Polymer Solutions in Microflows: Tracking and Control over Size Distribution DOI Open Access
Artem Bezrukov, Yu. G. Galyametdinov

Polymers, Journal Year: 2024, Volume and Issue: 17(1), P. 28 - 28

Published: Dec. 26, 2024

Microfluidics provides cutting-edge technological advancements for the in-channel manipulation and analysis of dissolved macromolecular species. The intrinsic potential microfluidic devices to control key characteristics polymer macromolecules such as their size distribution requires unleashing its full capacity. This work proposes a combined approach analyzing microscale behavior solutions modifying properties. We utilized idea modeling cross-channel diffusion in polydisperse microflows using dynamic light scattering curves source data. model was implemented into Matlab script which predicts changes at chip outputs. verified predictions experiments with series microchips by detecting optical responses injected nematic liquid crystals presence species after processing. results offer new approaches tuning dispersity solution, developing auxiliary tools techniques scattering, labs-on-chips diagnostics processing polymers.

Language: Английский

Ferguson Plot Analysis of Chaperone ClpB from Moderate Halophile DOI
Teruo Akuta,

Yui Tomioka,

Tomoto Ura

et al.

The Protein Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 5, 2025

Language: Английский

Citations

0
Comparative Study of Single and Coaxial Electrospun Antimicrobial Cross-Linked Scaffolds Enriched with Aloe Vera: Characterization, Antimicrobial Activity, Drug Delivery, Cytotoxicity, and Cell Proliferation on Adipose Stem Cells and Human Skin Fibroblast DOI Creative Commons

Damian Francisco Plascencia Martínez,

Jesús Manuel Quiroz‐Castillo,

Alida Ospina Orejarena

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(40), P. 41157 - 41170

Published: Sept. 29, 2024

The preparation of materials with application in the biomedical field needs to attend some characteristics such as biocompatibility, nontoxicity, adequate mechanical properties, and ability mimic extracellular matrix. Scaffolds for use cell culture were prepared based on gelatin, polylactic acid (PLA), aloe vera mucilage, tetracycline. Fibers single coaxial configuration then cross-linked glutaraldehyde saturated vapor. fibers obtained cylindric morphology changed ribbon porous membranes, similarly matrix, when cross-linked. Membranes by electrospinning showed core-shell structures observed transversal images, which is beneficial controlled drug release. Characterization techniques scanning electron microscopy, thermogravimetric analysis, Fourier transform infrared spectroscopy demonstrated cross-linking due increase diameter, formation imine groups, improvement thermal stability. Antibiotic release tests that prevalent mechanism diffusion can be considering encapsulation effect, are a configuration, increasing time, making it suitable material biological evaluation scaffolds was carried out two lines: mammalian adipose stem cells (ASCs), used primary culture, Detroit 548 human skin fibroblasts dermal model. Aloe enriched better activity contact both lines, exhibiting viability values greater than 90% favorable results live-dead assays no damaged observed. Cell proliferation evaluated using fibroblast gelatin-based staining adhered cells; images good confluence antibiotic loaded membranes studied configurations. presented antimicrobial against

Language: Английский

Citations

2
Effect of Bidispersity on Polymer-Bound Layers of Carbon Black Primary Particles: Combining Large-Scale Simulations and Experiments DOI Creative Commons
Stefano Caputo,

Velichko Hristov,

Gianmarco Munaò

et al.

Macromolecules, Journal Year: 2023, Volume and Issue: 56(24), P. 10119 - 10131

Published: Dec. 4, 2023

In this work, we propose a combined computational and experimental study to investigate the effect of bidispersity polyethylene (PE) chains on interface interphase between polymer carbon black (CB) filler particles. To reach goal, have implemented molecular description based hybrid particle–field modeling approach that allows perform large-scale simulations CB primary particles realistic size (20 nm) embedded in bidisperse PE melts high weight simulated as models up about two million coarse-grained units (beads). Due detail proposed models, are able provide an accurate representation local structure adsorbed different surface sites Experiments performed with Borealis technology Borstar synthesize bimodal PE, together melts, possible mechanism involving short explain improved dispersion melts. The achieved insights can help design new optimized ternary mixtures.

