Ultrasound-Augmented Multienzyme-like Nanozyme Hydrogel Spray for Promoting Diabetic Wound Healing

ACS Nano, Journal Year: 2023, Volume and Issue: 17(16), P. 15962 - 15977

Published: Aug. 3, 2023

Treatment of diabetic foot ulcers (DFU) needs to reduce inflammation, relieve hypoxia, lower blood glucose, promote angiogenesis, and eliminate pathogenic bacteria, but the therapeutic efficacy is greatly limited by diversity synergy drug functions as well DFU microenvironment itself. Herein, an ultrasound-augmented multienzyme-like nanozyme hydrogel spray was developed using hyaluronic acid encapsulated l-arginine ultrasmall gold nanoparticles Cu1.6O coloaded phosphorus doped graphitic carbon nitride nanosheets (ACPCAH). This possesses five types enzyme-like activities, including superoxide dismutase (SOD)-, catalase (CAT)-, glucose oxidase (GOx)-, peroxidase (POD)-, nitric oxide synthase (NOS)-like activities. The kinetics reaction mechanism sonodynamic/sonothermal synergistic enhancement SOD-CAT-GOx-POD/NOS cascade ACPCAH are fully investigated. Both in vitro vivo tests demonstrate that this can be activated thus accelerating wound healing effectively. study highlights a competitive approach based on nanozymes for development all-in-one therapies.

Language: Английский

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Mimicking Antioxidases and Hyaluronan Synthase: A Zwitterionic Nanozyme for Photothermal Therapy of Osteoarthritis

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(44)

Published: July 17, 2023

Abstract Restoring joint homeostasis is crucial for relieving osteoarthritis (OA). Current strategies are limited to unilateral efforts in lubrication, inhibition of inflammation, free radicals scavenging, and cartilage regeneration. Herein, by modifying molybdenum disulfide (MoS 2 ) with Mg 2+ ‐doped polydopamine coating polysulfobetaines, a dual‐bionic photothermal nanozyme (MPMP) constructed mimic antioxidases/hyaluronan synthase OA therapy. Photothermally enhanced lubrication lowers the coefficient friction (0.028) early stage treatment. The antioxidases‐mimicking properties MPMP contribute eliminating reactive oxygen nitrogen species (ROS/RNS) (over 90% scavenging ratio H O /·OH/O · – /DPPH/ABTS + supplying . With NIR irradiation, triggers thermogenesis (upregulating HSP70 expression) release, which promotes chondrogenesis inflammatory conditions deactivating NF‐κB/IL‐17 signaling pathways enhancing MAPK pathway. Benefiting from , MPMP‐NIR shows HAS‐mimicking activity increase intracellular (twofold) extracellular (3.12‐fold) HA production. Therefore, demonstrates superior spatiotemporally therapeutic effect on mice model, terms osteophytes (83.41% reduction), OARSI scores (88.57% ACAN expression (2.70‐fold increment). Hence, insights into nanozymes can be promising strategy therapy or other inflammation‐related diseases.

Language: Английский

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Cerium–Luteolin Nanocomplexes in Managing Inflammation-Related Diseases by Antioxidant and Immunoregulation

ACS Nano, Journal Year: 2024, Volume and Issue: 18(8), P. 6229 - 6242

Published: Feb. 12, 2024

Oxidative stress, characterized by an imbalance between reactive oxygen species (ROS) production and the antioxidant defense system, plays a pivotal role in inflammation-related diseases. Excessive ROS levels can induce cellular damage impair normal physiological functions, triggering release of inflammatory mediators exacerbating response, ultimately leading to irreversible tissue damage. In this study, we synthesized cerium ion–luteolin nanocomplexes (CeLutNCs) coordinating Ce ions with natural product luteolin, aiming develop therapeutic agent excellent immunoregulation properties for ROS-related inflammation treatment. vitro experiments demonstrated that prepared CeLutNCs effectively scavenged excess ROS, prevented cell apoptosis, down-regulated important cytokines, regulated response macrophages, suppressed activation nuclear factor-κ-gene binding (NF-κB) pathway. acute kidney injury (AKI) animal model, exhibited significant efficacy improving function, repairing damaged renal tissue, reducing oxidative apoptosis. Moreover, potential lung (ALI) model was confirmed through assessment responses histopathological studies. This study emphasizes effectiveness these metal–natural coordination as promising approach preventing AKI other diseases associated stress.

