Precise visualization and ROS-dependent photodynamic therapy of colorectal cancer with a novel mitochondrial viscosity photosensitive fluorescent probe DOI Creative Commons
Runsha Xiao, Fan Zheng,

Kuo Kang

et al.

Biomaterials Research, Journal Year: 2023, Volume and Issue: 27(1)

Published: Feb. 9, 2023

Abstract Background Colorectal cancer (CRC) is a prominent global with high mortality rates among human beings. Efficient diagnosis and treatment have always been challenge for CRC management. Fluorescence guided therapy, which combines therapy into one platform, has brought new chance achieving precise theranostics. Among this, photosensitizers, applied in photodynamic (PDT), given the integration of real-time imaging capacity efficacious feasibility, show great potential to serve as remarkable tools. Although much effort put constructing photosensitizers locating destroying cells, it still need develop novel attain specific detection fulfil effective therapy. Methods Probe HTI was rational synthesized CRC. Spectrometric determination carried out first, followed by 1 O 2 generation ability test. Then, displayed distinguishing cells from normal Further, PDT effect photosensitizer studied vitro. Additionally, used BALB/c nude mice model validate its viscosity labelling tumor suppression characteristics. Results We successfully fabricated mitochondrial targeting probe, HTI, together sensitivity, ultralow background interference, excellent capacity. favorably detection, displaying 11-fold fluorescent intensity enhancement solvents 1.57 cp 2043 cp. demonstrated that could distinguish upon difference viscosity. Moreover, qualified producing efficiency supported sparkling signals DCFH after incubation under light irradiation. More importantly, performance CDX determined, enriching multifunctional validation vivo. Conclusions In this study, sensitive response possess Both vitro cell vivo trials proved effectively served robust scaffold labeling clearance. This breakthrough discovery held immense advancing early management through PDT. By leveraging HTI's properties, medical professionals benefit improved diagnostic accuracy targeted management, ultimately leading enhanced patient outcomes.

Language: Английский

Activatable Probes for Ratiometric Imaging of Endogenous Biomarkers In Vivo DOI
Qinrui Fu, Xiao Yang, Mengzhen Wang

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(5), P. 3916 - 3968

Published: Jan. 23, 2024

Dynamic variations in the concentration and abnormal distribution of endogenous biomarkers are strongly associated with multiple physiological pathological states. Therefore, it is crucial to design imaging systems capable real-time detection dynamic changes for accurate diagnosis effective treatment diseases. Recently, ratiometric has emerged as a widely used technique sensing due its advantage circumventing limitations inherent conventional intensity-dependent signal readout methods while also providing built-in self-calibration correction. Here, recent progress probes their applications outlined. Ratiometric classified according mechanisms, photoacoustic imaging, optical including photoluminescence self-luminescence magnetic resonance dual-modal discussed. The such pH, reactive oxygen species (ROS), nitrogen (RNS), glutathione (GSH), gas molecules, enzymes, metal ions, hypoxia discussed detail. Additionally, this Review presents an overview challenges faced field along future research directions.

Language: Английский

Citations

31
CRISPR/Cas12a-derived ratiometric fluorescence sensor for high-sensitive Pb2+ detection based on CDs@ZIF-8 and DNAzyme DOI

Chuanyu Yang,

Caiyi Du,

Feiyu Yuan

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 251, P. 116089 - 116089

Published: Feb. 3, 2024

Language: Английский

Citations

22
Rational Design of NIR‐II Ratiometric Fluorescence Probes for Accurate Bioimaging and Biosensing In Vivo DOI
Tuanwei Li, Yejun Zhang, Feng Wu

et al.

Small Methods, Journal Year: 2024, Volume and Issue: unknown

Published: March 12, 2024

Abstract Intravital fluorescence imaging in the second near‐infrared window (NIR‐II, 900–1700 nm) has emerged as a promising method for non‐invasive diagnostics complex biological systems due to its advantages of less background interference, high tissue penetration depth, contrast, and sensitivity. However, traditional NIR‐II imaging, which is characterized by “always on” or “turn mode, lacks ability quantitative detection, leading low reproducibility reliability during bio‐detection. In ratiometric can realize reliable analysis detection vivo providing reference signals correction, generating new opportunities prospects bioimaging biosensing. this review, current design strategies sensing mechanisms probes biosensing applications are systematically summarized. Further, challenges, future perspectives designing also discussed. It hoped that review provide effective guidance promote adoption vivo.

Language: Английский

Citations

20
Molecular imaging of tumour‐associated pathological biomarkers with smart nanoprobe: From “Seeing” to “Measuring” DOI Creative Commons
Peisen Zhang, Wenyue Li, Chuang Liu

et al.

Exploration, Journal Year: 2023, Volume and Issue: 3(6)

Published: Oct. 28, 2023

Although the extraordinary progress has been made in molecular biology, prevention of cancer remains arduous. Most solid tumours exhibit both spatial and temporal heterogeneity, which is difficult to be mimicked vitro. Additionally, complex biochemical immune features tumour microenvironment significantly affect development. Molecular imaging aims at exploitation tumour-associated molecules as specific targets customized probe, thereby generating image contrast markers, offering opportunities non-invasively evaluate pathological characteristics vivo. Particularly, there are no "standard markers" control clinical diagnosis individuals, so characteristics-responsive nanoprobe-based quantitative imaging, able visualize determine accurate content values heterogeneous distribution tumours, can provide criteria for diagnosis. In this context, a variety "smart" nanoprobes have designed, order feasible approaches quantitatively This review summarizes recent achievements designs these nanoprobes, highlights state-of-the-art technologies molecules.

