Diagnostic Modalities in Critical Care: Point-of-Care Approach DOI Creative Commons
Saša Rajšić, Robert Breitkopf, Mirjam Bachler

et al.

Diagnostics, Journal Year: 2021, Volume and Issue: 11(12), P. 2202 - 2202

Published: Nov. 25, 2021

The concept of intensive care units (ICU) has existed for almost 70 years, with outstanding development progress in the last decades. Multidisciplinary critically ill patients become an integral part every modern health system, ensuing improved and reduced mortality. Early recognition severe medical surgical illnesses, advanced prehospital organized immediate trauma centres led to a rise ICU patients. Due underlying disease its need complex mechanical support monitoring treatment, it is often necessary facilitate bed-side diagnostics. Immediate diagnostics are essential successful treatment life threatening conditions, early complications good quality care. Management incomprehensible without continuous sophisticated monitoring, bedside ultrasonography, diverse radiologic diagnostics, blood gas analysis, coagulation management, laboratory other point-of-care (POC) diagnostic modalities. Moreover, time acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, particular attention given POC techniques due additional concerns related risk infection transmission, patient healthcare workers safety potential adverse events relocation. This review summarizes most actual information on possible modalities critical care, special focus importance approach imaging procedures.

Language: Английский

Advanced Point‐of‐Care Testing Technologies for Human Acute Respiratory Virus Detection DOI

Zhaowei Zhang,

Peng Ma, Rajib Ahmed

et al.

Advanced Materials, Journal Year: 2021, Volume and Issue: 34(1)

Published: Oct. 8, 2021

Abstract The ever‐growing global threats to human life caused by the acute respiratory virus (RV) infections have cost billions of lives, created a significant economic burden, and shaped society for centuries. timely response emerging RVs could save lives reduce medical care burden. development RV detection technologies is essential potentially preventing pandemic epidemics. However, commonly used lack sensitivity, specificity, speed, thus often failing provide rapid turnaround times. To address this problem, new are devised performance inadequacies traditional methods. These offer improvements in convenience, flexibility, portability point‐of‐care test (POCT). Herein, recent developments POCT comprehensively reviewed eight typical viruses. This review discusses challenges opportunities various recognition strategies these according their principles, including nucleic acid amplification, optical POCT, electrochemistry, lateral flow assays, microfluidics, enzyme‐linked immunosorbent microarrays. importance limits detection, throughput, portability, specificity when testing clinical samples resource‐limited settings emphasized. Finally, evaluation commercial kits both diagnosis clinical‐oriented practices included.

Language: Английский

Citations

172
Ultrasensitive lateral-flow assays via plasmonically active antibody-conjugated fluorescent nanoparticles DOI Open Access
Rohit Gupta, Prashant Gupta, Sean Wang

et al.

Nature Biomedical Engineering, Journal Year: 2023, Volume and Issue: 7(12), P. 1556 - 1570

Published: Feb. 2, 2023

Language: Английский

Citations

103
Label-Free Spectroscopic SARS-CoV-2 Detection on Versatile Nanoimprinted Substrates DOI
Debadrita Paria, Kam Sang Kwok, Piyush Raj

et al.

Nano Letters, Journal Year: 2022, Volume and Issue: 22(9), P. 3620 - 3627

Published: March 29, 2022

Widespread testing and isolation of infected patients is a cornerstone viral outbreak management, as underscored during the ongoing COVID-19 pandemic. Here, we report large-area label-free platform that combines surface-enhanced Raman spectroscopy machine learning for rapid accurate detection SARS-CoV-2. Spectroscopic signatures acquired from virus samples on metal–insulator–metal nanostructures, fabricated using nanoimprint lithography transfer printing, can provide test results within 25 min. Not only our technique accurately distinguish between different respiratory nonrespiratory viruses, but it also detect in physiologically relevant matrices such human saliva without any additional sample preparation. Furthermore, large area nanopatterning approach allows sensors to be flexible surfaces allowing them mounted surface or used wearables. We envision versatile portable spectroscopic will offer an important tool future preparedness.

Language: Английский

Citations

81
Machine learning-assisted optical nano-sensor arrays in microorganism analysis DOI
Jianyu Yang, Shasha Lu, Bo Chen

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 159, P. 116945 - 116945

Published: Jan. 20, 2023

Language: Английский

Citations

46
Micrometer-thick and porous nanocomposite coating for electrochemical sensors with exceptional antifouling and electroconducting properties DOI Creative Commons

Jeong‐Chan Lee,

Su Yeong Kim,

Jayeon Song

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 8, 2024

Abstract Development of coating technologies for electrochemical sensors that consistently exhibit antifouling activities in diverse and complex biological environments over extended time is vital effective medical devices diagnostics. Here, we describe a micrometer-thick, porous nanocomposite with both electroconducting properties enhances the sensitivity sensors. Nozzle printing oil-in-water emulsion used to create 1 micrometer thick composed cross-linked albumin interconnected pores gold nanowires. The layer resists biofouling maintains rapid electron transfer kinetics one month when exposed directly fluids, including serum nasopharyngeal secretions. Compared thinner (nanometer thick) made drop casting or spin same thickness, sensor exhibits sensitivities are enhanced by 3.75- 17-fold three different target biomolecules tested. As result, emulsion-coated, multiplexed can carry out simultaneous detection severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid, antigen, host antibody clinical specimens high specificity. This technology holds promise addressing hurdles currently restricting application point-of-care diagnostics, implantable devices, other healthcare monitoring systems.