Language: Английский

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Precise modulation and use of reactive oxygen species for immunotherapy

Science Advances, Journal Year: 2024, Volume and Issue: 10(20)

Published: May 15, 2024

Reactive oxygen species (ROS) play an important role in regulating the immune system by affecting pathogens, cancer cells, and cells. Recent advances biomaterials have leveraged this mechanism to precisely modulate ROS levels target tissues for improving effectiveness of immunotherapies infectious diseases, cancer, autoimmune diseases. Moreover, ROS-responsive can trigger release immunotherapeutics provide tunable kinetics, which further boost their efficacy. This review will discuss latest biomaterial-based approaches both precise modulation using as a stimulus control kinetics immunotherapeutics. Finally, we existing challenges potential solutions clinical translation ROS-modulating immunotherapy, outlook future research.

Language: Английский

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Inflammation‐Responsive Hydrogel Accelerates Diabetic Wound Healing through Immunoregulation and Enhanced Angiogenesis

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: April 25, 2024

Angiogenesis is a prominent component during the highly regulated process of wound healing. The application exogenous vascular endothelial growth factor (VEGF) has shown considerable potential in facilitating angiogenesis. However, its effectiveness often curtailed due to chronic inflammation and severe oxidative stress diabetic wounds. Herein, an inflammation-responsive hydrogel incorporating Prussian blue nanoparticles (PBNPs) designed augment angiogenic efficacy VEGF. Specifically, rapid release PBNPs from under inflammatory conditions effectively alleviates wound, therefore reprogramming immune microenvironment preserve bioactivity VEGF for enhanced In vitro vivo studies reveal that co-loaded biocompatible possesses effective anti-inflammatory properties, thereby angiogenesis accelerate healing type 2 mouse model.

Language: Английский

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Inflammation-Responsive Nanoagents for Activatable Photoacoustic Molecular Imaging and Tandem Therapies in Rheumatoid Arthritis

ACS Nano, Journal Year: 2024, Volume and Issue: 18(3), P. 2231 - 2249

Published: Jan. 8, 2024

Rheumatoid arthritis (RA) severely lowers the life quality by progressively destructing joint functions and eventually causing permanent disability, representing a pressing public health concern. The pathogenesis of RA includes excessive production proinflammatory cytokines harmful oxygen-derived free radicals, such as nitric oxide (NO), which constitute vital targets for precise diagnosis effective treatment RA. In this study, we introduce an advanced nanoagent that integrates microenvironment-activatable photoacoustic (PA) imaging with multitarget synergistic A highly sensitive organic probe NO-tunable energy transformation molecular geometry is developed, enables strong near-infrared absorption turn-on PA signal, active intramolecular motion could further boost conversion. coassembled inflammation-responsive prodrug to construct theranostic nanoagent, on macrophage-derived cell membrane natural tropism inflammatory sites camouflaged improve targeting ability inflamed joints. not only sensitively detect differentiate severity but also efficiently alleviate symptoms function. combination activatable probe-mediated NO scavenging on-demand activation anti-inflammatory significantly inhibits factors promotes macrophage repolarization from M1 M2 phenotype. This meticulously designed ingeniously RA-specific therapy, rendering tremendous promise intervention RA-related diseases.

Language: Английский

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“Smart” stimuli-responsive biomaterials revolutionizing the theranostic landscape of inflammatory arthritis

Materials Today Chemistry, Journal Year: 2024, Volume and Issue: 39, P. 102178 - 102178

Published: July 1, 2024

Inflammatory arthritis is a growing worldwide concern, afflicting millions of individuals and leading to depreciating quality life, significant morbidity, subsequent healthcare burden. Conventional therapies for inflammatory often hold limitations, including systemic cross-reactivities, side effects, inadequate management inflammation. Therefore, "Smart" stimuli-responsive biomaterials are materialized as promising theranostic approaches management. These designed specific external internal stimulation, enabling the sustained release bioactive therapeutic agents during local or administration. Stimuli-responsive allow precise spatiotemporal control over drug release, minimizing exposure, reducing off-target real-time monitoring pathological conditions, driving potential breakthrough in managing arthritis. Owing this prominence, review article expounds on emerging prospects targeting molecular signatures associated with intricately elicit minimal no contradictory effects. In addition, application these disease progression multi-stimuli-responsive comprehended show progress diagnosis treatment. This "single-platted" comprehensive acquaintance clinical-based outcomes navigates microenvironment, proposing an optimistic avenue personalized effective minimized

