unknown, P. 116705 - 116705

Published: Jan. 1, 2025

Language: Английский

Citations

0
Inhibition of LRRK2 Ameliorates Aspergillus fumigatus Keratitis by Regulating STING Signaling Pathways DOI Creative Commons
Fang Han, Leyi Wang, Jiayin Wu

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(2), P. 13 - 13

Published: Feb. 5, 2025

Purpose: The purpose of this study was to investigate the role LRRK2 in inflammatory response fungal keratitis (FK) and elucidate underlying mechanisms. Methods: protein levels leucine-rich repeat kinase 2 (LRRK2), p-LRRK2, stimulator interferon genes (STING)-related proteins were assessed by western blot analysis. ELISA quantitative real-time polymerase chain reaction (qRT-PCR) employed evaluate induced Aspergillus fumigatus. Mass spectrometry performed identify interaction partners LRRK2. glutathione S-transferase (GST) pull-down assay co-immunoprecipitation (co-IP) used verify between STING. Additionally, load determinations clinical score assessments conducted determine corneal infection a mouse model. Results: A. fumigatus stimulation promoted phosphorylation through Toll-like receptor (TLR2) human epithelial cells (HCECs) corneas. overexpression enhanced fumigatus–induced response, knockdown alleviated both vitro vivo. identified STING as novel partner Moreover, treatment STING, resulting activation phosphorylated then triggered its downstream signaling pathways, exacerbating severity keratitis. inhibitor (LRRK2-IN-1) significantly mitigated damage caused stimulation. Conclusions: inhibition ameliorates inflammation modulating pathways HCECs models. Our results suggest that targeted could be promising strategy for FK treatment.

Language: Английский

Citations

0
Seeing the unseen: The low treatment rate of eye emergencies in Africa DOI
Babatunde Ismail Bale, Marco Zeppieri, Obehi Suzan Idogen

et al.

World Journal of Methodology, Journal Year: 2025, Volume and Issue: 15(3)

Published: March 6, 2025

BACKGROUND Emergency medical care is essential in preventing morbidity and mortality, especially when interventions are time-sensitive require immediate access to supplies trained personnel. AIM To assess the treatment rates of eye emergencies Africa. Ocular particularly delicate due eye’s intricate structure necessity for its refractive components remain transparent. METHODS This review examines low Africa, drawing on 96 records extracted from PubMed database using predetermined search criteria. RESULTS The epidemiology ocular injuries, as detailed studies, reveals significant relationships between incidence prevalence injuries factors such age, gender, occupation. causes range accidents gender-based violence insect or animal attacks. Management approaches reported include both surgical non-surgical interventions, medication evisceration enucleation eye. Preventive measures emphasize health education use protective eyewear facial protection. However, inadequate healthcare infrastructure personnel, cultural geographical barriers, socioeconomic behavioral hinder effective prevention, service uptake, management emergencies. CONCLUSION authors recommend developing policies, enhancing community engagement, improving personnel training retention, increasing funding programs solutions address rate

Language: Английский

Citations

0
Antifungal susceptibility and clinical efficacy of chlorhexidine combined with topical ophthalmic medications against Fusarium species isolated from corneal samples DOI Creative Commons
Min Ho Kang, Yuxuan Wu,

Qingquan Shi

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: April 15, 2025

Objective This study investigated the susceptibility of various Fusarium fungi to five topical antifungal agents: natamycin, voriconazole, chlorhexidine, natamycin combined with and voriconazole chlorhexidine. And explore clinical feasibility combination therapy in treatment corneal infections caused by F. solani , goal optimizing regimen for fungal keratitis. Methods A total 194 strains were isolated from corneas between 2013 2024 identified species level using mass spectrometry. The MICs was determined a commercial microdilution plate assess vitro activity drugs used alone (natamycin/chlorhexidine, voriconazole/chlorhexidine). Additionally, efficacy prospectively observed 5 patients . regimens included 5% 0.04% chlorhexidine alone, or follow-up lasting up 90 days. Results complex (FSSC, 46.91%) fujikuroi (FFSC, 45.88%) predominant isolates, geographical distribution concentrated Northern China. FSSC FFSC both 2–8 μg /mL, respectively. respectively ranged 0.25–16 1–8 μg/mL 2 > 16 1 not significantly different FFSC. However, had higher compared Compared exhibited enhancement against 100% tested strains. 81.4% all spp., lower (65.9%) than non- (93.6%). Among patients, 3 received resulting cure (in 1-1.5 months), while patient required transplant due delayed treatment. One treated one achieved 2-3 months). Conclusion Natamycin spp. during sensitivity tests. findings this provide valuable guidance establishing epidemiological cutoff MIC values paves way future multicenter studies on FK

