Carbon Dots: From Synthesis to Unraveling the Fluorescence Mechanism

Small, Journal Year: 2023, Volume and Issue: 20(4)

Published: Sept. 15, 2023

Carbon dots (CDs) being a new type of carbon-based nanomaterial have attracted intensive interest from researchers owing to their excellent biophysical properties. CDs are class fluorescent carbon nanomaterials that emerged as promising alternative traditional quantum and organic dyes in applications including bioimaging, sensing, optoelectronics. possess unique optical properties, such tunable emission, facile synthesis, low toxicity, making them attractive for many biology, medicine, environmental areas. The synthesis is achievable by variety methods, bottom-up top-down approaches, involving the use different sources surface functionalization strategies. However, understanding fluorescence mechanism remains challenge. Various mechanistic models been proposed explain origin luminescence. This review summarizes recent developments provides an overview current mechanism.

Language: Английский

---

Recent advances in point-of-care testing of COVID-19

Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 52(24), P. 8500 - 8530

Published: Jan. 1, 2023

This review explores various point-of-care optical diagnostic systems combined with microdevices developed during the recent COVID-19 pandemic for clinical diagnostics.

Language: Английский

---

Low-Cost Biosensor Technologies for Rapid Detection of COVID-19 and Future Pandemics

ACS Nano, Journal Year: 2024, Volume and Issue: 18(3), P. 1757 - 1777

Published: Jan. 8, 2024

Many systems have been designed for the detection of SARS-CoV-2, which is virus that causes COVID-19. SARS-CoV-2 readily transmitted, resulting in rapid spread disease human populations. Frequent testing at point care (POC) a key aspect controlling outbreaks caused by and other emerging pathogens, as early identification infected individuals can then be followed appropriate measures isolation or treatment, maximizing chances recovery preventing infectious spread. Diagnostic tools used high-frequency should inexpensive, provide diagnostic response without sophisticated equipment, amenable to manufacturing on large scale. The application these devices enable large-scale data collection, help control viral transmission, prevent propagation. Here we review functional nanomaterial-based optical electrochemical biosensors accessible POC These incorporate nanomaterials coupled with paper-based analytical inexpensive substrates, traditional lateral flow technology (antigen antibody immunoassays), innovative biosensing methods. We critically discuss advantages disadvantages nanobiosensor-based approaches compared widely technologies such PCR, ELISA, LAMP. Moreover, delineate main technological, (bio)chemical, translational, regulatory challenges associated developing reliable biosensors, prevented their translation into clinic. Finally, highlight how nanobiosensors, given unique over existing tests, may future pandemics.

Language: Английский

---

Robust Rules for Optimal Colorimetric Sensing Based on Gold Nanoparticle Aggregation

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(4), P. 1827 - 1834

Published: April 13, 2023

Spurred by outstanding optical properties, chemical stability, and facile bioconjugation, plasmonic metals have become the first-choice materials for signal transducers in biosensing. While design rules surface-based sensors are well-established commercialized, there is limited knowledge of based on nanoparticle aggregation. The reason lack control over interparticle distances, number nanoparticles per cluster, or multiple mutual orientations during aggregation events, blurring threshold between positive negative readout. Here we identify geometrical parameters (size, shape, distance) that allow maximizing color difference upon clustering. Finding optimal structural will provide a fast reliable means readout, including unaided eye inspection computer vision.

Language: Английский

---

How can lessons from the COVID-19 pandemic enhance antimicrobial resistance surveillance and stewardship?