Language: Английский

Citations

23
Nanotechnology in inflammation: cutting-edge advances in diagnostics, therapeutics and theranostics DOI Creative Commons
Yuting Liu, Ziqi Lin, Yuting Wang

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(6), P. 2490 - 2525

Published: Jan. 1, 2024

Inflammatory dysregulation is intimately associated with the occurrence and progression of many life-threatening diseases.Accurate detection timely therapeutic intervention on inflammatory are crucial for effective therapy inflammation-associated diseases.However, clinical outcomes inflammation-involved disorders still unsatisfactory.Therefore, there an urgent need to develop innovative anti-inflammatory strategies by integrating emerging technological innovations traditional therapeutics.Biomedical nanotechnology one promising fields that can potentially transform diagnosis treatment inflammation.In this review, we outline recent advances in biomedical inflammation, special attention paid nanosensors nanoprobes precise inflammation-related diseases, nanotherapeutics, as well nanotheranostics combined applications.Moreover, prospects challenges translation nanomedicines highlighted.

Language: Английский

Citations

14
Ratiometric Optical and Photoacoustic Imaging In Vivo in the Second Near-Infrared Window DOI

Kang Zhu,

Xuan Zhang, Ying Wu

et al.

Accounts of Chemical Research, Journal Year: 2023, Volume and Issue: 56(22), P. 3223 - 3234

Published: Nov. 7, 2023

ConspectusOptical imaging and photoacoustic (PA) have become essential tools to investigate physiological or pathological processes at the molecular level in vivo. The detection of variations vivo is particularly important owing rapid progression diseases. However, most studies mainly focused on plain qualitative detection, which characterized by absence a reference signal one-channel responsive imaging. To overcome limitation quantitatively detect molecules situ, this Account reviews recent contributions our group quantitative field form ratiometric optical PA second near-infrared window (NIR-II, 950–1700 nm).In Account, we present advances that has made probe design biomedical applications constructing probes based First, highlight strategies were organic probes, radio-activated hybrid organic–inorganic assembled probes. Subsequently, NIR-II nanoprobes with activated bioluminescence resonance energy transfer (BRET), Förster (FRET), nonradiative (NRET) effects provide reliable tool achieve endogenous signaling thereby apply it monitoring evaluation efficacy photodynamic therapy, radiotherapy, immunotherapy guide treatment process. In addition, systematically introduce functional principles core–shell nanoprobes, core–satellite universal where established sensing can be obtained from random assortment plasmonic components semiconducting using phase separation strategy. On these insights, discuss rational detailed include accurate disease-related inflammation tumors real time. champion implementations different diagnostic modules been linked through compound modification activation characteristics (e.g., pH, redox, enzyme, hypoxia). Finally, challenges perspectives for multitarget future clinical translation. We believe upcoming generations would promising potential precise diagnosis may stimulate innovative exploration their applications.

Language: Английский

Citations

18
Magnetic-susceptibility-dependent ratiometric probes for enhancing quantitative MRI DOI
Cheng Zhang,

Bin Nan,

Juntao Xu

et al.

Nature Biomedical Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 29, 2024

Language: Английский

Citations

7
Calcium sulfide based nanoreservoirs elicit dual pyroptosis pathways for enhanced anti-tumor immunity DOI
Qi Yu,

Q.Y. Li,

Le Tu

et al.

Chemical Engineering Journal, Journal Year: 2023, Volume and Issue: 477, P. 147085 - 147085

Published: Nov. 3, 2023

Language: Английский

Citations

11
Visualization of Drug Release in a Chemo-Immunotherapy Nanoplatform via Ratiometric 19F Magnetic Resonance Imaging DOI Creative Commons
Fanqi Liu, Xindi Li,

Yumin Li

et al.

Chemical Science, Journal Year: 2024, Volume and Issue: 15(42), P. 17397 - 17406

Published: Jan. 1, 2024

Visualization of drug release

Language: Английский

Citations

4
A Sophisticated Ratiometric Nuclear Medicine Imaging Strategy for Biological Microenvironment Abnormal Factor Detection DOI Open Access
Jianxian Ge, H. Jerry Qi, Lishu Zhao

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

Biological microenvironment detection is crucial for deciphering the mechanisms underlying malignant progression and predicting treatment efficacy of diseases. Nevertheless, only very limited progress has been made toward non-invasive quantitative abnormal factors, let alone with clinically compatible imaging modalities. Herein, a smart nuclear medicine probe proposed, innovatively designed visualization glutathione (GSH) in vivo. This contains disulfide bond that links two molecular segments labeled 125I 177Lu, respectively. Upon systemic delivery, preferentially accumulates liver, where GSH cleaves it into fragments completely different metabolic fates: one retained at response site other rapidly excreted. unique feature provides an opportunity to use 177Lu/125I signal ratio non-invasively characterize concentration vivo, enabling highly sensitive quantification strongly associated many hepatic Moreover, strategy also reliable method levels tumors. It thus believed current study groundbreaking quantitatively revealing disease-related not GSH,

Language: Английский

Citations

0