Language: Английский

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Inhibition of macrophage polarization and pyroptosis in collagen-induced arthritis through MSC-exo and ginsenoside Rh2

Arthritis Research & Therapy, Journal Year: 2025, Volume and Issue: 27(1)

Published: Jan. 9, 2025

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint inflammation, tissue damage, and fibrosis, significantly affecting the quality of life. While there are currently some effective treatments available, they often come with side effects. There an urgent need to find new that can further improve therapeutic outcomes reduce Our study investigates role Mesenchymal Stem Cell exosomes (MSC-exo) combined Ginsenoside Rh2 (Rh2) in treatment RA. We specifically focus on how this strategy influences macrophage polarization pyroptosis. This research utilized collagen-induced rat model. The findings reveal combination MSC-exo inhibit M1 macrophages, increase proportion M2-like suppress M1-like pyroptosis via NLRP3/Caspase11/GSDMD-N pathway. In model, showed synergistic contributes deeper understanding RA's pathogenesis presents potential targeted interventions. application offers promising insights for future innovative strategies RA treatment, paving way more management disease.

Language: Английский

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High-Throughput Screening Strategy and Metal–Organic Framework-Based Multifunctional Controlled-Release Nanomaterial for Osteoarthritis Therapy

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 19, 2025

Osteoarthritis (OA) is a prevalent degenerative disease that lacks effective therapy. Oxidative stress one of the major factors contributing to OA; however, treatments targeting oxidative are still lacking. In current study, we established an stress-induced cell death model in chondrocytes vitro and screened drugs may suppress death. Ethyl gallate (EG) was identified as most potent drug against out more than 600 natural product library. Application without appropriate delivery system for OA therapy have drawbacks such low bioavailability, short action time, poor efficacy. Herein, poly-His6-zinc assembly (PZA), pH-responsive metal–organic framework (MOF) loaded with EG (EG@PZA) designed It demonstrated EG@PZA lysosome escape property, which dramatically increases utilization EG. Furthermore, showed enhanced release capability acidic microenvironment. vivo studies effectively suppresses extracellular matrix degradation, ferroptosis, senescence also ameliorates destabilization medial meniscus (DMM) mouse vivo. Together, study become potential controlled-release nanomaterial

Language: Английский

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Retinal Microenvironment‐Protected Rhein‐GFFYE Nanofibers Attenuate Retinal Ischemia‐Reperfusion Injury via Inhibiting Oxidative Stress and Regulating Microglial/Macrophage M1/M2 Polarization

Advanced Science, Journal Year: 2023, Volume and Issue: 10(30)

Published: Aug. 31, 2023

Retinal ischemia is involved in the occurrence and development of various eye diseases, including glaucoma, diabetic retinopathy, central retinal artery occlusion. To best our knowledge, few studies have reported self-assembling peptide natural products for suppression ocular inflammation oxidative stress. Herein, a GFFYE designed synthesized, which can transform non-hydrophilicity rhein into an amphiphilic sustained-release therapeutic agent, rhein-based nanofibers (abbreviated as Rh-GFFYE) are constructed treatment ischemia-reperfusion (RIR) injury. Rh-GFFYE significantly ameliorates stress vitro oxygen-glucose deprivation (OGD) model rat RIR also enhances electrophysiological recovery exhibits good biocompatibility. Importantly, promotes transition M1-type macrophages to M2 type, ultimately altering pro-inflammatory microenvironment. Further investigation mechanism indicates that activates PI3K/AKT/mTOR signaling pathway reduce inhibits NF-κB STAT3 pathways affect macrophage polarization. In conclusion, rhein-loaded nanoplatform alleviates injury by modulating The findings expected promote clinical application hydrophobic injury-associated diseases.

Language: Английский

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