The Lancet Infectious Diseases, Journal Year: 2023, Volume and Issue: 23(8), P. e301 - e309

Published: June 5, 2023

Language: Английский

---

Rapid assays of SARS-CoV-2 virus and noble biosensors by nanomaterials

Nano Convergence, Journal Year: 2024, Volume and Issue: 11(1)

Published: Jan. 8, 2024

Abstract The COVID-19 outbreak caused by SARS-CoV-2 in late 2019 has spread rapidly across the world to form a global epidemic of respiratory infectious diseases. Increased investigations on diagnostic tools are currently implemented assist rapid identification virus because mass and diagnosis might be best way prevent virus. This critical review discusses detection principles, fabrication techniques, applications with three categories: nuclear acid augmentation test, immunoassay test biosensors. Special efforts were put enhancement nanomaterials biosensors for rapid, sensitive, low-cost diagnostics Future developments suggested regarding potential candidates hospitals, clinics laboratories control prevention large-scale epidemic. Graphical

Language: Английский

---

Exploring precision treatments in immune‐mediated inflammatory diseases: Harnessing the infinite potential of nucleic acid delivery

Exploration, Journal Year: 2024, Volume and Issue: unknown

Published: May 24, 2024

Immune-mediated inflammatory diseases (IMIDs) impose an immeasurable burden on individuals and society. While the conventional use of immunosuppressants disease-modifying drugs has provided partial relief control, their inevitable side effects limited efficacy cast a shadow over finding cure. Promising nucleic acid have shown potential to exert precise at molecular level, with different classes acids having regulatory functions through varying mechanisms. For better delivery acids, safe effective viral vectors non-viral systems (including liposomes, polymers, etc.) been intensively explored. Herein, after describing range categories vectors, we focus application therapeutic in various IMIDs, including rheumatoid arthritis, bowel disease, psoriasis, multiple sclerosis, asthma, ankylosing spondylitis, systemic lupus erythematosus, uveitis. Molecules implicated inflammation immune dysregulation are abnormally expressed series meticulous modulation therapy results degrees remission improvement these diseases. By synthesizing findings centered specific targets, this review delivers systematic elucidation perspective towards advancing utilization therapeutics for managing IMIDs.

Language: Английский

---

Building the Future of Clinical Diagnostics: An Analysis of Potential Benefits and Current Barriers in CRISPR/Cas Diagnostics

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Advancements in molecular diagnostics, such as polymerase chain reaction and next-generation sequencing, have revolutionized disease management prognosis. Despite these advancements the field faces challenges due to high operational costs need for sophisticated equipment highly trained personnel besides having several technical limitations. The emergent of CRISPR/Cas sensing technology is showing promise a new paradigm clinical although widespread adoption remains limited. This perspective paper discusses specific cases where can surmount existing diagnostic methods by stressing significant role that play revolutionizing diagnostics. It underscores urgency importance addressing technological regulatory hurdles must be overcome harness this effectively laboratories.

Language: Английский

---

Detection of COVID-19: A Smartphone-Based Machine-Learning-Assisted ECL Immunoassay Approach with the Ability of RT-PCR CT Value Prediction

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(47), P. 16361 - 16368

Published: Nov. 16, 2022

The unstoppable spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has severely threatened public health over the past years. current ubiquitously accepted method for its diagnosis provides sensitive detection virus; however, it is relatively time-consuming and costly, not to mention need highly skilled personnel. There a clear develop novel computer-based diagnostic tools provide rapid, cost-efficient, time-saving in places where massive traditional testing practical. Here, we an electrochemiluminescence (ECL)-based system whose results are quantified as reverse transcriptase polymerase chain reaction (RT-PCR) cyclic threshold (CT) values. A concentration-dependent signal generated upon introduction virus electrode recorded with smartphone camera. ECL images used train machine learning algorithms, model using artificial neural networks (ANNs) 45 samples was developed. demonstrated more than 90% accuracy 50 unknown real samples, detecting up CT value 32 limit (LOD) 10–12 g mL–1 samples.

Language: Английский

---

An ultrasensitive ELISA to assay femtomolar level SARS-CoV-2 antigen based on specific peptide and tyramine signal amplification

Sensors and Actuators B Chemical, Journal Year: 2023, Volume and Issue: 387, P. 133746 - 133746

Published: March 29, 2023

Language: Английский

---