Language: Английский

Citations

0
Pathology-inspired collagen-binding thermosensitive micelle drops enable prolonged and efficient treatment of fungal keratitis DOI
Maoyu Cai,

Haiping Zhong,

Xindi Wang

et al.

Bioactive Materials, Journal Year: 2025, Volume and Issue: 50, P. 396 - 413

Published: April 22, 2025

Language: Английский

Citations

0
Itraconazole Loaded Micelle Based on Methoxy Poly(Ethylene Glycol)-Poly(D, L-Lactic Acid) for Ocular Drug Delivery: In vitro and in vivo Evaluation DOI Creative Commons

Jijun He,

Jingjing Yang, Zhen Liang

et al.

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 6447 - 6462

Published: May 1, 2025

This study aimed to develop itraconazole (ITZ)-loaded polymer micelles using methoxy poly(ethylene glycol)-poly(D, L-lactic acid) (mPEG-PDLLA) as a carrier improve the ocular bioavailability of ITZ after topical administration. ITZ-loaded mPEG-PDLLA (ITZ-M) were prepared thin-film dispersion method and characterized by droplet size (DS), zeta potential (ZP), polydispersity index (PDI), morphology, entrapment efficiency (EE%), critical micelle concentration (CMC). In vitro drug release from ITZ-M, storage stability cytotoxicity in human corneal epithelial cells (HCECs) studied. vivo transcorneal permeation labeled with coumarin 6 (C6) was observed two-photon confocal microscopy, irritation pharmacokinetics rabbit eyes investigated. The ITZ-Ms uniform spherical particles DS 18.79 ± 0.16 nm narrow distribution (PDI 0.037 0.019), EE% nearly 100%, CMC 0.083mM. Approximately 60% released ITZ-M within 72 h, which significantly higher than that suspension. results assays demonstrated possessed good physical at 4°C have no toxicity HCECs. Transcorneal studies indicated fluorescence intensity (FI) mostly enriched epithelium, reduced stroma. FI epithelium stroma for C6 much stronger Ocular evaluation revealed well tolerated. pharmacokinetic analysis area under curve (AUC0-240min) values cornea conjunctiva treated approximately 410.9- 2.3-fold higher, respectively, those provides formulation treatment fungal keratitis tolerability improved bioavailability.

Language: Английский

Citations

0
Pharmacokinetic Analysis of Gatifloxacin and Dexamethasone in Rabbit Ocular Biofluid Using a Sensitive and Selective LC–MS/MS Method DOI

Arpon Biswas,

Abhijit Deb Choudhury,

Anjali Mishra

et al.

Journal of Mass Spectrometry, Journal Year: 2024, Volume and Issue: 59(10)

Published: Oct. 1, 2024

ABSTRACT Bacterial keratitis (BK) is an infection that causes inflammation of the cornea and, if severe, can result in blindness. Topical fluoroquinolones combined with corticosteroids have been shown to be useful treatment BK. A rapid, selective, and sensitive bioanalytical method for simultaneous quantification Gatifloxacin (GAT) Dexamethasone (DEX) has developed validated using tandem mass spectrometry (LC–MS/MS). Optimal separation was accomplished under 5 min Agilent Zorbax C18 column (100 mm × 4.6 mm, 3.5 μm). The mobile phase composed a blend 0.2% formic acid triple distilled water methanol flow rate 0.65 mL/min isocratic mode. GAT DEX were detected positive electrospray ionization multiple reaction monitoring mode (MRM), retention time found at 1.64 2.93 min, respectively. linearity range 1.56–400 ng mL −1 good precision accuracy. according USFDA regulatory guidelines. effectively utilized preclinical pharmacokinetic analysis rabbit tear fluid following topical application commercial formulation.

Language: Английский

Citations

2