Language: Английский

Citations

6
Self-organization of PIP3 waves is controlled by the topology and curvature of cell membranes DOI Creative Commons

Sema Erisis,

Marcel Hörning

Biophysical Journal, Journal Year: 2024, Volume and Issue: 123(9), P. 1058 - 1068

Published: March 22, 2024

Language: Английский

Citations

1
Self-organization of PIP3 signaling is controlled by the confinement, topology, and curvature of the cell membrane DOI Open Access

Sema Erisis,

Marcel Hörning

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 23, 2024

PIP3 is a signaling lipid on the plasma membrane that plays fundamental role in cell with strong impact physiology and diseases. It responsible for protruding edge formation, polarization, macropinocytosis other remodeling dynamics cells. has been shown confinement curvature affects wave formation of Factin. But even absence F-actin, complex self-organization spatiotemporal waves observed. In recent findings, we have these can be guided pinned strongly bended Dictyostelium membranes caused by molecular crowding limited diffusion. Based experimental investigate realist 3D cell-like to explore effect diffusion, as observed experimentally. We use an established stochastic reaction-diffusion model enzymatic MichaelisMenten type reactions mimics As cells mimic shape size experimentally, found directionality explained Hopf-like reverse periodic-doubling bifurcation uniform diffusion properties. Finally, compare results findings discuss discrepancy between biological numerical results.

Language: Английский

Citations

0
Ferguson Plot Analysis of Chaperone Clpb From Moderate Halophile DOI
Teruo Akuta,

Yui Tomioka,

Masataka Nakagawa

et al.

Published: Jan. 1, 2024

Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI

Language: Английский

Citations

0
Ferguson Plot Analysis of Chaperone Clpb From Moderate Halophile DOI
Teruo Akuta,

Yui Tomioka,

Tomoto Ura

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Coalescence of liquid or gel-like DNA-encapsulating microdroplets DOI
Takashi Nishio, Helmut Schiessel

The Journal of Chemical Physics, Journal Year: 2024, Volume and Issue: 161(13)

Published: Oct. 2, 2024

Liquid–liquid phase separation plays a prominent role in the physics of life, providing cells with various membrane-less compartments. These structures exhibit range material properties that, many cases, change over time. Inspired by this, we investigate here an aqueous two-phase system formed mixing polyethylene glycol dextran. We modulate resulting dextran droplets adding DNA that readily enters droplets. find non-monotonic dependence physical under imposed ionic conditions.

Language: Английский

Citations

0
Ferguson plot analysis of chaperone ClpB from moderate halophile DOI Creative Commons
Teruo Akuta,

Yui Tomioka,

Tomoto Ura

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 8, 2024

Abstract Ferguson plot is a simple method to determine the molecular weight of native proteins and their complexes. We here tested validity based on agarose gel electrophoresis using multimeric chaperone protein, ClpB, derived from moderate halophile that forms hexamer. showed single band with 1,500 kDa, about twice size hexamer, which may be consistent structure other chaperons form double ring comprising dimer two hexamer assembly units, i.e, dodecamer. In fact, dynamic light scattering experiment peaks, appears correspond dodecamer structures.

Language: Английский

Citations

0
Polymer Solutions in Microflows: Tracking and Control over Size Distribution DOI Open Access
Artem Bezrukov, Yu. G. Galyametdinov

Polymers, Journal Year: 2024, Volume and Issue: 17(1), P. 28 - 28

Published: Dec. 26, 2024

Microfluidics provides cutting-edge technological advancements for the in-channel manipulation and analysis of dissolved macromolecular species. The intrinsic potential microfluidic devices to control key characteristics polymer macromolecules such as their size distribution requires unleashing its full capacity. This work proposes a combined approach analyzing microscale behavior solutions modifying properties. We utilized idea modeling cross-channel diffusion in polydisperse microflows using dynamic light scattering curves source data. model was implemented into Matlab script which predicts changes at chip outputs. verified predictions experiments with series microchips by detecting optical responses injected nematic liquid crystals presence species after processing. results offer new approaches tuning dispersity solution, developing auxiliary tools techniques scattering, labs-on-chips diagnostics processing polymers.

Language: Английский

Citations

0