Language: Английский

Citations

24
Amplification-free, OR-gated CRISPR-Cascade reaction for pathogen detection in blood samples DOI Creative Commons
Jongwon Lim, A Van,

Katherine Koprowski

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(11)

Published: March 10, 2025

Rapid and accurate detection of DNA from disease-causing pathogens is essential for controlling the spread infections administering timely treatments. While traditional molecular diagnostics techniques like PCR are highly sensitive, they include nucleic acid amplification many need to be performed in centralized laboratories, limiting their utility point-of-care settings. Recent advances CRISPR-based (CRISPR-Dx) have demonstrated potential specific detection, but sensitivity often constrained by slow trans-cleavage activity Cas enzymes, necessitating preamplification target acids. In this study, we present a CRISPR-Cascade assay that overcomes these limitations integrating positive feedback loop enables amplification-free pathogenic at atto-molar levels achieves signal-to-noise ratio greater than 1.3 within just 10 min. The versatility through bloodstream infection pathogens, including Methicillin-Sensitive Staphylococcus aureus (MSSA), Methicillin-Resistant (MRSA), Escherichia coli , Hepatitis B Virus (HBV) spiked whole blood samples. Additionally, introduce multiplexing OR-function logic gate, further enhancing rapid clinical Our findings highlight ability provide sensitive paving way advanced applications beyond.

Language: Английский

Citations

2
Detection of SARS-CoV-2 Virus Amplification Using a Crumpled Graphene Field-Effect Transistor Biosensor DOI
Insu Park, Jongwon Lim, Seungyong You

et al.

ACS Sensors, Journal Year: 2021, Volume and Issue: 6(12), P. 4461 - 4470

Published: Dec. 8, 2021

The rapid and unexpected spread of SARS-CoV-2 worldwide has caused unprecedented disruption to daily life brought forward critical challenges for public health. disease was the largest cause death in United States early 2021. Likewise, COVID-19 pandemic highlighted need accurate diagnoses at scales larger than ever before. To improve availability current gold standard diagnostic testing methods, development point-of-care devices that can maintain sensitivity while reducing cost providing portability is much needed. In this work, we combine amplification capabilities reverse transcriptase loop-mediated isothermal (RT-LAMP) techniques with high-sensitivity end-point detection crumpled graphene field-effect transistors (cgFETs) develop a portable cell. This electrical method takes advantage ability adsorb single-stranded DNA due noncovalent π-π bonds but not double-stranded DNA. These have demonstrated detect presence virus range from 10 104 copies/μL 20 viral transport medium (VTM) clinical samples. As result, achieved 100% PPV, NPV, sensitivity, specificity positive negative VTM Further, cgFET differentiate between samples 35 min based on Dirac point shift. improved sensing gFET were compared those traditional flat devices.

Language: Английский

Citations

67
A rapid RNA extraction-free lateral flow assay for molecular point-of-care detection of SARS-CoV-2 augmented by chemical probes DOI
Ketan Dighe, Parikshit Moitra, Maha Alafeef

et al.

Biosensors and Bioelectronics, Journal Year: 2021, Volume and Issue: 200, P. 113900 - 113900

Published: Dec. 18, 2021

Language: Английский

Citations

60
Ferrobotic swarms enable accessible and adaptable automated viral testing DOI Open Access
Haisong Lin, Wenzhuo Yu,

Kiarash A. Sabet

et al.

Nature, Journal Year: 2022, Volume and Issue: 611(7936), P. 570 - 577

Published: Nov. 9, 2022

Language: Английский

Citations

57
Highly Stable Buffer-Based Zinc Oxide/Reduced Graphene Oxide Nanosurface Chemistry for Rapid Immunosensing of SARS-CoV-2 Antigens DOI
Fatemeh Haghayegh, Razieh Salahandish, Mohsen Hassani

et al.

ACS Applied Materials & Interfaces, Journal Year: 2022, Volume and Issue: 14(8), P. 10844 - 10855

Published: Feb. 17, 2022

The widespread and long-lasting effect of the COVID-19 pandemic has called attention to significance technological advances in rapid diagnosis SARS-CoV-2 virus. This study reports use a highly stable buffer-based zinc oxide/reduced graphene oxide (bbZnO/rGO) nanocomposite coated on carbon screen-printed electrodes for electrochemical immuno-biosensing nuelocapsid (N-) protein antigens spiked clinical samples. incorporation salt-based (ionic) matrix uniform dispersion nanomixture eliminates multistep nanomaterial synthesis surface electrode enables single-step sensor nanocoating. immuno-biosensor provides limit detection 21 fg/mL over linear range 1-10 000 pg/mL exhibits sensitivity 32.07 ohms·mL/pg·mm2 N-protein biosensor is successful discriminating positive negative samples within 15 min, demonstrating its proof concept used as antigen test.

Language: Английский

Citations

53